LOCUS       sequence-88654-e        1105 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Mannosidase/Golgi, Excitation: 505 / 569, Emission: 
            516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1105)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 1105)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEos4b-MannII-N-10
FEATURES             Location/Qualifiers
     source          1..1105
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      383..392
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             389..1072
                     /codon_start=1
                     /product="fixation-resistant truly monomeric green-to-red 
                     photoswitchable fluorescent protein (Paez-Segala et al., 
                     2015)"
                     /label=mEos4b
                     /note="derived from mEos2"
                     /translation="MVSAIKPDMRIKLRMEGNVNGHHFVIDGDGTGKPYEGKQTMDLEV
                     KEGGPLPFAFDILTTAFHYGNRVFVKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNA
                     RNDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIEMALL
                     LEGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHAIEILSHDKDYNKVKLYEHAVAHSGLP
                     DNARR"
ORIGIN
        1 cgtcagatcc gctagcgcca ccatgaagtt aagtcgccag ttcaccgtgt ttggcagcgc
       61 gatcttctgc gtcgtaatct tctcactcta cctgatgctg gacaggggtc acttggacta
      121 ccctcggggc ccgcgccagg agggctcctt tccgcagggc cagctttcaa tattgcaaga
      181 aaagattgac catttggagc gtttgctcgc tgagaacaac gagatcatct caaatatcag
      241 agactcagtc atcaacctga gcgagtctgt ggaggacggc ccgcgggggt caccaggcaa
      301 cgccagccaa ggctccatcc acctccactc gccacagttg gccctgcagg ctgaccccag
      361 ctccggatcg gatccaccgg tcgccaccat ggtgagtgcg attaagccag acatgaggat
      421 caaactccgt atggaaggca acgtaaacgg gcaccacttt gtgatcgacg gagatggtac
      481 aggcaagcct tatgagggaa aacagaccat ggatcttgaa gtcaaagagg gcggacctct
      541 gccttttgcc tttgatatcc tgaccactgc attccattac ggcaacaggg tattcgtgaa
      601 atatccagac aacatacaag actattttaa gcagtcgttt cctaaggggt attcgtggga
      661 acgaagcttg actttcgaag acgggggcat ttgcaatgcc agaaacgaca taacaatgga
      721 aggggacact ttctataata aagttcgatt ttatggtacc aactttcccg ccaatggtcc
      781 agttatgcag aagaagacgc tgaaatggga gccctccact gagaaaatgt atgtgcgtga
      841 tggagtgctg acgggtgata ttgagatggc tttgttgctt gaaggaaatg cccattaccg
      901 atgtgacttc agaactactt acaaagctaa ggagaagggt gtcaagttac caggcgccca
      961 ctttgtggac cacgccattg agattttaag ccatgacaaa gattacaaca aggttaagct
     1021 gtatgagcat gctgttgctc attctggatt gcctgacaat gccagacgat aagcggccgc
     1081 gactctagat cataatcagc catac
//
