LOCUS       sequence-88660-e        2201 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Intermediate Filaments, Excitation: 505 / 569, 
            Emission: 516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2201)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEos4b-Vimentin-7
FEATURES             Location/Qualifiers
     source          1..2201
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1465..1474
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1471..2154
                     /codon_start=1
                     /product="fixation-resistant truly monomeric green-to-red 
                     photoswitchable fluorescent protein (Paez-Segala et al., 
                     2015)"
                     /label=mEos4b
                     /note="derived from mEos2"
                     /translation="MVSAIKPDMRIKLRMEGNVNGHHFVIDGDGTGKPYEGKQTMDLEV
                     KEGGPLPFAFDILTTAFHYGNRVFVKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNA
                     RNDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIEMALL
                     LEGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHAIEILSHDKDYNKVKLYEHAVAHSGLP
                     DNARR"
ORIGIN
        1 gatccgctag cgctaccgga ctcagatctc gagctcaagc ttcgaattcc catgtccacc
       61 aggtccgtgt cctcgtcctc ctaccgcagg atgttcggcg gcccgggcac cgcgagccgg
      121 ccgagctcca gccggagcta cgtgactacg tccacccgca cctacagcct gggcagcgcg
      181 ctgcgcccca gcaccagccg cagcctctac gcctcgtccc cgggcggcgt gtatgccacg
      241 cgctcctctg ccgtgcgcct gcggagcagc gtgcccgggg tgcggctcct gcaggactcg
      301 gtggacttct cgctggccga cgccatcaac accgagttca agaacacccg caccaacgag
      361 aaggtggagc tgcaggagct gaatgaccgc ttcgccaact acatcgacaa ggtgcgcttc
      421 ctggagcagc agaataagat cctgctggcc gagctcgagc agctcaaggg ccaaggcaag
      481 tcgcgcctgg gggacctcta cgaggaggag atgcgggagc tgcgccggca ggtggaccag
      541 ctaaccaacg acaaagcccg cgtcgaggtg gagcgcgaca acctggccga ggacatcatg
      601 cgcctccggg agaaattgca ggaggagatg cttcagagag aggaagccga aaacaccctg
      661 caatctttca gacaggatgt tgacaatgcg tctctggcac gtcttgacct tgaacgcaaa
      721 gtggaatctt tgcaagaaga gattgccttt ttgaagaaac tccacgaaga ggaaatccag
      781 gagctgcagg ctcagattca ggaacagcat gtccaaatcg atgtggatgt ttccaagcct
      841 gacctcacgg ctgccctgcg tgacgtacgt cagcaatatg aaagtgtggc tgccaagaac
      901 ctgcaggagg cagaagaatg gtacaaatcc aagtttgctg acctctctga ggctgccaac
      961 cggaacaatg acgccctgcg ccaggcaaag caggagtcca ctgagtaccg gagacaggtg
     1021 cagtccctca cctgtgaagt ggatgccctt aaaggaacca atgagtccct ggaacgccag
     1081 atgcgtgaaa tggaagagaa ctttgccgtt gaagctgcta actaccaaga cactattggc
     1141 cgcctgcagg atgagattca gaatatgaag gaggaaatgg ctcgtcacct tcgtgaatac
     1201 caagacctgc tcaatgttaa gatggccctt gacattgaga ttgccaccta caggaagctg
     1261 ctggaaggcg aggagagcag gatttctctg cctcttccaa acttttcctc cctgaacctg
     1321 agggaaacta atctggattc actccctctg gttgataccc actcaaaaag gacacttctg
     1381 attaagacgg ttgaaactag agatggacag gttatcaacg aaacttctca gcatcacgat
     1441 gaccttgaag gggatccacc ggtcgccacc atggtgagtg cgattaagcc agacatgagg
     1501 atcaaactcc gtatggaagg caacgtaaac gggcaccact ttgtgatcga cggagatggt
     1561 acaggcaagc cttatgaggg aaaacagacc atggatcttg aagtcaaaga gggcggacct
     1621 ctgccttttg cctttgatat cctgaccact gcattccatt acggcaacag ggtattcgtg
     1681 aaatatccag acaacataca agactatttt aagcagtcgt ttcctaaggg gtattcgtgg
     1741 gaacgaagct tgactttcga agacgggggc atttgcaatg ccagaaacga cataacaatg
     1801 gaaggggaca ctttctataa taaagttcga ttttatggta ccaactttcc cgccaatggt
     1861 ccagttatgc agaagaagac gctgaaatgg gagccctcca ctgagaaaat gtatgtgcgt
     1921 gatggagtgc tgacgggtga tattgagatg gctttgttgc ttgaaggaaa tgcccattac
     1981 cgatgtgact tcagaactac ttacaaagct aaggagaagg gtgtcaagtt accaggcgcc
     2041 cactttgtgg accacgccat tgagatttta agccatgaca aagattacaa caaggttaag
     2101 ctgtatgagc atgctgttgc tcattctgga ttgcctgaca atgccagacg ataagcggcc
     2161 gcgactctag atcataatca gcccataccc accattgtag g
//
