LOCUS       sequence-88694-e         923 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane, Excitation: 503 / 569, Emission: 514 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 923)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mGeos2-VEL-Farnesyl-5
FEATURES             Location/Qualifiers
     source          1..923
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             47..724
                     /codon_start=1
                     /product="truly monomeric reversibly photoswitchable 
                     fluorescent protein, suitable for nonlinear structured 
                     illumination microscopy (Zhang et al., 2016)"
                     /label=Skylan-NS
                     /note="mEos3.1 derivative that eliminates the green-to-red 
                     conversion"
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFLYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDVEMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
ORIGIN
        1 ccaatggcta gtgacgtcag atccgctagc gctaccggtc gccaccatga gtgcgattaa
       61 gccagacatg aagatcaaac tccgtatgga aggcaacgta aacgggcacc actttgtgat
      121 cgacggagat ggtacaggca agccttttga gggaaaacag agtatggatc ttgaagtcaa
      181 agagggcgga cctctgcctt ttgcctttga tatcctgacc actgcattcc tgtacggcaa
      241 cagggtattc gccaaatatc cagacaacat acaagactat tttaagcagt cgtttcctaa
      301 ggggtattcg tgggaacgaa gcttgacttt cgaagacggg ggcatttgca acgccagaaa
      361 cgacataaca atggaagggg acactttcta taataaagtt cgattttatg gtaccaactt
      421 tcccgccaat ggtccagtta tgcagaagaa gacgctgaaa tgggagccct ccactgagaa
      481 aatgtatgtg cgtgatggag tgctgacggg tgatgtagag atggctttgt tgcttgaagg
      541 aaatgcccat taccgatgtg acttcagaac tacttacaaa gctaaggaga agggtgtcaa
      601 gttaccaggc gcccactttg tggaccactg cattgagatt ttaagccatg acaaagatta
      661 caacaaggtt aagctgtatg agcatgctgt tgctcattct ggattgcctg acaatgccag
      721 acgatccgga ctcagatcta agctgaaccc tcctgatgag agtggccccg gctgcatgag
      781 ctgcaagtgt gtgctctcct gagaattctg cagtcgacgg taccgcgggc ccgggatcca
      841 ccggatctag ataactgatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt
      901 taaaaaacct cccacacctc ccc
//
