LOCUS       sequence-88661-e        2227 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Intermediate Filaments, Excitation: 505 / 569, 
            Emission: 516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2227)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 2227)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEos4b-Vimentin-C-18
FEATURES             Location/Qualifiers
     source          1..2227
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      28..37
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             34..714
                     /codon_start=1
                     /product="fixation-resistant truly monomeric green-to-red 
                     photoswitchable fluorescent protein (Paez-Segala et al., 
                     2015)"
                     /label=mEos4b
                     /note="derived from mEos2"
                     /translation="MVSAIKPDMRIKLRMEGNVNGHHFVIDGDGTGKPYEGKQTMDLEV
                     KEGGPLPFAFDILTTAFHYGNRVFVKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNA
                     RNDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIEMALL
                     LEGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHAIEILSHDKDYNKVKLYEHAVAHSGLP
                     DNARR"
ORIGIN
        1 acgtcagatc cgctagcgct accggtcgcc accatggtga gtgcgattaa gccagacatg
       61 aggatcaaac tccgtatgga aggcaacgta aacgggcacc actttgtgat cgacggagat
      121 ggtacaggca agccttatga gggaaaacag accatggatc ttgaagtcaa agagggcgga
      181 cctctgcctt ttgcctttga tatcctgacc actgcattcc attacggcaa cagggtattc
      241 gtgaaatatc cagacaacat acaagactat tttaagcagt cgtttcctaa ggggtattcg
      301 tgggaacgaa gcttgacttt cgaagacggg ggcatttgca atgccagaaa cgacataaca
      361 atggaagggg acactttcta taataaagtt cgattttatg gtaccaactt tcccgccaat
      421 ggtccagtta tgcagaagaa gacgctgaaa tgggagccct ccactgagaa aatgtatgtg
      481 cgtgatggag tgctgacggg tgatattgag atggctttgt tgcttgaagg aaatgcccat
      541 taccgatgtg acttcagaac tacttacaaa gctaaggaga agggtgtcaa gttaccaggc
      601 gcccactttg tggaccacgc cattgagatt ttaagccatg acaaagatta caacaaggtt
      661 aagctgtatg agcatgctgt tgctcattct ggattgcctg acaatgccag acgatccgga
      721 ctcagatctg gcagcggtgg aggcagcgca tccggcggaa gcggaagctc caccaggtcc
      781 gtgtcctcgt cctcctaccg caggatgttc ggcggcccgg gcaccgcgag ccggccgagc
      841 tccagccgga gctacgtgac tacgtccacc cgcacctaca gcctgggcag cgcgctgcgc
      901 cccagcacca gccgcagcct ctacgcctcg tccccgggcg gcgtgtatgc cacgcgctcc
      961 tctgccgtgc gcctgcggag cagcgtgccc ggggtgcggc tcctgcagga ctcggtggac
     1021 ttctcgctgg ccgacgccat caacaccgag ttcaagaaca cccgcaccaa cgagaaggtg
     1081 gagctgcagg agctgaatga ccgcttcgcc aactacatcg acaaggtgcg cttcctggag
     1141 cagcagaata agatcctgct ggccgagctc gagcagctca agggccaagg caagtcgcgc
     1201 ctgggggacc tctacgagga ggagatgcgg gagctgcgcc ggcaggtgga ccagctaacc
     1261 aacgacaaag cccgcgtcga ggtggagcgc gacaacctgg ccgaggacat catgcgcctc
     1321 cgggagaaat tgcaggagga gatgcttcag agagaggaag ccgaaaacac cctgcaatct
     1381 ttcagacagg atgttgacaa tgcgtctctg gcacgtcttg accttgaacg caaagtggaa
     1441 tctttgcaag aagagattgc ctttttgaag aaactccacg aagaggaaat ccaggagctg
     1501 caggctcaga ttcaggaaca gcatgtccaa atcgatgtgg atgtttccaa gcctgacctc
     1561 acggctgccc tgcgtgacgt acgtcagcaa tatgaaagtg tggctgccaa gaacctgcag
     1621 gaggcagaag aatggtacaa atccaagttt gctgacctct ctgaggctgc caaccggaac
     1681 aatgacgccc tgcgccaggc aaagcaggag tccactgagt accggagaca ggtgcagtcc
     1741 ctcacctgtg aagtggatgc ccttaaagga accaatgagt ccctggaacg ccagatgcgt
     1801 gaaatggaag agaactttgc cgttgaagct gctaactacc aagacactat tggccgcctg
     1861 caggatgaga ttcagaatat gaaggaggaa atggctcgtc accttcgtga ataccaagac
     1921 ctgctcaatg ttaagatggc ccttgacatt gagattgcca cctacaggaa gctgctggaa
     1981 ggcgaggaga gcaggatttc tctgcctctt ccaaactttt cctccctgaa cctgagggaa
     2041 actaatctgg attcactccc tctggttgat acccactcaa aaaggacact tctgattaag
     2101 acggttgaaa ctagagatgg acaggttatc aacgaaactt ctcagcatca cgatgacctt
     2161 gaataaggat ccaccggatc tagataactg atcataatca gccataccac attgtagaag
     2221 tatccgc
//
