LOCUS       sequence-88658-e        1976 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Mitochondrial Matrix, Excitation: 505 / 569, Emission:
            516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1976)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 1976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEos4b-PDHA1-N-10
FEATURES             Location/Qualifiers
     source          1..1976
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1238..1247
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1244..1927
                     /codon_start=1
                     /product="fixation-resistant truly monomeric green-to-red 
                     photoswitchable fluorescent protein (Paez-Segala et al., 
                     2015)"
                     /label=mEos4b
                     /note="derived from mEos2"
                     /translation="MVSAIKPDMRIKLRMEGNVNGHHFVIDGDGTGKPYEGKQTMDLEV
                     KEGGPLPFAFDILTTAFHYGNRVFVKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNA
                     RNDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIEMALL
                     LEGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHAIEILSHDKDYNKVKLYEHAVAHSGLP
                     DNARR"
ORIGIN
        1 ttgggcttgg ttagtgaccg tcagatccgc tagcaccgcc accatgagga agatgctcgc
       61 cgccgtctcc cgcgtgctgt ctggcgcttc tcagaagccg gcaagcagag tgctggtagc
      121 atcccgtaat tttgcaaatg atgctacatt tgaaattaag aaatgtgacc ttcaccggct
      181 ggaagaaggc cctcctgtca caacagtgct caccagggag gatgggctca aatactacag
      241 gatgatgcag actgtacgcc gaatggagtt gaaagcagat cagctgtata aacagaaaat
      301 tattcgtggt ttctgtcact tgtgtgatgg tcaggaagct tgctgtgtgg gcctggaggc
      361 cggcatcaac cccacagacc atctcatcac agcctaccgg gctcacggct ttactttcac
      421 ccggggcctt tccgtccgag aaattctcgc agagcttaca ggacgaaaag gaggttgtgc
      481 taaagggaaa ggaggatcga tgcacatgta tgccaagaac ttctacgggg gcaatggcat
      541 cgtgggagcg caggtgcccc tgggcgctgg gattgctcta gcctgtaagt ataatggaaa
      601 agatgaggtc tgcctgactt tatatggcga tggtgctgct aaccagggcc agatattcga
      661 agcttacaac atggcagctt tgtggaaatt accttgtatt ttcatctgtg agaataatcg
      721 ctatggaatg ggaacgtctg ttgagagagc ggcagccagc actgattact acaagagagg
      781 cgatttcatt cctgggctga gagtggatgg aatggatatc ctgtgcgtcc gagaggcaac
      841 aaggtttgct gctgcctatt gtagatctgg gaaggggccc atcctgatgg agctgcagac
      901 ttaccgttac cacggacaca gtatgagtga ccctggagtc agttaccgta cacgagaaga
      961 aattcaggaa gtaagaagta agagtgaccc tattatgctt ctcaaggaca ggatggtgaa
     1021 cagcaatctt gccagtgtgg aagaactaaa ggaaattgat gtggaagtga ggaaggagat
     1081 tgaggatgct gcccagtttg ccacggccga tcctgagcca cctttggaag agctgggcta
     1141 ccacatctac tccagcgacc caccttttga agttcgtggt gccaatcagt ggatcaagtt
     1201 taagtcagtc agtagctccg gaggggatcc accggtcgcc accatggtga gtgcgattaa
     1261 gccagacatg aggatcaaac tccgtatgga aggcaacgta aacgggcacc actttgtgat
     1321 cgacggagat ggtacaggca agccttatga gggaaaacag accatggatc ttgaagtcaa
     1381 agagggcgga cctctgcctt ttgcctttga tatcctgacc actgcattcc attacggcaa
     1441 cagggtattc gtgaaatatc cagacaacat acaagactat tttaagcagt cgtttcctaa
     1501 ggggtattcg tgggaacgaa gcttgacttt cgaagacggg ggcatttgca atgccagaaa
     1561 cgacataaca atggaagggg acactttcta taataaagtt cgattttatg gtaccaactt
     1621 tcccgccaat ggtccagtta tgcagaagaa gacgctgaaa tgggagccct ccactgagaa
     1681 aatgtatgtg cgtgatggag tgctgacggg tgatattgag atggctttgt tgcttgaagg
     1741 aaatgcccat taccgatgtg acttcagaac tacttacaaa gctaaggaga agggtgtcaa
     1801 gttaccaggc gcccactttg tggaccacgc cattgagatt ttaagccatg acaaagatta
     1861 caacaaggtt aagctgtatg agcatgctgt tgctcattct ggattgcctg acaatgccag
     1921 acgataagcg gccgcgactc tagatcataa tcagccatac cacattgtag agtgcc
//
