LOCUS       sequence-88677-e        6666 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytoskeleton, Excitation: 503 / 569, Emission: 514 / 
            581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6666)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 6666)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mGeos2-VESS-MyosinIIA-N-14
FEATURES             Location/Qualifiers
     source          1..6666
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      30..39
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5958..6638
                     /codon_start=1
                     /product="truly monomeric reversibly photoswitchable 
                     fluorescent protein, suitable for nonlinear structured 
                     illumination microscopy (Zhang et al., 2016)"
                     /label=Skylan-NS
                     /note="mEos3.1 derivative that eliminates the green-to-red 
                     conversion"
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFSYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDVEMALLL
                     EGNAHYRCDSRTTYKAKEKGVKLPGAHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     CDS             5958..6638
                     /codon_start=1
                     /product="improved monomeric variant of green-to-red 
                     photoswitchable fluorescent protein EosFP (Zhang et al., 
                     2012)"
                     /label=mEos3.1
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFSYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDVEMALLL
                     EGNAHYRCDSRTTYKAKEKGVKLPGAHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
ORIGIN
        1 cgctagcgct accggactca gatctcgagg ccaccatggc tcagcaggct gcagacaagt
       61 acctctatgt ggataaaaac ttcatcaata acccgctggc ccaagctgac tgggctgcca
      121 agaagttggt atgggtgcct tccagcaaga atggctttga accagccagc ctcaaggagg
      181 aggtgggaga agaggccatt gtagagctgg tagagaatgg gaagaaggtg aaggtgaaca
      241 aggacgacat ccagaagatg aacccaccca agttctccaa ggtggaggac atggcagagc
      301 tcacgtgcct caacgaagct tcggtgctgc acaacctcaa ggagcgatac tactcagggc
      361 ttatctacac ctattcaggc ctgttctgtg tggtcatcaa cccttataag aacctgccca
      421 tctactcaga ggagatcgtg gagatgtaca agggcaagaa gaggcacgag atgccacccc
      481 acatctacgc catcacagat actgcctacc ggagcatgat gcaggaccgg gaagatcagt
      541 ccatcctgtg cacgggggag tctggagcag ggaagacaga gaacaccaag aaagtcatcc
      601 agtacctggc acatgtggcc tcctcacaca agagcaagaa ggaccagggg gagttggagc
      661 ggcagctgct acaggccaac cctatcctag aggcctttgg aaacgccaag acggtgaaga
      721 atgacaactc ctctcgattc ggtaaattca ttcgtatcaa ctttgatgtc aatggctaca
      781 ttgttggtgc caacattgag acttatcttc tggagaaatc tcgtgctatc cgccaagcca
      841 aagaggagcg gaccttccac atcttctact acctgctgtc tggggccgga gaacacctga
      901 agactgatct cctgttggag ccatacaaca aataccgctt cctgtccaac gggcacgtca
      961 ccatccctgg gcagcaggac aaggacatgt tccaggagac aatggaggcc atgagaatta
     1021 tgggtatccc agaggatgag cagatgggct tgctgcgggt catctctggg gtccttcagc
     1081 ttggcaacat tgccttcaag aaggagcgga acactgacca ggcgtccatg ccggacaaca
     1141 cagctgctca aaaggtgtcc cacctcctgg ggatcaatgt gaccgacttc accagaggca
     1201 tcctcacccc acgcatcaag gtgggcagag actatgtgca gaaggcgcag actaaagagc
     1261 aggctgactt tgccattgag gccttggcca aggctaccta tgagcggatg ttccgctggc
