LOCUS       sequence-88715-e        3979 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 
            / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3979)
  AUTHORS   Betzig E, Patterson GH, Sougrat R, Lindwasser OW, Olenych S, 
            Bonifacino JS, Davidson MW, Lippincott-Schwartz J, Hess HF
  TITLE     Imaging intracellular fluorescent proteins at nanometer resolution.
  JOURNAL   Science. 2006 Sep 15;313(5793):1642-5. Epub 2006 Aug 10.
  PUBMED    16902090
REFERENCE   2  (bases 1 to 3979)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2006-09-15"; volume: "313(5793)"; pages: "1642-5. Epub 2006 
            Aug 10"
COMMENT     Sequence Label: dEos-Vinculin-14
FEATURES             Location/Qualifiers
     source          1..3979
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      19..28
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             21..698
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="MVAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
ORIGIN
        1 acgtcagatc cgctagcacc atggtggcga ttaagccaga catgaagatc aacctccgta
       61 tggaaggcaa cgtaaacggg caccactttg tgatcgacgg agatggtaca ggcaagcctt
      121 ttgagggaaa acagagtatg gatcttgaag tcaaagaggg cggacctctg ccttttgcct
      181 ttgatatcct gaccactgca ttccattacg gcaacagggt attcgccgaa tatccagacc
      241 acatacaaga ctattttaag cagtcgtttc ctaaggggta ttcgtgggaa cgaagcttga
      301 ctttcgaaga cgggggcatt tgcattgcca gaaacgacat aacaatggaa ggggacactt
      361 tctataataa agttcgattt cacggtgtaa actttcccgc caatggtcca gttatgcaga
      421 agaagacgct gaaatgggag ccctccactg agaaaatgta tgtgcgtgat ggagtgctga
      481 ccggtgatat tcgcatggct ttgttgcttg aaggaaatgc ccattaccga tgtgacttca
      541 gaactactta caaagctaag gagaagggtg tcaagttacc aggctaccac tttgtggacc
      601 actgcattga gattttaagc catgacaaag attacaacaa ggttaagctg tatgagcatg
      661 ctgttgctca ttctggattg cctgacaatg ccagacgaga gaattcggga aactcgagaa
      721 caaagcttga aaacggccgc atgccagtgt ttcatacgcg cacgatcgag agcatcctgg
      781 agccggtggc acagcagatc tcccacctgg tgataatgca cgaggagggc gaggtggacg
      841 gcaaagccat tcctgacctc accgcgcccg tggccgccgt gcaggcggcc gtcagcaacc
      901 tcgtccgggt tggaaaagag actgttcaaa ccactgagga tcagattttg aagagagata
      961 tgccaccagc atttattaag gttgagaatg cttgcaccaa gcttgtccag gcagctcaga
     1021 tgcttcagtc agacccttac tcagtgcctg ctcgagatta tctaattgat gggtcaaggg
     1081 gcatcctctc tggaacatca gacctgctcc ttaccttcga tgaggctgag gtccgtaaaa
     1141 ttattagagt ttgcaaagga attttggaat atcttacagt ggcagaggtg gtggagacta
     1201 tggaagattt ggtcacttac acaaagaatc ttgggccagg aatgactaag atggccaaga
     1261 tgattgacga gagacagcag gagctcactc accaggagca ccgagtgatg ttggtgaact
     1321 cgatgaacac cgtgaaagag ttgctgccag ttctcatttc agctatgaag atttttgtaa
     1381 caactaaaaa ctcaaaaaac caaggcatag aggaagcttt aaaaaatcgc aattttactg
     1441 tagaaaaaat gagtgctgaa attaatgaga taattcgtgt gttacaactc acctcttggg
     1501 atgaagatgc ctgggccagc aaggacactg aagccatgaa gagagcattg gcctccatag
