LOCUS       sequence-88689-e        2387 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane/Vesicles, Excitation: 503 / 569, Emission: 
            514 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2387)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 2387)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mGeos2-VEL-C-Src-7
FEATURES             Location/Qualifiers
     source          1..2387
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             51..92
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     CDS             1671..2351
                     /codon_start=1
                     /product="truly monomeric reversibly photoswitchable 
                     fluorescent protein, suitable for nonlinear structured 
                     illumination microscopy (Zhang et al., 2016)"
                     /label=Skylan-NS
                     /note="mEos3.1 derivative that eliminates the green-to-red 
                     conversion"
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFLYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDVEMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
ORIGIN
        1 agatccgcta gcgctaccgg actcagatct cgagctcaag cttcgaattc atggggagca
       61 gcaagagcaa gcccaaggac cccagccagc gccggcgcag cctggagcca cccgacagca
      121 cccaccacgg gggattccca gcctcgcaga cccccaacaa gacagcagcc cccgacacgc
      181 accgcacccc cagccgctcc tttgggaccg tggccaccga gcccaagctc ttcgggggct
      241 tcaacacttc tgacaccgtc acgtcgccgc agcgtgccgg ggcactggct ggcggcgtca
      301 ccactttcgt ggctctctat gactacgagt cccggactga aacggacttg tccttcaaga
      361 aaggagaacg cctgcagatt gtcaacaaca cggaaggtga ctggtggctg gctcattccc
      421 tcactacagg acagacgggc tacatcccca gtaactatgt cgcgccctca gactccatcc
      481 aggctgaaga gtggtacttt gggaagatca ctcgtcggga gtccgagcgg ctgctgctca
      541 accccgaaaa cccccgggga accttcttgg tccgggagag cgagacgaca aaaggtgcct
      601 attgcctctc cgtttctgac tttgacaacg ccaaggggct caatgtgaag cactacaaga
      661 tccgcaagct ggacagcggc ggcttctaca tcacctcacg cacacagttc agcagcctgc
      721 agcagctggt ggcctactac tccaaacatg ctgatggctt gtgccaccgc ctgaccaacg
      781 tctgccccac gtccaagccc cagacccagg gactcgccaa ggacgcgtgg gaaatccccc
      841 gggagtcgct gcggctggag gtgaagctgg ggcagggctg ctttggagag gtctggatgg
      901 ggacctggaa cggcaccacc agagtggcca taaagactct gaagcccggc accatgtccc
      961 cggaggcctt cctgcaggaa gcccaagtga tgaagaagct ccggcatgag aagctggttc
     1021 agctgtacgc agtggtgtcg gaagagccca tctacatcgt cactgagtac atgagcaagg
     1081 ggagcctcct ggatttcctg aagggagaga tgggcaagta cctgcggctg ccacagctcg
     1141 tcgatatggc tgctcagatt gcatccggca tggcctatgt ggagaggatg aactacgtgc
     1201 accgagacct gcgggcggcc aacatcctgg tgggggagaa cctggtgtgc aaggtggctg
     1261 actttgggct ggcacgcctc atcgaggaca acgagtacac agcacggcaa ggtgccaagt
     1321 tccccatcaa gtggacagcc cccgaggcag ccctctatgg ccggttcacc atcaagtcgg
     1381 atgtctggtc cttcggcatc ctgctgactg agctgaccac caagggccgg gtgccatacc
     1441 cagggatggt caacagggag gtgctggacc aggtggagag gggctaccgc atgccctgcc
     1501 cgcccgagtg ccccgagtcg ctgcatgacc tcatgtgcca gtgctggcgg aaggaccctg
     1561 aggagcggcc cacttttgag tacctgcagg ccttcctgga ggactacttc acctcgacag
     1621 agccccagta ccagcctgga gagaacctac gggatccacc ggtcgccacc atgagtgcga
     1681 ttaagccaga catgaagatc aaactccgta tggaaggcaa cgtaaacggg caccactttg
     1741 tgatcgacgg agatggtaca ggcaagcctt ttgagggaaa acagagtatg gatcttgaag
     1801 tcaaagaggg cggacctctg ccttttgcct ttgatatcct gaccactgca ttcctgtacg
     1861 gcaacagggt attcgccaaa tatccagaca acatacaaga ctattttaag cagtcgtttc
     1921 ctaaggggta ttcgtgggaa cgaagcttga ctttcgaaga cgggggcatt tgcaacgcca
     1981 gaaacgacat aacaatggaa ggggacactt tctataataa agttcgattt tatggtacca
     2041 actttcccgc caatggtcca gttatgcaga agaagacgct gaaatgggag ccctccactg
     2101 agaaaatgta tgtgcgtgat ggagtgctga cgggtgatgt agagatggct ttgttgcttg
     2161 aaggaaatgc ccattaccga tgtgacttca gaactactta caaagctaag gagaagggtg
     2221 tcaagttacc aggcgcccac tttgtggacc actgcattga gattttaagc catgacaaag
     2281 attacaacaa ggttaagctg tatgagcatg ctgttgctca ttctggattg cctgacaatg
     2341 ccagacgata agcggccgcg actctagatc ataatcagcc ataccac
//