     1321 tggtgcttcg catcaacaaa gctctggaca agaccaagag gcagggcgcc tcatttatcg
     1381 ggatactgga catcgctggc tttgaaatct ttgatctgaa ctccttcgag cagctgtgca
     1441 tcaactacac caacgagaag ctgcagcagc tgttcaacca caccatgttc atcctggagc
     1501 aggaggagta ccagcgagag ggcatcgagt ggaacttcat cgacttcggc ctggacctgc
     1561 agccctgcat cgacctcatt gagaagccgg cgggtccccc aggcatcctg gccctgctag
     1621 atgaggagtg ctggtttcct aaggccactg acaagagctt cgtggagaag gtggtgcagg
     1681 agcagggcac ccaccccaag ttccagaagc ccaagcaact gaaggacaag gctgatttct
     1741 gcattatcca ctatgccggc aaggtggact ataaagctga cgagtggctg atgaagaaca
     1801 tggacccctt gaacgacaac atcgccacgc tgcttcacca gtcctcagac aagtttgtct
     1861 ctgagctgtg gaaggatgtg gatcggatca ttggcttgga ccaagtggct ggaatgtccg
     1921 agacagcact acctggtgcc ttcaagaccc ggaagggcat gttccgtact gtcggacagc
     1981 tgtacaagga gcagctggcc aagctcatgg ccacgttgag gaataccaac cccaacttcg
     2041 tgcgctgcat cattcccaac catgagaaga aggccggcaa actggacccg cacttggtgc
     2101 tggaccagct gcgctgcaat ggcgtccttg agggcatccg gatctgccgc cagggctttc
     2161 ccaacagggt ggtcttccag gagttccggc agaggtatga gatcctcacc cccaactcca
     2221 tcccgaaggg cttcatggat ggcaagcaag cgtgtgtgct catgatcaaa gccttggagc
     2281 ttgacagcaa cctgtaccgc atcggccaga gcaaagtgtt cttccgggca ggagtgctcg
     2341 cccacctgga ggaagagcgg gacctgaaga tcaccgatgt catcattggc ttccaggcct
     2401 gctgcagggg ctacctggcc aggaaggcct ttgccaagag gcagcaacag ctgaccgcca
     2461 tgaaggtcct acagaggaac tgtgctgcgt acctcaggct gcgcaactgg cagtggtgga
     2521 ggctcttcac caaggtcaag cccctgttga actcaataag acatgaggat gagctgttag
     2581 ccaaggaggc ggaactgaca aaggttcgag agaaacatct ggctgcagag aacaggctga
     2641 cagagatgga gacgatgcag tctcagctca tggcagagaa gctgcagctt caggagcagc
     2701 tgcaggcgga gacagagctg tgtgccgagg ctgaggagct ccgggcccgt ctgacagcga
     2761 agaagcagga gctggaggag atatgccatg acctggaggc cagggtggag gaggaggagg
     2821 agcgctgcca gtacctgcag gccgagaaga agaagatgca gcagaacatc caggaacttg
     2881 aggagcagtt ggaggaggag gagagcgccc ggcagaagct gcagcttgag aaggtgacca
     2941 ccgaggccaa gctgaagaaa ctggaggagg accagatcat catggaggac cagaactgca
     3001 aactggccaa ggagaagaaa ctgctggaag acagagtagc tgagttcact accaacctca
     3061 tggaagagga ggagaagtcc aagagcctgg ccaagctcaa gaacaagcac gaggcaatga
     3121 tcaccgacct ggaagagcgc ctccgtaggg aggagaagca gaggcaggag ttggagaaga
     3181 cccgtcgcaa gctggaggga gactccacag acctcagtga ccagattgct gagctccagg
     3241 cgcagatagc agagctcaag atgcagctgg ccaagaagga ggaggagttg caggctgcct
     3301 tggccagagt ggaagaagaa gctgctcaga agaatatggc cctgaagaag atccgagaac
     3361 tggaaactca gatatctgag ctccaggagg acctggagtc ggagcgagcc tccaggaata
     3421 aagccgagaa gcagaaacgg gatctgggag aggagctgga ggcgctgaag acagagctgg
     3481 aggacacgct ggactccacg gctgcccagc aggagctgag gtcgaagcgt gagcaggagg
     3541 tgagcatcct gaagaagact ctggaggacg aggccaagac ccatgaggcc cagatccagg
     3601 agatgaggca gaagcactca caggctgtgg aggagctggc agatcagttg gagcagacga
     3661 agcgggtaaa agctaccctt gagaaggcga agcagaccct ggagaatgag cggggagagc
     3721 tggccaatga ggtgaaggcc ctgctgcaag gcaagggcga ctcagagcac aagcgcaaga
     3781 aggtggaggc gcagctgcaa gaactgcagg tcaagttcag cgagggagag cgcgtgcgaa
     3841 ccgaactggc cgacaaggtc accaagctgc aggttgaact ggacagtgtg accggtctcc
     3901 ttagccagtc tgacagcaag tccagcaagc ttacgaagga cttctctgcg ctggagtccc
     3961 agcttcagga cacacaggag ttgctccagg aggagaaccg gcagaagctg agcctgagca