     1561 actccaaact gaaccaggcc aaaggttggc tccgtgaccc tagtgcctcc ccaggggatg
     1621 ctggtgagca ggccatcaga cagatcttag atgaagctgg aaaagttggt gaactctgtg
     1681 caggcaaaga acgcagggag attctgggaa cttgcaaaat gctagggcag atgactgatc
     1741 aagtggctga cctccgtgcc agaggacaag gatcctcacc ggtggccatg cagaaagctc
     1801 agcaggtatc tcagggtctg gatgtgctca cagcaaaagt ggaaaatgca gctcgcaagc
     1861 tggaagccat gaccaactca aagcagagca ttgcaaagaa gatcgatgct gctcagaact
     1921 ggcttgcaga tccaaatggt ggaccggaag gagaagagca gattcgaggt gctttggctg
     1981 aagctcggaa aatagcagaa ttatgtgatg atcctaaaga aagagatgac attctacgtt
     2041 cccttgggga aatatctgct ctgacttcta aattagcaga tctacgaaga caggggaaag
     2101 gagattctcc agaggctcga gccttggcca aacaggtggc cacggccctg cagaacctgc
     2161 agaccaaaac caaccgggct gtggccaaca gcagaccggc caaagcagct gtacaccttg
     2221 agggcaagat tgagcaagca cagcggtgga ttgataatcc cacagtggat gaccgtggag
     2281 tcggtcaggc tgccatccgg gggcttgtgg ccgaagggca tcgtctggct aatgttatga
     2341 tggggcctta tcggcaagat cttctcgcca agtgtgaccg agtggaccag ctgacagccc
     2401 agctggctga cctggctgcc agaggggaag gggagagtcc tcaggcacga gcacttgcat
     2461 ctcagctcca agactcctta aaggatctaa aagctcggat gcaggaggcc atgactcagg
     2521 aagtgtcaga tgttttcagc gataccacaa ctcccatcaa gctgttggca gtggcagcca
     2581 cggcgcctcc tgatgcgcct aacagggaag aggtatttga tgagagggca gctaactttg
     2641 aaaaccattc aggaaagctt ggtgctacgg ccgagaaggc ggctgcggtt ggtactgcta
     2701 ataaatcaac agtggaaggc attcaggcct cagtgaagac ggcccgagaa ctcacacccc
     2761 aggtggtctc ggctgctcgt atcttactta ggaaccctgg aaatcaagct gcttatgaac
     2821 attttgagac catgaagaac cagtggatcg ataatgttga aaaaatgaca gggctggtgg
     2881 acgaagccat tgataccaaa tctctgttgg atgcttcaga agaagcaatt aaaaaagacc
     2941 tggacaagtg caaggtagct atggccaaca ttcagcctca gatgctggtt gctggggcaa
     3001 ccagtattgc tcgtcgggcc aaccggatcc tgctggtggc taagagggag gtggagaatt
     3061 ccgaggatcc caagttccgt gaggctgtga aagctgcctc tgatgaattg agcaaaacca
     3121 tctccccgat ggtgatggat gcaaaagctg tggctggaaa catttccgac cctggactgc
     3181 aaaagagctt cctggactca ggatatcgga tcctgggagc tgtggccaag gtcagagaag
     3241 ccttccaacc tcaggagcct gacttcccgc cgcctccacc agaccttgaa caactccgac
     3301 taacagatga gcttgctcct cccaaaccac ctctgcctga aggtgaggtc cctccaccta
     3361 ggcctccacc accagaggaa aaggatgaag agttccctga gcagaaggcc ggggaggtga
     3421 ttaaccagcc aatgatgatg gctgccagac agctccatga tgaagctcgc aaatggtcca
     3481 gcaagggcaa tgacatcatt gcagcagcca agcgcatggc tctgctgatg gctgagatgt
     3541 ctcggctggt aagagggggc agtggtacca agcgggcact cattcagtgt gccaaggaca
     3601 tcgccaaggc ctcagatgag gtgactcggt tggccaagga ggttgccaag cagtgcacag
     3661 ataaacggat tagaaccaac ctcttacagg tatgtgagcg aatcccaacc ataagcaccc
     3721 agctcaaaat cctgtccaca gtgaaggcca ccatgctggg ccggaccaac atcagtgatg
     3781 aggagtctga gcaggccaca gagatgctgg ttcacaatgc ccagaacctc atgcagtctg
     3841 tgaaggagac tgtgcgggaa gctgaagctg cttcaatcaa aattcgaaca gatgctggat
     3901 ttacactgcg ctgggttaga aagactccct ggtaccagta ggcggccgcg actctagatc
     3961 ataatcagcc ataccacat
//
