LOCUS       sequence-88698-e        1852 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Microtubules, Excitation: 503 / 569, Emission: 514 / 
            581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1852)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 1852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mGeos2-VEL-MAPTau-N-10
FEATURES             Location/Qualifiers
     source          1..1852
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      39..48
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1131..1811
                     /codon_start=1
                     /product="truly monomeric reversibly photoswitchable 
                     fluorescent protein, suitable for nonlinear structured 
                     illumination microscopy (Zhang et al., 2016)"
                     /label=Skylan-NS
                     /note="mEos3.1 derivative that eliminates the green-to-red 
                     conversion"
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFLYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDVEMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
ORIGIN
        1 cgtcagatcc gctagcgcta ccggactcag atctcgaggc caccatggct gagccccgcc
       61 aggagttcga agtgatggaa gatcacgctg ggacgtacgg gttgggggac aggaaagatc
      121 aggggggcta caccatgcac caagaccaag agggtgacac ggacgctggc ctgaaagctg
      181 aagaagcagg cattggagac acccccagcc tggaagacga agctgctggt cacgtgaccc
      241 aagctcgcat ggtcagtaaa agcaaagacg ggactggaag cgatgacaaa aaagccaagg
      301 gggctgatgg taaaacgaag atcgccacac cgcggggagc agcccctcca ggccagaagg
      361 gccaggccaa cgccaccagg attccagcaa aaaccccgcc cgctccaaag acaccaccca
      421 gctctggtga acctccaaaa tcaggggatc gcagcggcta cagcagcccc ggctccccag
      481 gcactcccgg cagccgctcc cgcaccccgt cccttccaac cccacccacc cgggagccca
      541 agaaggtggc agtggtccgt actccaccca agtcgccgtc ttccgccaag agccgcctgc
      601 agacagcccc cgtgcccatg ccagacctga agaatgtcaa gtccaagatc ggctccactg
      661 agaacctgaa gcaccagccg ggaggcggga aggtgcaaat agtctacaaa ccagttgacc
      721 tgagcaaggt gacctccaag tgtggctcat taggcaacat ccatcataaa ccaggaggtg
      781 gccaggtgga agtaaaatct gagaagcttg acttcaagga cagagtccag tcgaagattg
      841 ggtccctgga caatatcacc cacgtccctg gcggaggaaa taaaaagatt gaaacccaca
      901 agctgacctt ccgcgagaac gccaaagcca agacagacca cggggcggag atcgtgtaca
      961 agtcgccagt ggtgtctggg gacacgtctc cacggcatct cagcaatgtc tcctccaccg
     1021 gcagcatcga catggtagac tcgccccagc tcgccacgct agctgacgag gtgtctgcct
     1081 ccctggccaa gcagggtttg agctccggag gggatccacc ggtcgccacc atgagtgcga
     1141 ttaagccaga catgaagatc aaactccgta tggaaggcaa cgtaaacggg caccactttg
     1201 tgatcgacgg agatggtaca ggcaagcctt ttgagggaaa acagagtatg gatcttgaag
     1261 tcaaagaggg cggacctctg ccttttgcct ttgatatcct gaccactgca ttcctgtacg
     1321 gcaacagggt attcgccaaa tatccagaca acatacaaga ctattttaag cagtcgtttc
     1381 ctaaggggta ttcgtgggaa cgaagcttga ctttcgaaga cgggggcatt tgcaacgcca
     1441 gaaacgacat aacaatggaa ggggacactt tctataataa agttcgattt tatggtacca
     1501 actttcccgc caatggtcca gttatgcaga agaagacgct gaaatgggag ccctccactg
     1561 agaaaatgta tgtgcgtgat ggagtgctga cgggtgatgt agagatggct ttgttgcttg
     1621 aaggaaatgc ccattaccga tgtgacttca gaactactta caaagctaag gagaagggtg
     1681 tcaagttacc aggcgcccac tttgtggacc actgcattga gattttaagc catgacaaag
     1741 attacaacaa ggttaagctg tatgagcatg ctgttgctca ttctggattg cctgacaatg
     1801 ccagacgata agcggccgcg actctagatc ataatcagcc ataccacatt gt
//
