LOCUS       sequence-88699-e        6699 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytoskeleton, Excitation: 503 / 569, Emission: 514 / 
            581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6699)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 6699)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mGeos2-VEL-MyosinIIA-C-18
FEATURES             Location/Qualifiers
     source          1..6699
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             31..708
                     /codon_start=1
                     /product="truly monomeric reversibly photoswitchable 
                     fluorescent protein, suitable for nonlinear structured 
                     illumination microscopy (Zhang et al., 2016)"
                     /label=Skylan-NS
                     /note="mEos3.1 derivative that eliminates the green-to-red 
                     conversion"
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFLYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDVEMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
ORIGIN
        1 ctcgagccgc tagcgctacc ggtcgccacc atgagtgcga ttaagccaga catgaagatc
       61 aaactccgta tggaaggcaa cgtaaacggg caccactttg tgatcgacgg agatggtaca
      121 ggcaagcctt ttgagggaaa acagagtatg gatcttgaag tcaaagaggg cggacctctg
      181 ccttttgcct ttgatatcct gaccactgca ttcctgtacg gcaacagggt attcgccaaa
      241 tatccagaca acatacaaga ctattttaag cagtcgtttc ctaaggggta ttcgtgggaa
      301 cgaagcttga ctttcgaaga cgggggcatt tgcaacgcca gaaacgacat aacaatggaa
      361 ggggacactt tctataataa agttcgattt tatggtacca actttcccgc caatggtcca
      421 gttatgcaga agaagacgct gaaatgggag ccctccactg agaaaatgta tgtgcgtgat
      481 ggagtgctga cgggtgatgt agagatggct ttgttgcttg aaggaaatgc ccattaccga
      541 tgtgacttca gaactactta caaagctaag gagaagggtg tcaagttacc aggcgcccac
      601 tttgtggacc actgcattga gattttaagc catgacaaag attacaacaa ggttaagctg
      661 tatgagcatg ctgttgctca ttctggattg cctgacaatg ccagacgatc cggactcaga
      721 tctggcagcg gtggaggcag cgcatccggc ggaagcggaa gcgctcagca ggctgcagac
      781 aagtacctct atgtggataa aaacttcatc aataacccgc tggcccaagc tgactgggct
      841 gccaagaagt tggtatgggt gccttccagc aagaatggct ttgaaccagc cagcctcaag
      901 gaggaggtgg gagaagaggc cattgtagag ctggtagaga atgggaagaa ggtgaaggtg
      961 aacaaggacg acatccagaa gatgaaccca cccaagttct ccaaggtgga ggacatggca
     1021 gagctcacgt gcctcaacga agcttcggtg ctgcacaacc tcaaggagcg atactactca
     1081 gggcttatct acacctattc aggcctgttc tgtgtggtca tcaaccctta taagaacctg
     1141 cccatctact cagaggagat cgtggagatg tacaagggca agaagaggca cgagatgcca
     1201 ccccacatct acgccatcac agatactgcc taccggagca tgatgcagga ccgggaagat
     1261 cagtccatcc tgtgcacggg ggagtctgga gcagggaaga cagagaacac caagaaagtc
     1321 atccagtacc tggcacatgt ggcctcctca cacaagagca agaaggacca gggggagttg
     1381 gagcggcagc tgctacaggc caaccctatc ctagaggcct ttggaaacgc caagacggtg
     1441 aagaatgaca actcctctcg attcggtaaa ttcattcgta tcaactttga tgtcaatggc
     1501 tacattgttg gtgccaacat tgagacttat cttctggaga aatctcgtgc tatccgccaa
     1561 gccaaagagg agcggacctt ccacatcttc tactacctgc tgtctggggc cggagaacac
     1621 ctgaagactg atctcctgtt ggagccatac aacaaatacc gcttcctgtc caacgggcac
