LOCUS       sequence-88681-e        1148 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 503 / 569, Emission: 514 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1148)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 1148)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mGeos2-VES-Actin-C-18
FEATURES             Location/Qualifiers
     source          1..1148
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             49..726
                     /codon_start=1
                     /product="truly monomeric reversibly photoswitchable 
                     fluorescent protein, suitable for nonlinear structured 
                     illumination microscopy (Zhang et al., 2016)"
                     /label=Skylan-NS
                     /note="mEos3.1 derivative that eliminates the green-to-red 
                     conversion"
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFSYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDVEMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     CDS             49..726
                     /codon_start=1
                     /product="improved monomeric variant of green-to-red 
                     photoswitchable fluorescent protein EosFP (Zhang et al., 
                     2012)"
                     /label=mEos3.1
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFSYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDVEMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
ORIGIN
        1 aggggatggt aagtgacgtc agatccgcta gcgctaccgg tcgccaccat gagtgcgatt
       61 aagccagaca tgaagatcaa actccgtatg gaaggcaacg taaacgggca ccactttgtg
      121 atcgacggag atggtacagg caagcctttt gagggaaaac agagtatgga tcttgaagtc
      181 aaagagggcg gacctctgcc ttttgccttt gatatcctga ccactgcatt ctcctacggc
      241 aacagggtat tcgccaaata tccagacaac atacaagact attttaagca gtcgtttcct
      301 aaggggtatt cgtgggaacg aagcttgact ttcgaagacg ggggcatttg caacgccaga
      361 aacgacataa caatggaagg ggacactttc tataataaag ttcgatttta tggtaccaac
      421 tttcccgcca atggtccagt tatgcagaag aagacgctga aatgggagcc ctccactgag
      481 aaaatgtatg tgcgtgatgg agtgctgacg ggtgatgtag agatggcttt gttgcttgaa
      541 ggaaatgccc attaccgatg tgacttcaga actacttaca aagctaagga gaagggtgtc
      601 aagttaccag gcgcccactt tgtggaccac tgcattgaga ttttaagcca tgacaaagat
      661 tacaacaagg ttaagctgta tgagcatgct gttgctcatt ctggattgcc tgacaatgcc
      721 agacgatccg gactcagatc tggcagcggt ggaggcagcg catccggcgg aagcggaagc
      781 gatgatgata tcgccgcgct cgtcgtcgac aacggctccg gcatgtgcaa ggccggcttc
      841 gcgggcgacg atgccccccg ggccgtcttc ccctccatcg tggggcgccc caggcaccag
      901 ggcgtgatgg tgggcatggg tcagaaggat tcctatgtgg gcgacgaggc ccagagcaag
      961 agaggcatcc tcaccctgaa gtaccccatc gagcacggca tcgtcaccaa ctgggacgac
     1021 atggagaaaa tctggcacac accttctaca tgagctgcgt ggtggctccg aggagcaccc
     1081 cgtgctgctg acgaggcccc ctgaccccaa ggcaccgcga gagatgaccc agatcatgtt
     1141 gagactca
//
