LOCUS       sequence-88712-e        1117 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Mitochondria, Excitation: 505 / 569, Emission: 516 / 
            581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1117)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 1117)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: dEos-Mito-3
FEATURES             Location/Qualifiers
     source          1..1117
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        60..159
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     110..130
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             188..262
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             284..964
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="MSAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
ORIGIN
        1 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt tgtttggcac
       61 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc
      121 ggtaggcgtg tacggtggga ggtctatata agcagagctg gtttagtgaa ccgtcagatc
      181 cgctagcatg tccgtcctga cgccgctgct gctgcggggc ttgacaggct cggcccggcg
      241 gctcccagtg ccgcgcgcca agatccattc gttgggggat ccaatgagtg cgattaagcc
      301 agacatgaag atcaacctcc gtatggaagg caacgtaaac gggcaccact ttgtgatcga
      361 cggagatggt acaggcaagc cttttgaggg aaaacagagt atggatcttg aagtcaaaga
      421 gggcggacct ctgccttttg cctttgatat cctgaccact gcattccatt acggcaacag
      481 ggtattcgcc gaatatccag accacataca agactatttt aagcagtcgt ttcctaaggg
      541 gtattcgtgg gaacgaagct tgactttcga agacgggggc atttgcattg ccagaaacga
      601 cataacaatg gaaggggaca ctttctataa taaagttcga tttcacggtg taaactttcc
      661 cgccaatggt ccagttatgc agaagaagac gctgaaatgg gagccctcca ctgagaaaat
      721 gtatgtgcgt gatggagtgc tgacgggtga tattcacatg gctttgttgc ttgaaggaaa
      781 tgcccattac cgatgtgact tcagaactac ttacaaagct aaggagaagg gtgtcaagtt
      841 accaggctac cactttgtgg accactgcat tgagatttta agccatgaca aagattacaa
      901 caaggttaag ctgtatgagc atgctgttgc tcattctgga ttgcctgaca atgccagacg
      961 ataagcggcc gcgactctag atcataatca gcataccaca tttgtagagg tttacttgct
     1021 taaaaacctc cacacctccc cctgacctga acataaatga tgcattgtgt gtacttgtta
     1081 tgcactatat ggtacaataa gcatacatca caattca
//
