LOCUS       sequence-88702-e        1941 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Mitochondria, Excitation: 503 / 569, Emission: 514 / 
            581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1941)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 1941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mGeos2-VEL-PDHA1-N-10
FEATURES             Location/Qualifiers
     source          1..1941
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1227..1907
                     /codon_start=1
                     /product="truly monomeric reversibly photoswitchable 
                     fluorescent protein, suitable for nonlinear structured 
                     illumination microscopy (Zhang et al., 2016)"
                     /label=Skylan-NS
                     /note="mEos3.1 derivative that eliminates the green-to-red 
                     conversion"
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFLYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDVEMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
ORIGIN
        1 acgtcagatc cgctagcacc gccaccatga ggaagatgct cgccgccgtc tcccgcgtgc
       61 tgtctggcgc ttctcagaag ccggcaagca gagtgctggt agcatcccgt aattttgcaa
      121 atgatgctac atttgaaatt aagaaatgtg accttcaccg gctggaagaa ggccctcctg
      181 tcacaacagt gctcaccagg gaggatgggc tcaaatacta caggatgatg cagactgtac
      241 gccgaatgga gttgaaagca gatcagctgt ataaacagaa aattattcgt ggtttctgtc
      301 acttgtgtga tggtcaggaa gcttgctgtg tgggcctgga ggccggcatc aaccccacag
      361 accatctcat cacagcctac cgggctcacg gctttacttt cacccggggc ctttccgtcc
      421 gagaaattct cgcagagctt acaggacgaa aaggaggttg tgctaaaggg aaaggaggat
      481 cgatgcacat gtatgccaag aacttctacg ggggcaatgg catcgtggga gcgcaggtgc
      541 ccctgggcgc tgggattgct ctagcctgta agtataatgg aaaagatgag gtctgcctga
      601 ctttatatgg cgatggtgct gctaaccagg gccagatatt cgaagcttac aacatggcag
      661 ctttgtggaa attaccttgt attttcatct gtgagaataa tcgctatgga atgggaacgt
      721 ctgttgagag agcggcagcc agcactgatt actacaagag aggcgatttc attcctgggc
      781 tgagagtgga tggaatggat atcctgtgcg tccgagaggc aacaaggttt gctgctgcct
      841 attgtagatc tgggaagggg cccatcctga tggagctgca gacttaccgt taccacggac
      901 acagtatgag tgaccctgga gtcagttacc gtacacgaga agaaattcag gaagtaagaa
      961 gtaagagtga ccctattatg cttctcaagg acaggatggt gaacagcaat cttgccagtg
     1021 tggaagaact aaaggaaatt gatgtggaag tgaggaagga gattgaggat gctgcccagt
     1081 ttgccacggc cgatcctgag ccacctttgg aagagctggg ctaccacatc tactccagcg
     1141 acccaccttt tgaagttcgt ggtgccaatc agtggatcaa gtttaagtca gtcagtagct
     1201 ccggagggga tccaccggtc gccaccatga gtgcgattaa gccagacatg aagatcaaac
     1261 tccgtatgga aggcaacgta aacgggcacc actttgtgat cgacggagat ggtacaggca
     1321 agccttttga gggaaaacag agtatggatc ttgaagtcaa agagggcgga cctctgcctt
     1381 ttgcctttga tatcctgacc actgcattcc tgtacggcaa cagggtattc gccaaatatc
     1441 cagacaacat acaagactat tttaagcagt cgtttcctaa ggggtattcg tgggaacgaa
     1501 gcttgacttt cgaagacggg ggcatttgca acgccagaaa cgacataaca atggaagggg
     1561 acactttcta taataaagtt cgattttatg gtaccaactt tcccgccaat ggtccagtta
     1621 tgcagaagaa gacgctgaaa tgggagccct ccactgagaa aatgtatgtg cgtgatggag
     1681 tgctgacggg tgatgtagag atggctttgt tgcttgaagg aaatgcccat taccgatgtg
     1741 acttcagaac tacttacaaa gctaaggaga agggtgtcaa gttaccaggc gcccactttg
     1801 tggaccactg cattgagatt ttaagccatg acaaagatta caacaaggtt aagctgtatg
     1861 agcatgctgt tgctcattct ggattgcctg acaatgccag acgataagcg gccgcgactc
     1921 tagatcataa tcagccatac c
//
