LOCUS       sequence-88685-e        2566 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 503 / 569, 
            Emission: 514 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2566)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 2566)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mGeos2-VEL-Calnexin-N-14
FEATURES             Location/Qualifiers
     source          1..2566
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1859..2539
                     /codon_start=1
                     /product="truly monomeric reversibly photoswitchable 
                     fluorescent protein, suitable for nonlinear structured 
                     illumination microscopy (Zhang et al., 2016)"
                     /label=Skylan-NS
                     /note="mEos3.1 derivative that eliminates the green-to-red 
                     conversion"
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFLYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDVEMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
ORIGIN
        1 agatccgcta gcgctaccgg actcagatct cgagaccgcc atggaaggga agtggttgct
       61 gtgtatgtta ctggtgcttg gaactgctat tgttgaggct catgatggac atgatgatga
      121 tgtgattgat attgaggatg accttgacga tgtcattgaa gaggtagaag actcaaaacc
      181 agataccact gctcctcctt catctcccaa ggttacttac aaagctccag ttccaacagg
      241 ggaagtatat tttgctgatt cttttgacag aggaactctg tcagggtgga ttttatccaa
      301 agccaagaaa gacgataccg atgatgaaat tgccaaatat gatggaaagt gggaggtaga
      361 ggaaatgaag gagtcaaagc ttccaggtga taaaggactt gtgttgatgt ctcgggccaa
      421 gcatcatgcc atctctgcta aactgaacaa gcccttcctg tttgacacca agcctctcat
      481 tgttcagtat gaggttaatt tccaaaatgg aatagaatgt ggtggtgcct atgtgaaact
      541 gctttctaaa acaccagaac tcaacctgga tcagttccat gacaagaccc cttatacgat
      601 tatgtttggt ccagataaat gtggagagga ctataaactg cacttcatct tccgacacaa
      661 aaaccccaaa acgggtatct atgaagaaaa acatgctaag aggccagatg cagatctgaa
      721 gacctatttt actgataaga aaacacatct ttacacacta atcttgaatc cagataatag
      781 ttttgaaata ctggttgacc aatctgtggt gaatagtgga aatctgctca atgacatgac
      841 tcctcctgta aatccttcac gtgaaattga ggacccagaa gaccggaagc ccgaggattg
      901 ggatgaaaga ccaaaaatcc cagatccaga agctgtcaag ccagatgact gggatgaaga
      961 tgcccctgct aagattccag atgaagaggc cacaaaaccc gaaggctggt tagatgatga
     1021 gcctgagtac gtacctgatc cagacgcaga gaaacctgag gattgggatg aagacatgga
     1081 tggagaatgg gaggctcctc agattgccaa ccctagatgt gagtcagctc ctggatgtgg
     1141 tgtctggcag cgacctgtga ttgacaaccc caattataaa ggcaaatgga agcctcctat
     1201 gattgacaat cccagttacc agggaatctg gaaacccagg aaaataccaa atccagattt
     1261 ctttgaagat ctggaacctt tcagaatgac tccttttagt gctattggtt tggagctgtg
     1321 gtccatgacc tctgacattt tttttgacaa ctttatcatt tgtgctgatc gaagaatagt
     1381 tgatgattgg gccaatgatg gatggggcct gaagaaagct gctgatgggg ctgctgagcc
     1441 aggcgttgtg gggcagatga acgaggcagc tgaagagcgc ccgtggctgt gggtagtcta
     1501 tattctaact gtagcccttc ctgtgttcct ggttatcctc ttctgctgtt ctggaaagaa
     1561 acagaccagt ggtatggagt ataagaaaac tgatgcacct caaccggatg tgaaggaaga
     1621 ggaagaagag aaggaagagg aaaaggacaa gggagatgag gaggaggaag gagaagagaa
     1681 acttgaagag aaacagaaaa gtgatgctga agaagatggt ggcactgtca gtcaagagga
     1741 ggaagacaga aaacctaaag cagaggagga tgaaattttg aacagatcac caagaaacag
     1801 aaagccacga agagaggggg gtgcgtcagg cggcagcgga tctggaccgg tcgccaccat
     1861 gagtgcgatt aagccagaca tgaagatcaa actccgtatg gaaggcaacg taaacgggca
     1921 ccactttgtg atcgacggag atggtacagg caagcctttt gagggaaaac agagtatgga
     1981 tcttgaagtc aaagagggcg gacctctgcc ttttgccttt gatatcctga ccactgcatt
     2041 cctgtacggc aacagggtat tcgccaaata tccagacaac atacaagact attttaagca
     2101 gtcgtttcct aaggggtatt cgtgggaacg aagcttgact ttcgaagacg ggggcatttg
     2161 caacgccaga aacgacataa caatggaagg ggacactttc tataataaag ttcgatttta
     2221 tggtaccaac tttcccgcca atggtccagt tatgcagaag aagacgctga aatgggagcc
     2281 ctccactgag aaaatgtatg tgcgtgatgg agtgctgacg ggtgatgtag agatggcttt
     2341 gttgcttgaa ggaaatgccc attaccgatg tgacttcaga actacttaca aagctaagga
     2401 gaagggtgtc aagttaccag gcgcccactt tgtggaccac tgcattgaga ttttaagcca
     2461 tgacaaagat tacaacaagg ttaagctgta tgagcatgct gttgctcatt ctggattgcc
     2521 tgacaatgcc agacgataag cggccgcgac tctagatcat aatcag
//