     4021 ccaagctcaa gcagatggag gatgagaaaa actccttcag ggagcagctg gaggaggagg
     4081 aggaggccaa gcgcaacttg gagaagcaga tcgccacgct ccatgcccag gtgaccgaca
     4141 tgaagaagaa gatggaggac ggtgtagggt gcctggagac tgcagaggag gcgaagcgga
     4201 ggcttcagaa ggacttggaa ggcctgagcc agcggcttga ggagaaggtg gctgcctacg
     4261 ataagctgga gaagaccaag acacggctgc agcaggagct ggacgacctg ctggttgacc
     4321 tggaccacca gcggcagagc gtctccaacc tggaaaagaa gcagaagaag ttcgaccagc
     4381 tcctagccga ggagaagacc atctcggcca agtatgcaga ggagcgtgac cgagctgagg
     4441 ctgaggcccg tgagaaggag acaaaggcgc tatcactggc ccgggcgctt gaggaggcca
     4501 tggagcagaa ggcagagctg gagcggctca acaagcagtt ccgcacggag atggaggacc
     4561 tcatgagctc caaggatgac gtgggcaaga gtgtccacga gctggagaag tccaagcggg
     4621 ccttggagca gcaggtggag gagatgaaga cccagctgga ggagctggag gatgagctgc
     4681 aggccacgga ggatgccaag ctccgcctgg aggtgaacct gcaggccatg aaggcccagt
     4741 ttgagcggga tctgcagggc cgggatgaac agagcgagga gaagaagaag cagctggtca
     4801 gacaggtgcg ggagatggag gcggagctgg aggatgagag gaagcagcgc tccatggcca
     4861 tggccgcacg caagaaactg gagatggatc tgaaggacct ggaggcacac attgacacag
     4921 ccaataagaa ccgggaagag gccatcaaac agctgcggaa gcttcaggcc cagatgaagg
     4981 actgcatgcg ggagctggac gacacgcgcg cctcccggga ggagatcctg gcgcaggcca
     5041 aggagaatga gaagaagctg aagagcatgg aggccgagat gattcagctg caggaggaac
     5101 tggcagctgc tgagcgtgct aagcgtcagg cccaacagga acgggacgag ctggctgatg
     5161 agatcgccaa cagcagtggc aaaggggccc tagcattaga ggagaagcgg cgactggagg
     5221 cccgcattgc cctgctggag gaggagctgg aggaggaaca gggcaacacg gagctgatca
     5281 acgatcggct gaagaaggcc aacctgcaga tcgaccaaat aaacaccgac ctgaacctgg
     5341 aacgcagcca cgcacagaag aatgagaatg cgcgacagca gctggaacgc cagaacaagg
     5401 agctcaaggc caagctgcag gaaatggaga gtgctgtcaa gtccaaatac aaggcctcca
     5461 tcgcggcctt ggaggccaaa attgcacagc tggaggaaca gctggacaac gagaccaagg
     5521 agcgccaggc agcctccaag caggtgcgcc ggacggagaa gaagctgaag gacgtgctgc
     5581 tgcaggtgga ggacgagcgg aggaacgcgg aacagttcaa ggaccaggct gacaaggcgt
     5641 ccacccgcct gaagcagctt aaacggcagc tagaggaggc tgaagaggag gcccagcggg
     5701 ccaatgcctc acgccggaag ctgcagcgtg agctggaaga tgccacagag accgctgatg
     5761 ctatgaaccg cgaggtcagc tccctgaaga acaaactgag gcgtggggac ctgccatttg
     5821 tcgtgactcg ccgaattgtt cggaaaggca ctggcgactg ctcagacgag gaggtcgacg
     5881 gtaaagcaga tggggccgat gccaaggcag ctgaaggcgg aagcggcggg agcgcagggg
     5941 atccaccggt cgccaccatg agtgcgatta agccagacat gaagatcaaa ctccgtatgg
     6001 aaggcaacgt aaacgggcac cactttgtga tcgacggaga tggtacaggc aagccttttg
     6061 agggaaaaca gagtatggat cttgaagtca aagagggcgg acctctgcct tttgcctttg
     6121 atatcctgac cactgcattc tcctacggca acagggtatt cgccaaatat ccagacaaca
     6181 tacaagacta ttttaagcag tcgtttccta aggggtattc gtgggaacga agcttgactt
     6241 tcgaagacgg gggcatttgc aacgccagaa acgacataac aatggaaggg gacactttct
     6301 ataataaagt tcgattttat ggtaccaact ttcccgccaa tggtccagtt atgcagaaga
     6361 agacgctgaa atgggagccc tccactgaga aaatgtatgt gcgtgatgga gtgctgacgg
     6421 gtgatgtaga gatggctttg ttgcttgaag gaaatgccca ttaccgatgt gactccagaa
     6481 ctacttacaa agctaaggag aagggtgtca agttaccagg cgcccacttt gtggaccact
     6541 gcattgagat tttaagccat gacaaagatt acaacaaggt taagctgtat gagcatgctg
     6601 ttgctcattc tggattgcct gacaatgcca gacgataagc ggccgcgact ctagatcata
     6661 atcagc
//
