LOCUS       sequence-88655-e        6719 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytoskeleton, Excitation: 505 / 569, Emission: 516 / 
            581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6719)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 6719)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEos4b-MyosinIIA-C-18
FEATURES             Location/Qualifiers
     source          1..6719
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      35..44
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             41..721
                     /codon_start=1
                     /product="fixation-resistant truly monomeric green-to-red 
                     photoswitchable fluorescent protein (Paez-Segala et al., 
                     2015)"
                     /label=mEos4b
                     /note="derived from mEos2"
                     /translation="MVSAIKPDMRIKLRMEGNVNGHHFVIDGDGTGKPYEGKQTMDLEV
                     KEGGPLPFAFDILTTAFHYGNRVFVKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNA
                     RNDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIEMALL
                     LEGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHAIEILSHDKDYNKVKLYEHAVAHSGLP
                     DNARR"
ORIGIN
        1 gttgacgcct cgagcccgct tagcgctacc ggtcgccacc atggtgagtg cgattaagcc
       61 agacatgagg atcaaactcc gtatggaagg caacgtaaac gggcaccact ttgtgatcga
      121 cggagatggt acaggcaagc cttatgaggg aaaacagacc atggatcttg aagtcaaaga
      181 gggcggacct ctgccttttg cctttgatat cctgaccact gcattccatt acggcaacag
      241 ggtattcgtg aaatatccag acaacataca agactatttt aagcagtcgt ttcctaaggg
      301 gtattcgtgg gaacgaagct tgactttcga agacgggggc atttgcaatg ccagaaacga
      361 cataacaatg gaaggggaca ctttctataa taaagttcga ttttatggta ccaactttcc
      421 cgccaatggt ccagttatgc agaagaagac gctgaaatgg gagccctcca ctgagaaaat
      481 gtatgtgcgt gatggagtgc tgacgggtga tattgagatg gctttgttgc ttgaaggaaa
      541 tgcccattac cgatgtgact tcagaactac ttacaaagct aaggagaagg gtgtcaagtt
      601 accaggcgcc cactttgtgg accacgccat tgagatttta agccatgaca aagattacaa
      661 caaggttaag ctgtatgagc atgctgttgc tcattctgga ttgcctgaca atgccagacg
      721 atccggactc agatctggca gcggtggagg cagcgcatcc ggcggaagcg gaagcgctca
      781 gcaggctgca gacaagtacc tctatgtgga taaaaacttc atcaataacc cgctggccca
      841 agctgactgg gctgccaaga agttggtatg ggtgccttcc agcaagaatg gctttgaacc
      901 agccagcctc aaggaggagg tgggagaaga ggccattgta gagctggtag agaatgggaa
      961 gaaggtgaag gtgaacaagg acgacatcca gaagatgaac ccacccaagt tctccaaggt
     1021 ggaggacatg gcagagctca cgtgcctcaa cgaagcttcg gtgctgcaca acctcaagga
     1081 gcgatactac tcagggctta tctacaccta ttcaggcctg ttctgtgtgg tcatcaaccc
     1141 ttataagaac ctgcccatct actcagagga gatcgtggag atgtacaagg gcaagaagag
     1201 gcacgagatg ccaccccaca tctacgccat cacagatact gcctaccgga gcatgatgca
     1261 ggaccgggaa gatcagtcca tcctgtgcac gggggagtct ggagcaggga agacagagaa
     1321 caccaagaaa gtcatccagt acctggcaca tgtggcctcc tcacacaaga gcaagaagga
     1381 ccagggggag ttggagcggc agctgctaca ggccaaccct atcctagagg cctttggaaa
     1441 cgccaagacg gtgaagaatg acaactcctc tcgattcggt aaattcattc gtatcaactt
     1501 tgatgtcaat ggctacattg ttggtgccaa cattgagact tatcttctgg agaaatctcg
