LOCUS       sequence-88684-e        1933 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 503 / 569, Emission: 514 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1933)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 1933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mGeos2-VEL-Actin-C-18
FEATURES             Location/Qualifiers
     source          1..1933
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             39..716
                     /codon_start=1
                     /product="truly monomeric reversibly photoswitchable 
                     fluorescent protein, suitable for nonlinear structured 
                     illumination microscopy (Zhang et al., 2016)"
                     /label=Skylan-NS
                     /note="mEos3.1 derivative that eliminates the green-to-red 
                     conversion"
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFLYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDVEMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
ORIGIN
        1 tagtgacgtc agatccgcta gcgctaccgg tcgccaccat gagtgcgatt aagccagaca
       61 tgaagatcaa actccgtatg gaaggcaacg taaacgggca ccactttgtg atcgacggag
      121 atggtacagg caagcctttt gagggaaaac agagtatgga tcttgaagtc aaagagggcg
      181 gacctctgcc ttttgccttt gatatcctga ccactgcatt cctgtacggc aacagggtat
      241 tcgccaaata tccagacaac atacaagact attttaagca gtcgtttcct aaggggtatt
      301 cgtgggaacg aagcttgact ttcgaagacg ggggcatttg caacgccaga aacgacataa
      361 caatggaagg ggacactttc tataataaag ttcgatttta tggtaccaac tttcccgcca
      421 atggtccagt tatgcagaag aagacgctga aatgggagcc ctccactgag aaaatgtatg
      481 tgcgtgatgg agtgctgacg ggtgatgtag agatggcttt gttgcttgaa ggaaatgccc
      541 attaccgatg tgacttcaga actacttaca aagctaagga gaagggtgtc aagttaccag
      601 gcgcccactt tgtggaccac tgcattgaga ttttaagcca tgacaaagat tacaacaagg
      661 ttaagctgta tgagcatgct gttgctcatt ctggattgcc tgacaatgcc agacgatccg
      721 gactcagatc tggcagcggt ggaggcagcg catccggcgg aagcggaagc gatgatgata
      781 tcgccgcgct cgtcgtcgac aacggctccg gcatgtgcaa ggccggcttc gcgggcgacg
      841 atgccccccg ggccgtcttc ccctccatcg tggggcgccc caggcaccag ggcgtgatgg
      901 tgggcatggg tcagaaggat tcctatgtgg gcgacgaggc ccagagcaag agaggcatcc
      961 tcaccctgaa gtaccccatc gagcacggca tcgtcaccaa ctgggacgac atggagaaaa
     1021 tctggcacca caccttctac aatgagctgc gtgtggctcc cgaggagcac cccgtgctgc
     1081 tgaccgaggc ccccctgaac cccaaggcca accgcgagaa gatgacccag atcatgtttg
     1141 agaccttcaa caccccagcc atgtacgttg ctatccaggc tgtgctatcc ctgtacgcct
     1201 ctggccgtac cactggcatc gtgatggact ccggtgacgg ggtcacccac actgtgccca
     1261 tctacgaggg gtatgccctc ccccatgcca tcctgcgtct ggacctggct ggccgggacc
     1321 tgactgacta cctcatgaag atcctcaccg agcgcggcta cagcttcacc accacggccg
     1381 agcgggaaat cgtgcgtgac attaaggaga agctgtgcta cgtcgccctg gacttcgagc
     1441 aagagatggc cacggctgct tccagctcct ccctggagaa gagctacgag ctgcctgacg
     1501 gccaggtcat caccattggc aatgagcggt tccgctgccc tgaggcactc ttccagcctt
     1561 ccttcctggg catggagtcc tgtggcatcc acgaaactac cttcaactcc atcatgaagt
     1621 gtgacgtgga catccgcaaa gacctgtacg ccaacacagt gctgtctggc ggcaccacca
     1681 tgtaccctgg cattgccgac aggatgcaga aggagatcac tgccctggca cccagcacaa
     1741 tgaagatcaa gatcattgct cctcctgagc gcaagtactc cgtgtggatc ggcggctcca
     1801 tcctggcctc gctgtccacc ttccagcaga tgtggatcag caagcaggag tatgacgagt
     1861 ccggcccctc catcgtccac cgcaaatgct tctagggatc caccggatct agataactga
     1921 tcataatcag cca
//
