LOCUS       sequence-88653-e        2052 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Lysosome Membrane, Excitation: 505 / 569, Emission: 
            516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2052)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEos4b-Lysosomes-20
FEATURES             Location/Qualifiers
     source          1..2052
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      37..46
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      41..50
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(43..105,106..1263)
                     /codon_start=1
                     /product="rat lysosome-associated membrane glycoprotein 1"
                     /label=LAMP1
                     /translation="MAAPGARRPLLLLLLAGLAHSAPALFEVKDNNGTACIMASFSASF
                     LTTYEAGHVSKVSNMTLPASAEVLKNSSSCGEKNASEPTLAITFGEGYLLKLTFTKNTT
                     RYSVQHMYFTYNLSDTQFFPNASSKGPDTVDSTTDIKADINKTYRCVSDIRVYMKNVTI
                     VLWDATIQAYLPSSNFSKEETRCPQDQPSPTTGPPSPSPPLVPTNPSVSKYNVTGDNGT
                     CLLASMALQLNITYMKKDNTTVTRAFNINPSDKYSGTCGAQLVTLKVGNKSRVLELQFG
                     MNATSSLFFLQGVQLNMTLPDAIEPTFSTSNYSLKALQASVGNSYKCNSEEHIFVSKAL
                     ALNVFSVQVQAFRVESDRFGSVEECVQDGNNMLIPIAVGGALAGLVLIVLIAYLIGRKR
                     SHAGYQTI"
     regulatory      1318..1327
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1324..2007
                     /codon_start=1
                     /product="fixation-resistant truly monomeric green-to-red 
                     photoswitchable fluorescent protein (Paez-Segala et al., 
                     2015)"
                     /label=mEos4b
                     /note="derived from mEos2"
                     /translation="MVSAIKPDMRIKLRMEGNVNGHHFVIDGDGTGKPYEGKQTMDLEV
                     KEGGPLPFAFDILTTAFHYGNRVFVKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNA
                     RNDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIEMALL
                     LEGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHAIEILSHDKDYNKVKLYEHAVAHSGLP
                     DNARR"
ORIGIN
        1 gagatccgct agcgctaccg gactcagatc tcgagcgcca ccatggcggc cccgggcgcc
       61 cggcgtccgc tgctcctgtt gctgctggca ggccttgcac acagcgcccc agcactgttc
      121 gaggtgaaag acaacaacgg cacagcgtgt ataatggcca gcttctctgc ctcctttctg
      181 accacctatg aggctggaca tgtttctaag gtctcgaata tgaccctgcc agcctctgca
      241 gaagtcctga agaatagcag ctcttgtggt gaaaagaatg cttctgagcc caccctcgca
      301 atcacctttg gagaaggata tttactgaaa ctcaccttca caaaaaacac aacacgttac
      361 agtgtccagc acatgtattt cacatataac ctgtcagaca cacaattctt tcccaatgcc
      421 agctccaaag ggcccgacac tgtggattcc acaactgaca tcaaggcaga catcaacaaa
      481 acataccgat gtgtcagcga catcagggtc tacatgaaga atgtgaccat tgtgctctgg
      541 gacgctacta tccaggccta cctgccgagt agcaacttca gcaaggaaga gacacgctgc
      601 ccacaggatc aaccttcccc aactactggg ccacccagcc cctcaccacc acttgtgccc
      661 acaaacccca gtgtgtccaa gtacaatgtg actggtgaca atggaacctg cctgctggcc
      721 tctatggcac tgcaactcaa catcacctac atgaagaagg acaacacgac tgtgaccaga
      781 gcattcaaca tcaacccaag tgacaaatat agtgggactt gcggtgccca gttggtgacc
      841 ctgaaggtgg ggaacaagag cagagtcctg gagctgcagt ttgggatgaa tgccacttct
      901 agcctgtttt tcctgcaagg agttcagttg aacatgactc ttcctgatgc catagagccc
      961 acgttcagca cctccaacta ttccctgaaa gctcttcagg ccagtgtcgg caactcatac
     1021 aagtgcaaca gtgaggagca catctttgtc agcaaggcgc tcgccctcaa tgtcttcagc
     1081 gtgcaagtcc aggctttcag ggtagaaagt gacaggtttg ggtctgtgga agagtgtgta
     1141 caggacggta acaacatgct gatccccatt gctgtgggcg gggccctggc agggctggtc
     1201 ctcatcgtcc tcatcgccta cctcatcggc aggaagagga gtcacgcggg ctatcagacc
     1261 atctcggaat tcggctccac cggctccacc ggctccaccg gcgcggatcc accggtcgcc
     1321 accatggtga gtgcgattaa gccagacatg aggatcaaac tccgtatgga aggcaacgta
     1381 aacgggcacc actttgtgat cgacggagat ggtacaggca agccttatga gggaaaacag
     1441 accatggatc ttgaagtcaa agagggcgga cctctgcctt ttgcctttga tatcctgacc
     1501 actgcattcc attacggcaa cagggtattc gtgaaatatc cagacaacat acaagactat
     1561 tttaagcagt cgtttcctaa ggggtattcg tgggaacgaa gcttgacttt cgaagacggg
     1621 ggcatttgca atgccagaaa cgacataaca atggaagggg acactttcta taataaagtt
     1681 cgattttatg gtaccaactt tcccgccaat ggtccagtta tgcagaagaa gacgctgaaa
     1741 tgggagccct ccactgagaa aatgtatgtg cgtgatggag tgctgacggg tgatattgag
     1801 atggctttgt tgcttgaagg aaatgcccat taccgatgtg acttcagaac tacttacaaa
     1861 gctaaggaga agggtgtcaa gttaccaggc gcccactttg tggaccacgc cattgagatt
     1921 ttaagccatg acaaagatta caacaaggtt aagctgtatg agcatgctgt tgctcattct
     1981 ggattgcctg acaatgccag acgataagcg gccgcgactc tagatcataa tcagccatac
     2041 caacaatttg ta
//