     1561 tgctatccgc caagccaaag aggagcggac cttccacatc ttctactacc tgctgtctgg
     1621 ggccggagaa cacctgaaga ctgatctcct gttggagcca tacaacaaat accgcttcct
     1681 gtccaacggg cacgtcacca tccctgggca gcaggacaag gacatgttcc aggagacaat
     1741 ggaggccatg agaattatgg gtatcccaga ggatgagcag atgggcttgc tgcgggtcat
     1801 ctctggggtc cttcagcttg gcaacattgc cttcaagaag gagcggaaca ctgaccaggc
     1861 gtccatgccg gacaacacag ctgctcaaaa ggtgtcccac ctcctgggga tcaatgtgac
     1921 cgacttcacc agaggcatcc tcaccccacg catcaaggtg ggcagagact atgtgcagaa
     1981 ggcgcagact aaagagcagg ctgactttgc cattgaggcc ttggccaagg ctacctatga
     2041 gcggatgttc cgctggctgg tgcttcgcat caacaaagct ctggacaaga ccaagaggca
     2101 gggcgcctca tttatcggga tactggacat cgctggcttt gaaatctttg atctgaactc
     2161 cttcgagcag ctgtgcatca actacaccaa cgagaagctg cagcagctgt tcaaccacac
     2221 catgttcatc ctggagcagg aggagtacca gcgagagggc atcgagtgga acttcatcga
     2281 cttcggcctg gacctgcagc cctgcatcga cctcattgag aagccggcgg gtcccccagg
     2341 catcctggcc ctgctagatg aggagtgctg gtttcctaag gccactgaca agagcttcgt
     2401 ggagaaggtg gtgcaggagc agggcaccca ccccaagttc cagaagccca agcaactgaa
     2461 ggacaaggct gatttctgca ttatccacta tgccggcaag gtggactata aagctgacga
     2521 gtggctgatg aagaacatgg accccttgaa cgacaacatc gccacgctgc ttcaccagtc
     2581 ctcagacaag tttgtctctg agctgtggaa ggatgtggat cggatcattg gcttggacca
     2641 agtggctgga atgtccgaga cagcactacc tggtgccttc aagacccgga agggcatgtt
     2701 ccgtactgtc ggacagctgt acaaggagca gctggccaag ctcatggcca cgttgaggaa
     2761 taccaacccc aacttcgtgc gctgcatcat tcccaaccat gagaagaagg ccggcaaact
     2821 ggacccgcac ttggtgctgg accagctgcg ctgcaatggc gtccttgagg gcatccggat
     2881 ctgccgccag ggctttccca acagggtggt cttccaggag ttccggcaga ggtatgagat
     2941 cctcaccccc aactccatcc cgaagggctt catggatggc aagcaagcgt gtgtgctcat
     3001 gatcaaagcc ttggagcttg acagcaacct gtaccgcatc ggccagagca aagtgttctt
     3061 ccgggcagga gtgctcgccc acctggagga agagcgggac ctgaagatca ccgatgtcat
     3121 cattggcttc caggcctgct gcaggggcta cctggccagg aaggcctttg ccaagaggca
     3181 gcaacagctg accgccatga aggtcctaca gaggaactgt gctgcgtacc tcaggctgcg
     3241 caactggcag tggtggaggc tcttcaccaa ggtcaagccc ctgttgaact caataagaca
     3301 tgaggatgag ctgttagcca aggaggcgga actgacaaag gttcgagaga aacatctggc
     3361 tgcagagaac aggctgacag agatggagac gatgcagtct cagctcatgg cagagaagct
     3421 gcagcttcag gagcagctgc aggcggagac agagctgtgt gccgaggctg aggagctccg
     3481 ggcccgtctg acagcgaaga agcaggagct ggaggagata tgccatgacc tggaggccag
     3541 ggtggaggag gaggaggagc gctgccagta cctgcaggcc gagaagaaga agatgcagca
     3601 gaacatccag gaacttgagg agcagttgga ggaggaggag agcgcccggc agaagctgca
     3661 gcttgagaag gtgaccaccg aggccaagct gaagaaactg gaggaggacc agatcatcat
     3721 ggaggaccag aactgcaaac tggccaagga gaagaaactg ctggaagaca gagtagctga
     3781 gttcactacc aacctcatgg aagaggagga gaagtccaag agcctggcca agctcaagaa
     3841 caagcacgag gcaatgatca ccgacctgga agagcgcctc cgtagggagg agaagcagag
     3901 gcaggagttg gagaagaccc gtcgcaagct ggagggagac tccacagacc tcagtgacca
     3961 gattgctgag ctccaggcgc agatagcaga gctcaagatg cagctggcca agaaggagga
     4021 ggagttgcag gctgccttgg ccagagtgga agaagaagct gctcagaaga atatggccct
     4081 gaagaagatc cgagaactgg aaactcagat atctgagctc caggaggacc tggagtcgga
