LOCUS       sequence-88676-e        1928 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 503 / 569, Emission: 514 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1928)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 1928)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mGeos2-VESS-Actin-C-18
FEATURES             Location/Qualifiers
     source          1..1928
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             32..709
                     /codon_start=1
                     /product="truly monomeric reversibly photoswitchable 
                     fluorescent protein, suitable for nonlinear structured 
                     illumination microscopy (Zhang et al., 2016)"
                     /label=Skylan-NS
                     /note="mEos3.1 derivative that eliminates the green-to-red 
                     conversion"
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFSYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDVEMALLL
                     EGNAHYRCDSRTTYKAKEKGVKLPGAHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     CDS             32..709
                     /codon_start=1
                     /product="improved monomeric variant of green-to-red 
                     photoswitchable fluorescent protein EosFP (Zhang et al., 
                     2012)"
                     /label=mEos3.1
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFSYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDVEMALLL
                     EGNAHYRCDSRTTYKAKEKGVKLPGAHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
ORIGIN
        1 gtcagatccg ctagcgctac cggtcgccac catgagtgcg attaagccag acatgaagat
       61 caaactccgt atggaaggca acgtaaacgg gcaccacttt gtgatcgacg gagatggtac
      121 aggcaagcct tttgagggaa aacagagtat ggatcttgaa gtcaaagagg gcggacctct
      181 gccttttgcc tttgatatcc tgaccactgc attctcctac ggcaacaggg tattcgccaa
      241 atatccagac aacatacaag actattttaa gcagtcgttt cctaaggggt attcgtggga
      301 acgaagcttg actttcgaag acgggggcat ttgcaacgcc agaaacgaca taacaatgga
      361 aggggacact ttctataata aagttcgatt ttatggtacc aactttcccg ccaatggtcc
      421 agttatgcag aagaagacgc tgaaatggga gccctccact gagaaaatgt atgtgcgtga
      481 tggagtgctg acgggtgatg tagagatggc tttgttgctt gaaggaaatg cccattaccg
      541 atgtgactcc agaactactt acaaagctaa ggagaagggt gtcaagttac caggcgccca
      601 ctttgtggac cactgcattg agattttaag ccatgacaaa gattacaaca aggttaagct
      661 gtatgagcat gctgttgctc attctggatt gcctgacaat gccagacgat ccggactcag
      721 atctggcagc ggtggaggca gcgcatccgg cggaagcgga agcgatgatg atatcgccgc
      781 gctcgtcgtc gacaacggct ccggcatgtg caaggccggc ttcgcgggcg acgatgcccc
      841 ccgggccgtc ttcccctcca tcgtggggcg ccccaggcac cagggcgtga tggtgggcat
      901 gggtcagaag gattcctatg tgggcgacga ggcccagagc aagagaggca tcctcaccct
      961 gaagtacccc atcgagcacg gcatcgtcac caactgggac gacatggaga aaatctggca
     1021 ccacaccttc tacaatgagc tgcgtgtggc tcccgaggag caccccgtgc tgctgaccga
     1081 ggcccccctg aaccccaagg ccaaccgcga gaagatgacc cagatcatgt ttgagacctt
     1141 caacacccca gccatgtacg ttgctatcca ggctgtgcta tccctgtacg cctctggccg
     1201 taccactggc atcgtgatgg actccggtga cggggtcacc cacactgtgc ccatctacga
     1261 ggggtatgcc ctcccccatg ccatcctgcg tctggacctg gctggccggg acctgactga
     1321 ctacctcatg aagatcctca ccgagcgcgg ctacagcttc accaccacgg ccgagcggga
     1381 aatcgtgcgt gacattaagg agaagctgtg ctacgtcgcc ctggacttcg agcaagagat
     1441 ggccacggct gcttccagct cctccctgga gaagagctac gagctgcctg acggccaggt
     1501 catcaccatt ggcaatgagc ggttccgctg ccctgaggca ctcttccagc cttccttcct
     1561 gggcatggag tcctgtggca tccacgaaac taccttcaac tccatcatga agtgtgacgt
     1621 ggacatccgc aaagacctgt acgccaacac agtgctgtct ggcggcacca ccatgtaccc
     1681 tggcattgcc gacaggatgc agaaggagat cactgccctg gcacccagca caatgaagat
     1741 caagatcatt gctcctcctg agcgcaagta ctccgtgtgg atcggcggct ccatcctggc
     1801 ctcgctgtcc accttccagc agatgtggat cagcaagcag gagtatgacg agtccggccc
     1861 ctccatcgtc caccgcaaat gcttctaggg atccaccgga tctagataac tgatcataat
     1921 cagccata
//
