LOCUS       sequence-88652-e        2790 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nuclear Envelope, Excitation: 505 / 569, Emission: 516
            / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2790)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 2790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEos4b-LaminA-C-18
FEATURES             Location/Qualifiers
     source          1..2790
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      19..28
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             25..705
                     /codon_start=1
                     /product="fixation-resistant truly monomeric green-to-red 
                     photoswitchable fluorescent protein (Paez-Segala et al., 
                     2015)"
                     /label=mEos4b
                     /note="derived from mEos2"
                     /translation="MVSAIKPDMRIKLRMEGNVNGHHFVIDGDGTGKPYEGKQTMDLEV
                     KEGGPLPFAFDILTTAFHYGNRVFVKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNA
                     RNDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIEMALL
                     LEGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHAIEILSHDKDYNKVKLYEHAVAHSGLP
                     DNARR"
ORIGIN
        1 ccgctagcgc taccggtcgc caccatggtg agtgcgatta agccagacat gaggatcaaa
       61 ctccgtatgg aaggcaacgt aaacgggcac cactttgtga tcgacggaga tggtacaggc
      121 aagccttatg agggaaaaca gaccatggat cttgaagtca aagagggcgg acctctgcct
      181 tttgcctttg atatcctgac cactgcattc cattacggca acagggtatt cgtgaaatat
      241 ccagacaaca tacaagacta ttttaagcag tcgtttccta aggggtattc gtgggaacga
      301 agcttgactt tcgaagacgg gggcatttgc aatgccagaa acgacataac aatggaaggg
      361 gacactttct ataataaagt tcgattttat ggtaccaact ttcccgccaa tggtccagtt
      421 atgcagaaga agacgctgaa atgggagccc tccactgaga aaatgtatgt gcgtgatgga
      481 gtgctgacgg gtgatattga gatggctttg ttgcttgaag gaaatgccca ttaccgatgt
      541 gacttcagaa ctacttacaa agctaaggag aagggtgtca agttaccagg cgcccacttt
      601 gtggaccacg ccattgagat tttaagccat gacaaagatt acaacaaggt taagctgtat
      661 gagcatgctg ttgctcattc tggattgcct gacaatgcca gacgatccgg actcagatct
      721 ggcagcggtg gaggcagcgc atccggcgga agcggaagcg agaccccgtc ccagcggcgc
      781 gccacccgca gcggggcgca ggccagctcc actccgctgt cgcccacccg catcacccgg
      841 ctgcaggaga aggaggacct gcaggagctc aatgatcgct tggcggtcta catcgaccgt
      901 gtgcgctcgc tggaaacgga gaacgcaggg ctgcgccttc gcatcaccga gtctgaagag
      961 gtggtcagcc gcgaggtgtc cggcatcaag gccgcctacg aggccgagct cggggatgcc
     1021 cgcaagaccc ttgactcagt agccaaggag cgcgcccgcc tgcagctgga gctgagcaaa
     1081 gtgcgtgagg agtttaagga gctgaaagcg cgcaatacca agaaggaggg tgacctgata
     1141 gctgctcagg ctcggctgaa ggacctggag gctctgctga actccaagga ggccgcactg
     1201 agcactgctc tcagtgagaa gcgcacgctg gagggcgagc tgcatgatct gcggggccag
     1261 gtggccaagc ttgaggcagc cctaggtgag gccaagaagc aacttcagga tgagatgctg
     1321 cggcgggtgg atgctgagaa caggctgcag accatgaagg aggaactgga cttccagaag
     1381 aacatctaca gtgaggagct gcgtgagacc aagcgccgtc atgagacccg actggtggag
     1441 attgacaatg ggaagcagcg tgagtttgag agccggctgg cggatgcgct gcaggaactg
     1501 cgggcccagc atgaggacca ggtggagcag tataagaagg agctggagaa gacttattct
     1561 gccaagctgg acaatgccag gcagtctgct gagaggaaca gcaacctggt gggggctgcc
     1621 cacgaggagc tgcagcagtc gcgcatccgc atcgacagcc tctctgccca gctcagccag
     1681 ctccagaagc agctggcagc caaggaggcg aagcttcgag acctggagga ctcactggcc
     1741 cgtgagcggg acaccagccg gcggctgctg gcggaaaagg agcgggagat ggccgagatg
     1801 cgggcaagga tgcagcagca gctggacgag taccaggagc ttctggacat caagctggcc
     1861 ctggacatgg agatccacgc ctaccgcaag ctcttggagg gcgaggagga gaggctacgc
     1921 ctgtccccca gccctacctc gcagcgcagc cgtggccgtg cttcctctca ctcatcccag
     1981 acacagggtg ggggcagcgt caccaaaaag cgcaaactgg agtccactga gagccgcagc
     2041 agcttctcac agcacgcacg cactagcggg cgcgtggccg tggaggaggt ggatgaggag
     2101 ggcaagtttg tccggctgcg caacaagtcc aatgaggacc agtccatggg caattggcag
     2161 atcaagcgcc agaatggaga tgatcccttg ctgacttacc ggttcccacc aaagttcacc
     2221 ctgaaggctg ggcaggtggt gacgatctgg gctgcaggag ctggggccac ccacagcccc
     2281 cctaccgacc tggtgtggaa ggcacagaac acctggggct gcgggaacag cctgcgtacg
     2341 gctctcatca actccactgg ggaagaagtg gccatgcgca agctggtgcg ctcagtgact
     2401 gtggttgagg acgacgagga tgaggatgga gatgacctgc tccatcacca ccacggctcc
     2461 cactgcagca gctcggggga ccccgctgag tacaacctgc gctcgcgcac cgtgctgtgc
     2521 gggacctgcg ggcagcctgc cgacaaggca tctgccagcg gctcaggagc ccaggtgggc
     2581 ggacccatct cctctggctc ttctgcctcc agtgtcacgg tcactcgcag ctaccgcagt
     2641 gtggggggca gtgggggtgg cagcttcggg gacaatctgg tcacccgctc ctacctcctg
     2701 ggcaactcca gcccccgaac ccagagcccc cagaactgca gcatcatgta aggatccacc
     2761 ggatctagat aactgatcat aatcagccat
//
