LOCUS       sequence-8866-ex        6862 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6862)
  AUTHORS   Liou J, Fivaz M, Inoue T, Meyer T
  TITLE     Live-cell imaging reveals sequential oligomerization and local 
            plasma membrane targeting of stromal interaction molecule 1 after 
            Ca2+ store depletion.
  JOURNAL   Proc Natl Acad Sci U S A. 2007 May 29. 104(22):9301-6.
  PUBMED    17517596
REFERENCE   2  (bases 1 to 6862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2007 May 29. 104(22):9301-6."
COMMENT     Sequence Label: pEX-CFP-STIM1-deltaK
FEATURES             Location/Qualifiers
     source          1..6862
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      678..687
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(684..686,687..689,690..1400)
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(729..750)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(990..1009)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1337..1358
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    1410..1434
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3433..3457)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3461..3526
                     /label=MCS
                     /note="multiple cloning site"
     polyA_signal    3650..3771
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3687..3706)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3741..3760
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3778..4233)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3915..3936)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4127..4146
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4260..4364
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4366..4723
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4366..4557
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4389..4409)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4574..4709
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4758..5552
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4758..5552
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4812..4831)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5422..5441
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5740..5759)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5784..5831
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6160..6748
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6649..6668
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcacca
      601 tggatgtatg cgtccgtctt gccctgtggc tcctctgggg actcctcctg caccagggcc
      661 agagcctcgc accggtcgcc accatggtga gcaagggcga ggagctgttc accggggtgg
      721 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg
      781 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca
      841 agctgcccgt gccctggccc accctcgtga ccaccctgac ctggggcgtg cagtgcttca
      901 gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
      961 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg
     1021 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
     1081 aggacggcaa catcctgggg cacaagctgg agtacaacta catcagccac aacgtctata
     1141 tcaccgccga caagcagaag aacggcatca aggccaactt caagatccgc cacaacatcg
     1201 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
     1261 ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca
     1321 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg
     1381 gcatggacga gctgtacaag tccggatcaa caagtttgta caaaaaagca ggctccctca
     1441 gccatagtca cagtgagaag gcgacaggaa ccagctcggg ggccaactct gaggagtcca
     1501 ctgcagcaga gttttgccga attgacaagc ccctgtgtca cagtgaggat gagaaactca
     1561 gcttcgaggc agtccgtaac atccacaaac tgatggacga tgatgccaat ggtgatgtgg
     1621 atgtggaaga aagtgatgag ttcctgaggg aagacctcaa ttaccatgac ccaacagtga
     1681 aacacagcac cttccatggt gaggataagc tcatcagcgt ggaggacctg tggaaggcat
     1741 ggaagtcatc agaagtatac aattggaccg tggatgaggt ggtacagtgg ctgatcacat
     1801 atgtggagct gcctcagtat gaggagacct tccggaagct gcagctcagt ggccatgcca
     1861 tgccaaggct ggctgtcacc aacaccacca tgacagggac tgtgctgaag atgacagacc
     1921 ggagtcatcg gcagaagctg cagctgaagg ctctggatac agtgctcttt gggcctcctc
     1981 tcttgactcg ccataatcac ctcaaggact tcatgctggt ggtgtctatc gttattggtg
     2041 tgggcggctg ctggtttgcc tatatccaga accgttactc caaggagcac atgaagaaga
     2101 tgatgaagga cttggagggg ttacaccgag ctgagcagag tctgcatgac cttcaggaaa
     2161 ggctgcacaa ggcccaggag gagcaccgca cagtggaggt ggagaaggtc catctggaaa
     2221 agaagctgcg cgatgagatc aaccttgcta agcaggaagc ccagcggctg aaggagctgc
     2281 gggagggtac tgagaatgag cggagccgcc aaaaatatgc tgaggaggag ttggagcagg
     2341 ttcgggaggc cttgaggaaa gcagagaagg agctagaatc tcacagctca tggtatgctc
     2401 cagaggccct tcagaagtgg ctgcagctga cacatgaggt ggaggtgcaa tattacaaca
     2461 tcaagaagca aaatgctgag aagcagctgc tggtggccaa ggagggggct gagaagataa
     2521 aaaagaagag aaacacactc tttggcacct tccacgtggc ccacagctct tccctggatg
     2581 atgtagatca taaaattcta acagctaagc aagcactgag cgaggtgaca gcagcattgc
     2641 gggagcgcct gcaccgctgg caacagatcg agatcctctg tggcttccag attgtcaaca
     2701 accctggcat ccactcactg gtggctgccc tcaacataga ccccagctgg atgggcagta
     2761 cacgccccaa ccctgctcac ttcatcatga ctgacgacgt ggatgacatg gatgaggaga
     2821 ttgtgtctcc cttgtccatg cagtccccta gcctgcagag cagtgttcgg cagcgcctga
     2881 cggagccaca gcatggcctg ggatctcaga gggatttgac ccattccgat tcggagtcct
     2941 ccctccacat gagtgaccgc cagcgtgtgg cccccaaacc tcctcagatg agccgtgctg
     3001 cagacgaggc tctcaatgcc atgacttcca atggcagcca ccggctgatc gagggggtcc
     3061 acccagggtc tctggtggag aaactgcctg acagccctgc cctggccaag aaggcattac
     3121 tggcgctgaa ccatgggctg gacaaggccc acagcctgat ggagctgagc ccctcagccc
     3181 cacctggtgg ctctccacat ttggattctt cccgttctca cagccccagc tccccagacc
     3241 cagacacacc atctccagtt ggggacagcc gagccctgca agccagccga aacacacgca
     3301 ttccccacct ggctggcaag aaggctgtgg ctgaggagga taatggctct attggcgagg
     3361 aaacagactc cagcccaggc tgacggaaga agtttcctct caaaatcttt aagaagcctc
     3421 ttaagaagta gaacccagct ttcttgtaca aagtggttga tccggactca gatctcgagc
     3481 tcaagcttcg aattctgcag tcgacggtac cgcgggcccg ggatccaccg gatctagata
     3541 actgatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc
     3601 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
     3661 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     3721 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt aacgcgtaaa
     3781 ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt
     3841 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag
     3901 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg
     3961 tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat
     4021 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc
     4081 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga
     4141 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac
     4201 ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg cacttttcgg ggaaatgtgc
     4261 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     4321 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtcctgag gcggaaagaa
     4381 ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
     4441 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
     4501 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
     4561 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     4621 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     4681 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaagatcgat caagagacag
     4741 gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
     4801 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     4861 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     4921 gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     4981 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     5041 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     5101 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     5161 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     5221 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     5281 aggcgagcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga
     5341 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     5401 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     5461 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     5521 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga
     5581 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
     5641 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     5701 catgctggag ttcttcgccc accctagggg gaggctaact gaaacacgga aggagacaat
     5761 accggaagga acccgcgcta tgacggcaat aaaaagacag aataaaacgc acggtgttgg
     5821 gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg cactctgtcg ataccccacc
     5881 gagaccccat tggggccaat acgcccgcgt ttcttccttt tccccacccc accccccaag
     5941 ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg ccatagcctc
     6001 aggttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     6061 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     6121 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     6181 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     6241 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     6301 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     6361 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     6421 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     6481 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     6541 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     6601 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     6661 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     6721 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     6781 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     6841 taaccgtatt accgccatgc at
//