     1681 gtcaccatcc ctgggcagca ggacaaggac atgttccagg agacaatgga ggccatgaga
     1741 attatgggta tcccagagga tgagcagatg ggcttgctgc gggtcatctc tggggtcctt
     1801 cagcttggca acattgcctt caagaaggag cggaacactg accaggcgtc catgccggac
     1861 aacacagctg ctcaaaaggt gtcccacctc ctggggatca atgtgaccga cttcaccaga
     1921 ggcatcctca ccccacgcat caaggtgggc agagactatg tgcagaaggc gcagactaaa
     1981 gagcaggctg actttgccat tgaggccttg gccaaggcta cctatgagcg gatgttccgc
     2041 tggctggtgc ttcgcatcaa caaagctctg gacaagacca agaggcaggg cgcctcattt
     2101 atcgggatac tggacatcgc tggctttgaa atctttgatc tgaactcctt cgagcagctg
     2161 tgcatcaact acaccaacga gaagctgcag cagctgttca accacaccat gttcatcctg
     2221 gagcaggagg agtaccagcg agagggcatc gagtggaact tcatcgactt cggcctggac
     2281 ctgcagccct gcatcgacct cattgagaag ccggcgggtc ccccaggcat cctggccctg
     2341 ctagatgagg agtgctggtt tcctaaggcc actgacaaga gcttcgtgga gaaggtggtg
     2401 caggagcagg gcacccaccc caagttccag aagcccaagc aactgaagga caaggctgat
     2461 ttctgcatta tccactatgc cggcaaggtg gactataaag ctgacgagtg gctgatgaag
     2521 aacatggacc ccttgaacga caacatcgcc acgctgcttc accagtcctc agacaagttt
     2581 gtctctgagc tgtggaagga tgtggatcgg atcattggct tggaccaagt ggctggaatg
     2641 tccgagacag cactacctgg tgccttcaag acccggaagg gcatgttccg tactgtcgga
     2701 cagctgtaca aggagcagct ggccaagctc atggccacgt tgaggaatac caaccccaac
     2761 ttcgtgcgct gcatcattcc caaccatgag aagaaggccg gcaaactgga cccgcacttg
     2821 gtgctggacc agctgcgctg caatggcgtc cttgagggca tccggatctg ccgccagggc
     2881 tttcccaaca gggtggtctt ccaggagttc cggcagaggt atgagatcct cacccccaac
     2941 tccatcccga agggcttcat ggatggcaag caagcgtgtg tgctcatgat caaagccttg
     3001 gagcttgaca gcaacctgta ccgcatcggc cagagcaaag tgttcttccg ggcaggagtg
     3061 ctcgcccacc tggaggaaga gcgggacctg aagatcaccg atgtcatcat tggcttccag
     3121 gcctgctgca ggggctacct ggccaggaag gcctttgcca agaggcagca acagctgacc
     3181 gccatgaagg tcctacagag gaactgtgct gcgtacctca ggctgcgcaa ctggcagtgg
     3241 tggaggctct tcaccaaggt caagcccctg ttgaactcaa taagacatga ggatgagctg
     3301 ttagccaagg aggcggaact gacaaaggtt cgagagaaac atctggctgc agagaacagg
     3361 ctgacagaga tggagacgat gcagtctcag ctcatggcag agaagctgca gcttcaggag
     3421 cagctgcagg cggagacaga gctgtgtgcc gaggctgagg agctccgggc ccgtctgaca
     3481 gcgaagaagc aggagctgga ggagatatgc catgacctgg aggccagggt ggaggaggag
     3541 gaggagcgct gccagtacct gcaggccgag aagaagaaga tgcagcagaa catccaggaa
     3601 cttgaggagc agttggagga ggaggagagc gcccggcaga agctgcagct tgagaaggtg
     3661 accaccgagg ccaagctgaa gaaactggag gaggaccaga tcatcatgga ggaccagaac
     3721 tgcaaactgg ccaaggagaa gaaactgctg gaagacagag tagctgagtt cactaccaac
     3781 ctcatggaag aggaggagaa gtccaagagc ctggccaagc tcaagaacaa gcacgaggca
     3841 atgatcaccg acctggaaga gcgcctccgt agggaggaga agcagaggca ggagttggag
     3901 aagacccgtc gcaagctgga gggagactcc acagacctca gtgaccagat tgctgagctc
     3961 caggcgcaga tagcagagct caagatgcag ctggccaaga aggaggagga gttgcaggct
     4021 gccttggcca gagtggaaga agaagctgct cagaagaata tggccctgaa gaagatccga
     4081 gaactggaaa ctcagatatc tgagctccag gaggacctgg agtcggagcg agcctccagg
     4141 aataaagccg agaagcagaa acgggatctg ggagaggagc tggaggcgct gaagacagag
