LOCUS       sequence-88693-e        1159 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Gap Junctions, Excitation: 503 / 569, Emission: 514 / 
            581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1159)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 1159)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mGeos2-VEL-Cx43-7
FEATURES             Location/Qualifiers
     source          1..1159
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             422..1102
                     /codon_start=1
                     /product="truly monomeric reversibly photoswitchable 
                     fluorescent protein, suitable for nonlinear structured 
                     illumination microscopy (Zhang et al., 2016)"
                     /label=Skylan-NS
                     /note="mEos3.1 derivative that eliminates the green-to-red 
                     conversion"
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFLYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDVEMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
ORIGIN
        1 ttacatggcc accactgcca ctgagcccat tcaaagactg cgatctcaaa atacgcctac
       61 tcaatgctgc tcctcaccaa cggctcactc tcgcctatgt ctctcctggg tacaagctgg
      121 ttactggtga cagaaacaat tcctcgtgcc gcaattacac aagcaagcta gcgagcaaaa
      181 ctgggcgaac tacagcgcag agcaaaatcg catggggcag gccggaagca ccatctccaa
      241 ctcgcacgcc cagccgttcg atttccccga cgacaaccag aatgccaaaa aagttgctgc
      301 tggacatgaa ctccagccat tagccatcgt ggaccaacga ccttccagca gagccagcag
      361 ccgcgccagc agcaggcctc ggcctgatga cctggagatt gcggatccac cggtcgccac
      421 catgagtgcg attaagccag acatgaagat caaactccgt atggaaggca acgtaaacgg
      481 gcaccacttt gtgatcgacg gagatggtac aggcaagcct tttgagggaa aacagagtat
      541 ggatcttgaa gtcaaagagg gcggacctct gccttttgcc tttgatatcc tgaccactgc
      601 attcctgtac ggcaacaggg tattcgccaa atatccagac aacatacaag actattttaa
      661 gcagtcgttt cctaaggggt attcgtggga acgaagcttg actttcgaag acgggggcat
      721 ttgcaacgcc agaaacgaca taacaatgga aggggacact ttctataata aagttcgatt
      781 ttatggtacc aactttcccg ccaatggtcc agttatgcag aagaagacgc tgaaatggga
      841 gccctccact gagaaaatgt atgtgcgtga tggagtgctg acgggtgatg tagagatggc
      901 tttgttgctt gaaggaaatg cccattaccg atgtgacttc agaactactt acaaagctaa
      961 ggagaagggt gtcaagttac caggcgccca ctttgtggac cactgcattg agattttaag
     1021 ccatgacaaa gattacaaca aggttaagct gtatgagcat gctgttgctc attctggatt
     1081 gcctgacaat gccagacgat aagcggccgc gactctagat cataatcagc catacccaca
     1141 ttgtaggaag ttttgccca
//
