LOCUS       sequence-88701-e        2487 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 503 / 569, Emission: 514 
            / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2487)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 2487)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mGeos2-VEL-Paxillin-22
FEATURES             Location/Qualifiers
     source          1..2487
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    1704..1756
                     /label=MCS
                     /note="multiple cloning site"
     CDS             1770..2450
                     /codon_start=1
                     /product="truly monomeric reversibly photoswitchable 
                     fluorescent protein, suitable for nonlinear structured 
                     illumination microscopy (Zhang et al., 2016)"
                     /label=Skylan-NS
                     /note="mEos3.1 derivative that eliminates the green-to-red 
                     conversion"
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFLYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDVEMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
ORIGIN
        1 agtggaacgt cagatccgct agcaccatgg acgacctcga tgccttactg gcagacctgg
       61 aatccaccac ctcgcatatc tctaagcgac cggtgtttct aacagaggaa acgccttact
      121 cctacccaac tggaaaccat acgtaccagg agattgctgt gccaccgccg gtgcccccgc
      181 cgccttccag tgaggcccta aatggcactg tgattgaccc cttagaccag tggcaaccca
      241 gcgtgtccag atacggccac cagcaacctc agtcccagtc tccgatatac agctctagtg
      301 ccaaaagctc cagtgcctct gttcctagag acgggctcag ctccccttct ccccgtgcca
      361 gtgaggagga acacgtgtac agcttcccaa acaagcagaa gtctgcagaa ccatctccca
      421 caatgaccag cacctccctg ggcagcaacc tctcggaact ggacaggctt ctcctggaac
      481 tgaatgctgt tcaacataat ccccccagtg gcttctcagc agatgaagtc agcagaagcc
      541 catcactgcc caatgtgacc ggacctcact atgtcatccc agagagcagc agctctgcag
      601 gagggaaggc tgcaccccct acaaaagaaa aaccaaagcg gaatggtggg cgtgggattg
      661 aagatgtgcg tcccagtgtg gagagcctgc tggatgagct ggagagctct gtgccaagcc
      721 cagtccctgc aatcactgtg agccaagggg aggtgagcag cccccagcgt gtcaacgcca
      781 gtcagcagca gacccgtatc tctgcttctt cagctacacg agaactggat gagctgatgg
      841 cgtccctctc tgactttaag ttcatggcac aggggaaagc cggcggcagc agctccccac
      901 catccaccac cccaaagcct ggcagtcagc tggacaccat gctgggaagt ctgcagtctg
      961 acctgaacaa actgggggta gcgacggtcg ccaagggtgt gtgcggagcc tgcaagaagc
     1021 cgattgctgg gcaggtagtt acagccatgg ggaagacctg gcaccctgag cacttcgtct
     1081 gcacccactg ccaggaggag attggatcac ggaacttctt tgagcgggat gggcagccct
     1141 actgtgagaa ggactatcac aacctcttct ctcctcgctg ctactactgc aatgggccaa
     1201 tccttgataa agtggtgacg gctttggaca ggacgtggca ccctgaacac tttttctgtg
     1261 cccagtgtgg agttttcttt ggacctgaag gatttcatga gaaggatggc aaagcctatt
     1321 gccgcaagga ctactttgac atgtttgctc ccaagtgtgg aggctgtgcc cgggctatcc
     1381 tggaaaatta catttctgcc ttaaacaccc tgtggcaccc tgagtgcttt gtctgtcggg
     1441 aatgtttcac accgttcatc aacggcagct tcttcgagca cgacggccag ccctactgcg
     1501 aggtgcatta ccacgagcgc cgcggctcgc tgtgctctgg ctgccagaag cccatcacgg
     1561 gacgctgcat cactgccatg ggcaagaaat ttcaccccga acactttgtc tgtgccttct
     1621 gcctcaagca gctcaacaaa ggaaccttca aagaacagaa cgacaagccc tactgtcaga
     1681 actgctttct caaactcttc tgtctcgagc tcaagcttcg aattctgcag tcgacggtac
     1741 cgcgggcccg ggatccaccg gtcgccacca tgagtgcgat taagccagac atgaagatca
     1801 aactccgtat ggaaggcaac gtaaacgggc accactttgt gatcgacgga gatggtacag
     1861 gcaagccttt tgagggaaaa cagagtatgg atcttgaagt caaagagggc ggacctctgc
     1921 cttttgcctt tgatatcctg accactgcat tcctgtacgg caacagggta ttcgccaaat
     1981 atccagacaa catacaagac tattttaagc agtcgtttcc taaggggtat tcgtgggaac
     2041 gaagcttgac tttcgaagac gggggcattt gcaacgccag aaacgacata acaatggaag
     2101 gggacacttt ctataataaa gttcgatttt atggtaccaa ctttcccgcc aatggtccag
     2161 ttatgcagaa gaagacgctg aaatgggagc cctccactga gaaaatgtat gtgcgtgatg
     2221 gagtgctgac gggtgatgta gagatggctt tgttgcttga aggaaatgcc cattaccgat
     2281 gtgacttcag aactacttac aaagctaagg agaagggtgt caagttacca ggcgcccact
     2341 ttgtggacca ctgcattgag attttaagcc atgacaaaga ttacaacaag gttaagctgt
     2401 atgagcatgc tgttgctcat tctggattgc ctgacaatgc cagacgataa gcggccgcga
     2461 ctctagatca taatcagcca taccaca
//
