LOCUS       sequence-88704-e         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Golgi TGN, Excitation: 503 / 569, Emission: 514 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mGeos2-VEL-SiT-N-15
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             229..909
                     /codon_start=1
                     /product="truly monomeric reversibly photoswitchable 
                     fluorescent protein, suitable for nonlinear structured 
                     illumination microscopy (Zhang et al., 2016)"
                     /label=Skylan-NS
                     /note="mEos3.1 derivative that eliminates the green-to-red 
                     conversion"
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFLYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDVEMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
ORIGIN
        1 acgtcagatc cgctagcgct accggactca gatctcgagc tcgccaccat gattcacacc
       61 aacctgaaga aaaagttcag ctgctgcgtc ctggtctttc ttctgtttgc agtcatctgt
      121 gtgtggaagg aaaagaagaa agggagttac tatgattcct ttaaattgca aaccaaggaa
      181 ttcctgcagt cgacggtacc gcgggcccgg gatccaccgg tcgccaccat gagtgcgatt
      241 aagccagaca tgaagatcaa actccgtatg gaaggcaacg taaacgggca ccactttgtg
      301 atcgacggag atggtacagg caagcctttt gagggaaaac agagtatgga tcttgaagtc
      361 aaagagggcg gacctctgcc ttttgccttt gatatcctga ccactgcatt cctgtacggc
      421 aacagggtat tcgccaaata tccagacaac atacaagact attttaagca gtcgtttcct
      481 aaggggtatt cgtgggaacg aagcttgact ttcgaagacg ggggcatttg caacgccaga
      541 aacgacataa caatggaagg ggacactttc tataataaag ttcgatttta tggtaccaac
      601 tttcccgcca atggtccagt tatgcagaag aagacgctga aatgggagcc ctccactgag
      661 aaaatgtatg tgcgtgatgg agtgctgacg ggtgatgtag agatggcttt gttgcttgaa
      721 ggaaatgccc attaccgatg tgacttcaga actacttaca aagctaagga gaagggtgtc
      781 aagttaccag gcgcccactt tgtggaccac tgcattgaga ttttaagcca tgacaaagat
      841 tacaacaagg ttaagctgta tgagcatgct gttgctcatt ctggattgcc tgacaatgcc
      901 agacgataag cggccgcgac tctagatcat aatcagccat accacatttg tagaggtttt
      961 acttgcttta aaaacctccc acacctcccc ctga
//
