LOCUS       sequence-88678-e        2494 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 503 / 569, Emission: 514 
            / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2494)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 2494)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mGeos2-VESS-Paxillin-22
FEATURES             Location/Qualifiers
     source          1..2494
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    1703..1755
                     /label=MCS
                     /note="multiple cloning site"
     CDS             1769..2449
                     /codon_start=1
                     /product="truly monomeric reversibly photoswitchable 
                     fluorescent protein, suitable for nonlinear structured 
                     illumination microscopy (Zhang et al., 2016)"
                     /label=Skylan-NS
                     /note="mEos3.1 derivative that eliminates the green-to-red 
                     conversion"
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFSYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDVEMALLL
                     EGNAHYRCDSRTTYKAKEKGVKLPGAHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     CDS             1769..2449
                     /codon_start=1
                     /product="improved monomeric variant of green-to-red 
                     photoswitchable fluorescent protein EosFP (Zhang et al., 
                     2012)"
                     /label=mEos3.1
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFSYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDVEMALLL
                     EGNAHYRCDSRTTYKAKEKGVKLPGAHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
ORIGIN
        1 gttgaacgtc agatccgcta gcaccatgga cgacctcgat gccttactgg cagacctgga
       61 atccaccacc tcgcatatct ctaagcgacc ggtgtttcta acagaggaaa cgccttactc
      121 ctacccaact ggaaaccata cgtaccagga gattgctgtg ccaccgccgg tgcccccgcc
      181 gccttccagt gaggccctaa atggcactgt gattgacccc ttagaccagt ggcaacccag
      241 cgtgtccaga tacggccacc agcaacctca gtcccagtct ccgatataca gctctagtgc
      301 caaaagctcc agtgcctctg ttcctagaga cgggctcagc tccccttctc cccgtgccag
      361 tgaggaggaa cacgtgtaca gcttcccaaa caagcagaag tctgcagaac catctcccac
      421 aatgaccagc acctccctgg gcagcaacct ctcggaactg gacaggcttc tcctggaact
      481 gaatgctgtt caacataatc cccccagtgg cttctcagca gatgaagtca gcagaagccc
      541 atcactgccc aatgtgaccg gacctcacta tgtcatccca gagagcagca gctctgcagg
      601 agggaaggct gcacccccta caaaagaaaa accaaagcgg aatggtgggc gtgggattga
      661 agatgtgcgt cccagtgtgg agagcctgct ggatgagctg gagagctctg tgccaagccc
      721 agtccctgca atcactgtga gccaagggga ggtgagcagc ccccagcgtg tcaacgccag
      781 tcagcagcag acccgtatct ctgcttcttc agctacacga gaactggatg agctgatggc
      841 gtccctctct gactttaagt tcatggcaca ggggaaagcc ggcggcagca gctccccacc
      901 atccaccacc ccaaagcctg gcagtcagct ggacaccatg ctgggaagtc tgcagtctga
      961 cctgaacaaa ctgggggtag cgacggtcgc caagggtgtg tgcggagcct gcaagaagcc
     1021 gattgctggg caggtagtta cagccatggg gaagacctgg caccctgagc acttcgtctg
     1081 cacccactgc caggaggaga ttggatcacg gaacttcttt gagcgggatg ggcagcccta
     1141 ctgtgagaag gactatcaca acctcttctc tcctcgctgc tactactgca atgggccaat
     1201 ccttgataaa gtggtgacgg ctttggacag gacgtggcac cctgaacact ttttctgtgc
     1261 ccagtgtgga gttttctttg gacctgaagg atttcatgag aaggatggca aagcctattg
     1321 ccgcaaggac tactttgaca tgtttgctcc caagtgtgga ggctgtgccc gggctatcct
     1381 ggaaaattac atttctgcct taaacaccct gtggcaccct gagtgctttg tctgtcggga
     1441 atgtttcaca ccgttcatca acggcagctt cttcgagcac gacggccagc cctactgcga
     1501 ggtgcattac cacgagcgcc gcggctcgct gtgctctggc tgccagaagc ccatcacggg
     1561 acgctgcatc actgccatgg gcaagaaatt tcaccccgaa cactttgtct gtgccttctg
     1621 cctcaagcag ctcaacaaag gaaccttcaa agaacagaac gacaagccct actgtcagaa
     1681 ctgctttctc aaactcttct gtctcgagct caagcttcga attctgcagt cgacggtacc
     1741 gcgggcccgg gatccaccgg tcgccaccat gagtgcgatt aagccagaca tgaagatcaa
     1801 actccgtatg gaaggcaacg taaacgggca ccactttgtg atcgacggag atggtacagg
     1861 caagcctttt gagggaaaac agagtatgga tcttgaagtc aaagagggcg gacctctgcc
     1921 ttttgccttt gatatcctga ccactgcatt ctcctacggc aacagggtat tcgccaaata
     1981 tccagacaac atacaagact attttaagca gtcgtttcct aaggggtatt cgtgggaacg
     2041 aagcttgact ttcgaagacg ggggcatttg caacgccaga aacgacataa caatggaagg
     2101 ggacactttc tataataaag ttcgatttta tggtaccaac tttcccgcca atggtccagt
     2161 tatgcagaag aagacgctga aatgggagcc ctccactgag aaaatgtatg tgcgtgatgg
     2221 agtgctgacg ggtgatgtag agatggcttt gttgcttgaa ggaaatgccc attaccgatg
     2281 tgactccaga actacttaca aagctaagga gaagggtgtc aagttaccag gcgcccactt
     2341 tgtggaccac tgcattgaga ttttaagcca tgacaaagat tacaacaagg ttaagctgta
     2401 tgagcatgct gttgctcatt ctggattgcc tgacaatgcc agacgataag cggccgcgac
     2461 tctagatcat aatcagccat accacattgt agaa
//
