LOCUS       sequence-88649-e        1193 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 505 / 569, Emission: 516
            / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1193)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 1193)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEos4b-H2B-6
FEATURES             Location/Qualifiers
     source          1..1193
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             69..446
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      459..468
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             465..1148
                     /codon_start=1
                     /product="fixation-resistant truly monomeric green-to-red 
                     photoswitchable fluorescent protein (Paez-Segala et al., 
                     2015)"
                     /label=mEos4b
                     /note="derived from mEos2"
                     /translation="MVSAIKPDMRIKLRMEGNVNGHHFVIDGDGTGKPYEGKQTMDLEV
                     KEGGPLPFAFDILTTAFHYGNRVFVKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNA
                     RNDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIEMALL
                     LEGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHAIEILSHDKDYNKVKLYEHAVAHSGLP
                     DNARR"
ORIGIN
        1 ccgctagcgc taccggactc agatctcgag ctcaagcttc gaattctgca gtcgacggta
       61 ccgccaccat gccagagcca gcgaagtctg ctcccgcccc gaaaaagggc tccaagaagg
      121 cggtgactaa ggcgcagaag aaaggcggca agaagcgcaa gcgcagccgc aaggagagct
      181 attccatcta tgtgtacaag gttctgaagc aggtccaccc tgacaccggc atttcgtcca
      241 aggccatggg catcatgaat tcgtttgtga acgacatttt cgagcgcatc gcaggtgagg
      301 cttcccgcct ggcgcattac aacaagcgct cgaccatcac ctccagggag atccagacgg
      361 ccgtgcgcct gctgctgcct ggggagttgg ccaagcacgc cgtgtccgag ggtactaagg
      421 ccatcaccaa gtacaccagc gctaaggatc caccggtcgc caccatggtg agtgcgatta
      481 agccagacat gaggatcaaa ctccgtatgg aaggcaacgt aaacgggcac cactttgtga
      541 tcgacggaga tggtacaggc aagccttatg agggaaaaca gaccatggat cttgaagtca
      601 aagagggcgg acctctgcct tttgcctttg atatcctgac cactgcattc cattacggca
      661 acagggtatt cgtgaaatat ccagacaaca tacaagacta ttttaagcag tcgtttccta
      721 aggggtattc gtgggaacga agcttgactt tcgaagacgg gggcatttgc aatgccagaa
      781 acgacataac aatggaaggg gacactttct ataataaagt tcgattttat ggtaccaact
      841 ttcccgccaa tggtccagtt atgcagaaga agacgctgaa atgggagccc tccactgaga
      901 aaatgtatgt gcgtgatgga gtgctgacgg gtgatattga gatggctttg ttgcttgaag
      961 gaaatgccca ttaccgatgt gacttcagaa ctacttacaa agctaaggag aagggtgtca
     1021 agttaccagg cgcccacttt gtggaccacg ccattgagat tttaagccat gacaaagatt
     1081 acaacaaggt taagctgtat gagcatgctg ttgctcattc tggattgcct gacaatgcca
     1141 gacgataagc ggccgcgact ctagatcata atcagccata ccacatttta gaa
//