     4141 gcgagcctcc aggaataaag ccgagaagca gaaacgggat ctgggagagg agctggaggc
     4201 gctgaagaca gagctggagg acacgctgga ctccacggct gcccagcagg agctgaggtc
     4261 gaagcgtgag caggaggtga gcatcctgaa gaagactctg gaggacgagg ccaagaccca
     4321 tgaggcccag atccaggaga tgaggcagaa gcactcacag gctgtggagg agctggcaga
     4381 tcagttggag cagacgaagc gggtaaaagc tacccttgag aaggcgaagc agaccctgga
     4441 gaatgagcgg ggagagctgg ccaatgaggt gaaggccctg ctgcaaggca agggcgactc
     4501 agagcacaag cgcaagaagg tggaggcgca gctgcaagaa ctgcaggtca agttcagcga
     4561 gggagagcgc gtgcgaaccg aactggccga caaggtcacc aagctgcagg ttgaactgga
     4621 cagtgtgacc ggtctcctta gccagtctga cagcaagtcc agcaagctta cgaaggactt
     4681 ctctgcgctg gagtcccagc ttcaggacac acaggagttg ctccaggagg agaaccggca
     4741 gaagctgagc ctgagcacca agctcaagca gatggaggat gagaaaaact ccttcaggga
     4801 gcagctggag gaggaggagg aggccaagcg caacttggag aagcagatcg ccacgctcca
     4861 tgcccaggtg accgacatga agaagaagat ggaggacggt gtagggtgcc tggagactgc
     4921 agaggaggcg aagcggaggc ttcagaagga cttggaaggc ctgagccagc ggcttgagga
     4981 gaaggtggct gcctacgata agctggagaa gaccaagaca cggctgcagc aggagctgga
     5041 cgacctgctg gttgacctgg accaccagcg gcagagcgtc tccaacctgg aaaagaagca
     5101 gaagaagttc gaccagctcc tagccgagga gaagaccatc tcggccaagt atgcagagga
     5161 gcgtgaccga gctgaggctg aggcccgtga gaaggagaca aaggcgctat cactggcccg
     5221 ggcgcttgag gaggccatgg agcagaaggc agagctggag cggctcaaca agcagttccg
     5281 cacggagatg gaggacctca tgagctccaa ggatgacgtg ggcaagagtg tccacgagct
     5341 ggagaagtcc aagcgggcct tggagcagca ggtggaggag atgaagaccc agctggagga
     5401 gctggaggat gagctgcagg ccacggagga tgccaagctc cgcctggagg tgaacctgca
     5461 ggccatgaag gcccagtttg agcgggatct gcagggccgg gatgaacaga gcgaggagaa
     5521 gaagaagcag ctggtcagac aggtgcggga gatggaggcg gagctggagg atgagaggaa
     5581 gcagcgctcc atggccatgg ccgcacgcaa gaaactggag atggatctga aggacctgga
     5641 ggcacacatt gacacagcca ataagaaccg ggaagaggcc atcaaacagc tgcggaagct
     5701 tcaggcccag atgaaggact gcatgcggga gctggacgac acgcgcgcct cccgggagga
     5761 gatcctggcg caggccaagg agaatgagaa gaagctgaag agcatggagg ccgagatgat
     5821 tcagctgcag gaggaactgg cagctgctga gcgtgctaag cgtcaggccc aacaggaacg
     5881 ggacgagctg gctgatgaga tcgccaacag cagtggcaaa ggggccctag cattagagga
     5941 gaagcggcga ctggaggccc gcattgccct gctggaggag gagctggagg aggaacaggg
     6001 caacacggag ctgatcaacg atcggctgaa gaaggccaac ctgcagatcg accaaataaa
     6061 caccgacctg aacctggaac gcagccacgc acagaagaat gagaatgcgc gacagcagct
     6121 ggaacgccag aacaaggagc tcaaggccaa gctgcaggaa atggagagtg ctgtcaagtc
     6181 caaatacaag gcctccatcg cggccttgga ggccaaaatt gcacagctgg aggaacagct
     6241 ggacaacgag accaaggagc gccaggcagc ctccaagcag gtgcgccgga cggagaagaa
     6301 gctgaaggac gtgctgctgc aggtggagga cgagcggagg aacgcggaac agttcaagga
     6361 ccaggctgac aaggcgtcca cccgcctgaa gcagcttaaa cggcagctag aggaggctga
     6421 agaggaggcc cagcgggcca atgcctcacg ccggaagctg cagcgtgagc tggaagatgc
     6481 cacagagacc gctgatgcta tgaaccgcga ggtcagctcc ctgaagaaca aactgaggcg
     6541 tggggacctg ccatttgtcg tgactcgccg aattgttcgg aaaggcactg gcgactgctc
     6601 agacgaggag gtcgacggta aagcagatgg ggccgatgcc aaggcagctg aataggaatt
     6661 ctgcagtcga cggtaccgcg ggcccgggat ccaccggatc tagataactg atcataatc
//