     4201 ctggaggaca cgctggactc cacggctgcc cagcaggagc tgaggtcgaa gcgtgagcag
     4261 gaggtgagca tcctgaagaa gactctggag gacgaggcca agacccatga ggcccagatc
     4321 caggagatga ggcagaagca ctcacaggct gtggaggagc tggcagatca gttggagcag
     4381 acgaagcggg taaaagctac ccttgagaag gcgaagcaga ccctggagaa tgagcgggga
     4441 gagctggcca atgaggtgaa ggccctgctg caaggcaagg gcgactcaga gcacaagcgc
     4501 aagaaggtgg aggcgcagct gcaagaactg caggtcaagt tcagcgaggg agagcgcgtg
     4561 cgaaccgaac tggccgacaa ggtcaccaag ctgcaggttg aactggacag tgtgaccggt
     4621 ctccttagcc agtctgacag caagtccagc aagcttacga aggacttctc tgcgctggag
     4681 tcccagcttc aggacacaca ggagttgctc caggaggaga accggcagaa gctgagcctg
     4741 agcaccaagc tcaagcagat ggaggatgag aaaaactcct tcagggagca gctggaggag
     4801 gaggaggagg ccaagcgcaa cttggagaag cagatcgcca cgctccatgc ccaggtgacc
     4861 gacatgaaga agaagatgga ggacggtgta gggtgcctgg agactgcaga ggaggcgaag
     4921 cggaggcttc agaaggactt ggaaggcctg agccagcggc ttgaggagaa ggtggctgcc
     4981 tacgataagc tggagaagac caagacacgg ctgcagcagg agctggacga cctgctggtt
     5041 gacctggacc accagcggca gagcgtctcc aacctggaaa agaagcagaa gaagttcgac
     5101 cagctcctag ccgaggagaa gaccatctcg gccaagtatg cagaggagcg tgaccgagct
     5161 gaggctgagg cccgtgagaa ggagacaaag gcgctatcac tggcccgggc gcttgaggag
     5221 gccatggagc agaaggcaga gctggagcgg ctcaacaagc agttccgcac ggagatggag
     5281 gacctcatga gctccaagga tgacgtgggc aagagtgtcc acgagctgga gaagtccaag
     5341 cgggccttgg agcagcaggt ggaggagatg aagacccagc tggaggagct ggaggatgag
     5401 ctgcaggcca cggaggatgc caagctccgc ctggaggtga acctgcaggc catgaaggcc
     5461 cagtttgagc gggatctgca gggccgggat gaacagagcg aggagaagaa gaagcagctg
     5521 gtcagacagg tgcgggagat ggaggcggag ctggaggatg agaggaagca gcgctccatg
     5581 gccatggccg cacgcaagaa actggagatg gatctgaagg acctggaggc acacattgac
     5641 acagccaata agaaccggga agaggccatc aaacagctgc ggaagcttca ggcccagatg
     5701 aaggactgca tgcgggagct ggacgacacg cgcgcctccc gggaggagat cctggcgcag
     5761 gccaaggaga atgagaagaa gctgaagagc atggaggccg agatgattca gctgcaggag
     5821 gaactggcag ctgctgagcg tgctaagcgt caggcccaac aggaacggga cgagctggct
     5881 gatgagatcg ccaacagcag tggcaaaggg gccctagcat tagaggagaa gcggcgactg
     5941 gaggcccgca ttgccctgct ggaggaggag ctggaggagg aacagggcaa cacggagctg
     6001 atcaacgatc ggctgaagaa ggccaacctg cagatcgacc aaataaacac cgacctgaac
     6061 ctggaacgca gccacgcaca gaagaatgag aatgcgcgac agcagctgga acgccagaac
     6121 aaggagctca aggccaagct gcaggaaatg gagagtgctg tcaagtccaa atacaaggcc
     6181 tccatcgcgg ccttggaggc caaaattgca cagctggagg aacagctgga caacgagacc
     6241 aaggagcgcc aggcagcctc caagcaggtg cgccggacgg agaagaagct gaaggacgtg
     6301 ctgctgcagg tggaggacga gcggaggaac gcggaacagt tcaaggacca ggctgacaag
     6361 gcgtccaccc gcctgaagca gcttaaacgg cagctagagg aggctgaaga ggaggcccag
     6421 cgggccaatg cctcacgccg gaagctgcag cgtgagctgg aagatgccac agagaccgct
     6481 gatgctatga accgcgaggt cagctccctg aagaacaaac tgaggcgtgg ggacctgcca
     6541 tttgtcgtga ctcgccgaat tgttcggaaa ggcactggcg actgctcaga cgaggaggtc
     6601 gacggtaaag cagatggggc cgatgccaag gcagctgaat aggaattctg cagtcgacgg
     6661 taccgcgggc ccgggatcca ccggatctag ataactgat
//
