LOCUS       sequence-88674-e        2951 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane Scaffold, Excitation: 503 / 569, Emission: 
            514 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2951)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 2951)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mGeos-M-PSD95-N-14
FEATURES             Location/Qualifiers
     source          1..2951
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      23..32
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2243..2923
                     /codon_start=1
                     /product="monomeric variant of green-to-red photoswitchable
                     fluorescent protein EosFP (McKinney et al., 2009)"
                     /label=mEos2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFMYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
ORIGIN
        1 gctaccggac tcagatctcg aggccaccat ggactgtctc tgtatagtga caaccaagaa
       61 ataccgctac caagatgaag acacgccccc tctggaacac agcccggccc acctccccaa
      121 ccaggccaat tctccccctg tgattgtcaa cacggacacc ctagaagccc caggatatga
      181 gttgcaggtg aatggaacag agggggagat ggagtatgag gagatcacat tggaaagggg
      241 taactcaggt ctgggcttca gcatcgcagg tggcactgac aacccgcaca tcggtgacga
      301 cccgtccatt tttatcacca agatcattcc tggtggggct gcagcccagg atggccgcct
      361 cagggtcaat gacagcatcc tgtttgtaaa tgaagtggat gttcgggagg tgacccattc
      421 agctgcggtg gaggccctca aagaggcagg ttccatcgtt cgcctctatg tcatgcgccg
      481 gaaaccccca gccgaaaagg tcatggagat caaactcatc aaagggccta aaggacttgg
      541 cttcagcatt gcggggggcg ttgggaacca gcacatccct ggagataaca gcatctatgt
      601 aacgaagatc atcgaaggag gtgctgccca caaggatggc aggttgcaga ttggagacaa
      661 gatcctggcg gtcaacagtg tggggctgga ggacgtcatg cacgaggatg ccgtggcagc
      721 cctgaagaac acatatgacg ttgtgtacct aaaggtggcc aagcccagca atgcctacct
      781 gagtgacagc tatgctcccc cagacatcac aacctcgtat tctcagcacc tggacaatga
      841 gatcagtcat agcagctact tgggcactga ctaccccaca gccatgaccc ccacttcccc
      901 tcggcgctac tcccctgtgg ccaaggacct gctgggggag gaagacattc cccgggaacc
      961 aaggcggatc gtgatccatc ggggctccac cggcctgggc ttcaacatcg tgggcggcga
     1021 ggatggtgaa ggcatcttca tctccttcat ccttgctggg ggtccagccg acctcagtgg
     1081 ggagctacgg aagggggacc agatcctgtc ggtcaatggt gttgacctcc gcaatgccag
     1141 tcacgaacag gctgccattg ccctgaagaa tgcgggtcag acggtcacga tcatcgctca
     1201 gtataaacca gaagagtata gtcgattcga ggccaagatc catgatcttc gggaacagct
     1261 catgaatagt agcctaggct cagggactgc atccttgcga agcaacccca agaggggctt
     1321 ctacattagg gccctgtttg attacgacaa gaccaaggac tgcggtttct tgagccaggc
     1381 cctgagcttc cgcttcgggg atgtgcttca tgtcattgac gctggtgacg aagagtggtg
     1441 gcaagcacgg cgggtccact ccgacagtga gaccgacgac attggcttca ttcccagcaa
     1501 acggcgggtc gagcgacgag agtggtcaag gttaaaggcc aaggactggg gctccagctc
     1561 tggatcacag ggtcgagaag actcggttct gagctatgag acggtgaccc agatggaagt
     1621 gcactatgct cgtcccatca tcatccttgg acccaccaaa gaccgtgcca acgatgatct
     1681 tctctccgag ttccccgaca agtttggatc ctgtgtccct catacgacac gtcctaagcg
     1741 ggaatatgag atagacggcc gggattacca ctttgtctcc tcccgggaga aaatggagaa
     1801 ggacatccag gcacacaagt tcattgaggc tggccagtac aacagccacc tctatgggac
     1861 cagcgtccag tctgtgcgag aggtagcaga gcaggggaag cactgcatcc tcgatgtctc
     1921 ggccaatgcc gtgcggcggc tgcaggcggc ccacctgcac cccatcgcca tcttcatccg
     1981 tccccgctcc ctggagaatg tgctagagat caataagcgg atcacagagg agcaagcccg
     2041 gaaagccttc gacagagcca cgaagctgga gcaggagttc acagagtgct tctcagccat
     2101 cgtagagggc gacagctttg aagagatcta tcacaaagtg aaacgtgtca ttgaagacct
     2161 ctcaggcccc tacatctggg tcccagcccg agagagactc ggcagcgcat ccggcggaag
     2221 cggaagcgga ccggtcgcca ccatgagtgc gattaagcca gacatgaaga tcaaactccg
     2281 tatggaaggc aacgtaaacg ggcaccactt tgtgatcgac ggagatggta caggcaagcc
     2341 ttttgaggga aaacagagta tggatcttga agtcaaagag ggcggacctc tgccttttgc
     2401 ctttgatatc ctgaccactg cattcatgta cggcaacagg gtattcgcca aatatccaga
     2461 caacatacaa gactatttta agcagtcgtt tcctaagggg tattcgtggg aacgaagctt
     2521 gactttcgaa gacgggggca tttgcattgc cagaaacgac ataacaatgg aaggggacac
     2581 tttctataat aaagttcgat tttatggtac caactttccc gccaatggtc cagttatgca
     2641 gaagaagacg ctgaaatggg agccctccac tgagaaaatg tatgtgcgtg atggagtgct
     2701 gacgggtgat attcatatgg ctttgttgct tgaaggaaat gcccattacc gatgtgactt
     2761 cagaactact tacaaagcta aggagaaggg tgtcaagtta ccaggctacc actttgtgga
     2821 ccactgcatt gagattttaa gccatgacaa agattacaac aaggttaagc tgtatgagca
     2881 tgctgttgct cattctggat tgcctgacaa tgccagacga taagcggccg cgactctaga
     2941 tcataatcag c
//
