LOCUS       sequence-88679-e        1054 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 503 / 569, Emission: 514 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1054)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 1054)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mGeos2-VEMS-Actin-C-18
FEATURES             Location/Qualifiers
     source          1..1054
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             48..725
                     /codon_start=1
                     /product="truly monomeric reversibly photoswitchable 
                     fluorescent protein, suitable for nonlinear structured 
                     illumination microscopy (Zhang et al., 2016)"
                     /label=Skylan-NS
                     /note="mEos3.1 derivative that eliminates the green-to-red 
                     conversion"
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFMYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDVEMALLL
                     EGNAHYRCDSRTTYKAKEKGVKLPGAHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
ORIGIN
        1 acattgtcta gtgaccgtca gatccgctag cgctaccggt cgccaccatg agtgcgatta
       61 agccagacat gaagatcaaa ctccgtatgg aaggcaacgt aaacgggcac cactttgtga
      121 tcgacggaga tggtacaggc aagccttttg agggaaaaca gagtatggat cttgaagtca
      181 aagagggcgg acctctgcct tttgcctttg atatcctgac cactgcattc atgtacggca
      241 acagggtatt cgccaaatat ccagacaaca tacaagacta ttttaagcag tcgtttccta
      301 aggggtattc gtgggaacga agcttgactt tcgaagacgg gggcatttgc aacgccagaa
      361 acgacataac aatggaaggg gacactttct ataataaagt tcgattttat ggtaccaact
      421 ttcccgccaa tggtccagtt atgcagaaga agacgctgaa atgggagccc tccactgaga
      481 aaatgtatgt gcgtgatgga gtgctgacgg gtgatgtaga gatggctttg ttgcttgaag
      541 gaaatgccca ttaccgatgt gactccagaa ctacttacaa agctaaggag aagggtgtca
      601 agttaccagg cgcccacttt gtggaccact gcattgagat tttaagccat gacaaagatt
      661 acaacaaggt taagctgtat gagcatgctg ttgctcattc tggattgcct gacaatgcca
      721 gacgatccgg actcagatct ggcagcggtg gaggcagcgc atccggcgga agcggaagcg
      781 atgatgatat cgccgcgctc gtcgtcgaca acggctccgg catgtgcaag gccggcttcg
      841 cgggcgacga tgccccccgg gccgtcttcc cctccatcgt ggggcgcccc aggcaccagg
      901 gcgtgatggt gggcatgggt cagaaggatt cctatgtggg cgacgaggcc cagagcaaga
      961 gaggcatcct caccctgaag taccccatcg agcacggcat cgtcaccaac tgggacgaca
     1021 tggagaaatc tggcaccaca ccttctacat gagc
//
