LOCUS       sequence-88656-e        2320 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytoskeleton, Excitation: 505 / 569, Emission: 516 / 
            581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2320)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEos4b-Myotilin-C-14
FEATURES             Location/Qualifiers
     source          1..2320
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      40..49
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             46..726
                     /codon_start=1
                     /product="fixation-resistant truly monomeric green-to-red 
                     photoswitchable fluorescent protein (Paez-Segala et al., 
                     2015)"
                     /label=mEos4b
                     /note="derived from mEos2"
                     /translation="MVSAIKPDMRIKLRMEGNVNGHHFVIDGDGTGKPYEGKQTMDLEV
                     KEGGPLPFAFDILTTAFHYGNRVFVKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNA
                     RNDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIEMALL
                     LEGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHAIEILSHDKDYNKVKLYEHAVAHSGLP
                     DNARR"
ORIGIN
        1 ccattgctag tgacgtcaga tccgctagcg ctaccggtcg ccaccatggt gagtgcgatt
       61 aagccagaca tgaggatcaa actccgtatg gaaggcaacg taaacgggca ccactttgtg
      121 atcgacggag atggtacagg caagccttat gagggaaaac agaccatgga tcttgaagtc
      181 aaagagggcg gacctctgcc ttttgccttt gatatcctga ccactgcatt ccattacggc
      241 aacagggtat tcgtgaaata tccagacaac atacaagact attttaagca gtcgtttcct
      301 aaggggtatt cgtgggaacg aagcttgact ttcgaagacg ggggcatttg caatgccaga
      361 aacgacataa caatggaagg ggacactttc tataataaag ttcgatttta tggtaccaac
      421 tttcccgcca atggtccagt tatgcagaag aagacgctga aatgggagcc ctccactgag
      481 aaaatgtatg tgcgtgatgg agtgctgacg ggtgatattg agatggcttt gttgcttgaa
      541 ggaaatgccc attaccgatg tgacttcaga actacttaca aagctaagga gaagggtgtc
      601 aagttaccag gcgcccactt tgtggaccac gccattgaga ttttaagcca tgacaaagat
      661 tacaacaagg ttaagctgta tgagcatgct gttgctcatt ctggattgcc tgacaatgcc
      721 agacgatccg gaagtgctgg gagcgcaagc ggaagcggag gctccggttt taactacgaa
      781 cgtccaaaac acttcatcca gtcccaaaac ccatgtggct ccagattgca gcctcctgga
      841 ccagaaacct ccagcttctc tagccagacc aaacagtctt ccattatcat ccagccccgc
      901 cagtgtacag agcaaagatt ttctgcctcc tcaacactga gctctcacat caccatgtcc
      961 tcctctgctt tccctgcttc tccccagcag catgctggct ccaacccagg ccaaagggtt
     1021 acaaccacct ataaccagtc cccagccagc ttcctcagct ccatattacc atcacagcct
     1081 gattacaata gcagtaaaat cccttccgct atggattcca actatcaaca gtcctcagct
     1141 ggccaaccta taaatgcaaa gccatcccaa actgcaaatg ctaagcccat accaagaact
     1201 cctgatcatg aaatacaagg atcaaaagaa gctttgattc aagatttgga aagaaagctg
     1261 aaatgcaagg acacccttct tcataatgga aatcaacgtc taacatatga agagaagatg
     1321 gctcgcagat tgctaggacc acagaatgca gctgctgtgt ttcaagctca ggatgacagt
     1381 ggtgcacaag actcgcagca acacaactca gaacatgcgc gactgcaagt tcctacatca
     1441 caagtaagaa gtagatcaac ctcaagggga gatgtgaatg atcaggatgc aatccaggag
     1501 aaattttacc caccacgttt cattcaagtg ccagagaaca tgtcgattga tgaaggaaga
     1561 ttctgcagaa tggacttcaa agtgagtgga ctgccagctc ctgatgtgtc atggtatcta
     1621 aatggaagaa cagttcaatc agatgatttg cacaaaatga tagtgtctga gaagggtctt
     1681 cattcactca tctttgaagt agtcagagct tcagatgcag gggcttatgc atgtgttgcc
     1741 aagaatagag caggagaagc caccttcact gtgcagctgg atgtccttgc aaaagaacat
     1801 aaaagagcac caatgtttat ctacaaacca cagagcaaaa aagttttaga gggagattca
     1861 gtgaaactag aatgccagat ctcggctata cctccaccaa agcttttctg gaaaagaaat
     1921 aatgaaatgg tacaattcaa cactgaccga ataagcttat atcaagataa cactggaaga
     1981 gttactttac tgataaaaga tgtaaacaag aaagatgctg ggtggtatac tgtgtcagca
     2041 gttaatgaag ctggagtgac tacatgtaac acaagattag acgttacggc acgtccaaac
     2101 caaactcttc cagctcctaa gcagttacgg gttcgaccaa cattcagcaa atatttagca
     2161 cttaatggga aaggtttgaa tgtaaaacaa gcttttaacc cagaaggaga atttcagcgt
     2221 ttggcagctc aatctggact ctatgaaagt gaagaacttt aaggatccac cggatctaga
     2281 taactgatca taatcagcca taccacatgt agaagatgcc
//
