LOCUS       sequence-88659-e        1226 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Mitochondrial Membrane, Excitation: 505 / 569, 
            Emission: 516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1226)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 1226)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEos4b-TOMM20-N-10
FEATURES             Location/Qualifiers
     source          1..1226
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      28..37
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      493..502
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             499..1182
                     /codon_start=1
                     /product="fixation-resistant truly monomeric green-to-red 
                     photoswitchable fluorescent protein (Paez-Segala et al., 
                     2015)"
                     /label=mEos4b
                     /note="derived from mEos2"
                     /translation="MVSAIKPDMRIKLRMEGNVNGHHFVIDGDGTGKPYEGKQTMDLEV
                     KEGGPLPFAFDILTTAFHYGNRVFVKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNA
                     RNDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIEMALL
                     LEGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHAIEILSHDKDYNKVKLYEHAVAHSGLP
                     DNARR"
ORIGIN
        1 atgatttaac ggttcagatc cgctagcgcc accatggtgg gtcggaacag cgccatcgcc
       61 gccggtgtat gcggggccct tttcattggg tactgcatct acttcgaccg caaaagacga
      121 agtgacccca acttcaagaa caggcttcga gaacgaagaa agaaacagaa gcttgccaag
      181 gagagagctg ggctttccaa gttacctgac cttaaagatg ctgaagctgt tcagaagttc
      241 ttccttgaag aaatacagct tggtgaagag ttactagctc aaggtgaata tgagaagggc
      301 gtagaccatc tgacaaatgc aattgctgtg tgtggacagc cacagcagtt actgcaggtc
      361 ttacagcaaa ctcttccacc accagtgttc cagatgcttc tgactaagct cccaacaatt
      421 agtcagagaa ttgtaagtgc tcagagcttg gctgaagatg atgtggaagg cggtagcggg
      481 gatccaccgg tcgccaccat ggtgagtgcg attaagccag acatgaggat caaactccgt
      541 atggaaggca acgtaaacgg gcaccacttt gtgatcgacg gagatggtac aggcaagcct
      601 tatgagggaa aacagaccat ggatcttgaa gtcaaagagg gcggacctct gccttttgcc
      661 tttgatatcc tgaccactgc attccattac ggcaacaggg tattcgtgaa atatccagac
      721 aacatacaag actattttaa gcagtcgttt cctaaggggt attcgtggga acgaagcttg
      781 actttcgaag acgggggcat ttgcaatgcc agaaacgaca taacaatgga aggggacact
      841 ttctataata aagttcgatt ttatggtacc aactttcccg ccaatggtcc agttatgcag
      901 aagaagacgc tgaaatggga gccctccact gagaaaatgt atgtgcgtga tggagtgctg
      961 acgggtgata ttgagatggc tttgttgctt gaaggaaatg cccattaccg atgtgacttc
     1021 agaactactt acaaagctaa ggagaagggt gtcaagttac caggcgccca ctttgtggac
     1081 cacgccattg agattttaag ccatgacaaa gattacaaca aggttaagct gtatgagcat
     1141 gctgttgctc attctggatt gcctgacaat gccagacgat aagcggccgc gactctagat
     1201 cataatcagc catacccacc atttgt
//
