LOCUS       sequence-88651-e        2087 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Note- This plasmid contains Keratin 18. Localization: Intermediate 
            Filaments, Excitation: 505 / 569, Emission: 516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2087)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEos4b-Keratin-17
FEATURES             Location/Qualifiers
     source          1..2087
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1359..1368
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1365..2048
                     /codon_start=1
                     /product="fixation-resistant truly monomeric green-to-red 
                     photoswitchable fluorescent protein (Paez-Segala et al., 
                     2015)"
                     /label=mEos4b
                     /note="derived from mEos2"
                     /translation="MVSAIKPDMRIKLRMEGNVNGHHFVIDGDGTGKPYEGKQTMDLEV
                     KEGGPLPFAFDILTTAFHYGNRVFVKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNA
                     RNDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIEMALL
                     LEGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHAIEILSHDKDYNKVKLYEHAVAHSGLP
                     DNARR"
ORIGIN
        1 gtgacgtcag atccgctagc accatgagct tcaccactcg ctccaccttc tccaccaact
       61 accggtccct gggctctgtc caggcgccca gctacggcgc ccggccggtc agcagcgcgg
      121 ccagcgtcta tgcaggcgct gggggctctg gttcccggat ctccgtgtcc cgctccacca
      181 gcttcagggg cggcatgggg tccgggggcc tggccaccgg gatagccggg ggtctggcag
      241 gaatgggagg catccagaac gagaaggaga ccatgcaaag cctgaacgac cgcctggcct
      301 cttacctgga cagagtgagg agcctggaga ccgagaaccg gaggctggag agcaaaatcc
      361 gggagcactt ggagaagaag ggaccccagg tcagagactg gagccattac ttcaagatca
      421 tcgaggacct gagggctcag atcttcgcaa atactgtgga caatgcccgc atcgttctgc
      481 agattgacaa tgcccgtctt gctgctgatg actttagagt caagtatgag acagagctgg
      541 ccatgcgcca gtctgtggag aacgacatcc atgggctccg caaggtcatt gatgacacca
      601 atatcacacg actgcagctg gagacagaga tcgaggctct caaggaggag ctgctcttca
      661 tgaagaagaa ccacgaagag gaagtaaaag gcctacaagc ccagattgcc agctctgggt
      721 tgaccgtgga ggtagatgcc cccaaatctc aggacctcgc caagatcatg gcagacatcc
      781 gggcccaata tgacgagctg gctcggaaga accgagagga gctagacaag tactggtctc
      841 agcagattga ggagagcacc acagtgctca ccacacagtc tgctgaggtt ggagctgctg
      901 agacgacgct cacagagctg agacgtacag tccagtcctt ggagatcgac ctggactcca
      961 tgagaaatct gaaggccagc ttggagaaca gcctgaggga ggtggaggcc cgctacgccc
     1021 tacagatgga gcagctcaac gggatcctgc tgcaccttga gtcagagctg gcacagaccc
     1081 gggcagaggg acagcgccag gcccaggagt atgaggccct gctgaacatc aaggtcaagc
     1141 tggaggctga gatcgccacc taccgccgcc tgctggaaga tggcgaggac tttaatcttg
     1201 gtgatgcctt ggacagcagc aactccatgc aaaccatcca aaagaccacc acccgccgga
     1261 tagtggatgg caaagtggtg tctgagacca atgacaccaa agttctgagg catcgaattc
     1321 tgcagtcgac ggtaccgcgg gcccgggatc caccggtcgc caccatggtg agtgcgatta
     1381 agccagacat gaggatcaaa ctccgtatgg aaggcaacgt aaacgggcac cactttgtga
     1441 tcgacggaga tggtacaggc aagccttatg agggaaaaca gaccatggat cttgaagtca
     1501 aagagggcgg acctctgcct tttgcctttg atatcctgac cactgcattc cattacggca
     1561 acagggtatt cgtgaaatat ccagacaaca tacaagacta ttttaagcag tcgtttccta
     1621 aggggtattc gtgggaacga agcttgactt tcgaagacgg gggcatttgc aatgccagaa
     1681 acgacataac aatggaaggg gacactttct ataataaagt tcgattttat ggtaccaact
     1741 ttcccgccaa tggtccagtt atgcagaaga agacgctgaa atgggagccc tccactgaga
     1801 aaatgtatgt gcgtgatgga gtgctgacgg gtgatattga gatggctttg ttgcttgaag
     1861 gaaatgccca ttaccgatgt gacttcagaa ctacttacaa agctaaggag aagggtgtca
     1921 agttaccagg cgcccacttt gtggaccacg ccattgagat tttaagccat gacaaagatt
     1981 acaacaaggt taagctgtat gagcatgctg ttgctcattc tggattgcct gacaatgcca
     2041 gacgataagc ggccgcgact ctagatcata atcagccata ccacatt
//
