LOCUS       sequence-88650-e        1199 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 505 / 569, Emission: 516
            / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1199)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 1199)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEos4b-H2B-C-10
FEATURES             Location/Qualifiers
     source          1..1199
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      43..52
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             49..729
                     /codon_start=1
                     /product="fixation-resistant truly monomeric green-to-red 
                     photoswitchable fluorescent protein (Paez-Segala et al., 
                     2015)"
                     /label=mEos4b
                     /note="derived from mEos2"
                     /translation="MVSAIKPDMRIKLRMEGNVNGHHFVIDGDGTGKPYEGKQTMDLEV
                     KEGGPLPFAFDILTTAFHYGNRVFVKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNA
                     RNDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIEMALL
                     LEGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHAIEILSHDKDYNKVKLYEHAVAHSGLP
                     DNARR"
     CDS             760..1137
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="PEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKVL
                     KQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLPG
                     ELAKHAVSEGTKAITKYTSAK"
ORIGIN
        1 cgcccttgtc tagtgacgtc agatccgcta gcgctaccgg tcgccaccat ggtgagtgcg
       61 attaagccag acatgaggat caaactccgt atggaaggca acgtaaacgg gcaccacttt
      121 gtgatcgacg gagatggtac aggcaagcct tatgagggaa aacagaccat ggatcttgaa
      181 gtcaaagagg gcggacctct gccttttgcc tttgatatcc tgaccactgc attccattac
      241 ggcaacaggg tattcgtgaa atatccagac aacatacaag actattttaa gcagtcgttt
      301 cctaaggggt attcgtggga acgaagcttg actttcgaag acgggggcat ttgcaatgcc
      361 agaaacgaca taacaatgga aggggacact ttctataata aagttcgatt ttatggtacc
      421 aactttcccg ccaatggtcc agttatgcag aagaagacgc tgaaatggga gccctccact
      481 gagaaaatgt atgtgcgtga tggagtgctg acgggtgata ttgagatggc tttgttgctt
      541 gaaggaaatg cccattaccg atgtgacttc agaactactt acaaagctaa ggagaagggt
      601 gtcaagttac caggcgccca ctttgtggac cacgccattg agattttaag ccatgacaaa
      661 gattacaaca aggttaagct gtatgagcat gctgttgctc attctggatt gcctgacaat
      721 gccagacgat ccggactcag atctggaggc tccggaggcc cagagccagc gaagtctgct
      781 cccgccccga aaaagggctc caagaaggcg gtgactaagg cgcagaagaa aggcggcaag
      841 aagcgcaagc gcagccgcaa ggagagctat tccatctatg tgtacaaggt tctgaagcag
      901 gtccaccctg acaccggcat ttcgtccaag gccatgggca tcatgaattc gtttgtgaac
      961 gacattttcg agcgcatcgc aggtgaggct tcccgcctgg cgcattacaa caagcgctcg
     1021 accatcacct ccagggagat ccagacggcc gtgcgcctgc tgctgcctgg ggagttggcc
     1081 aagcacgccg tgtccgaggg tactaaggcc atcaccaagt acaccagcgc taagtaagga
     1141 tccaccggat ctagataact gatcataatc agccatacca cattgtagaa ggaaatgcc
//
