LOCUS       sequence-88716-e        1588 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 505 / 569, Emission: 516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1588)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 1588)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdEos-Actin-3
FEATURES             Location/Qualifiers
     source          1..1588
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      20..29
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             22..699
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="MVAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     CDS             745..1419
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="SAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVKE
                     GGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIARN
                     DITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLLE
                     GNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPDN
                     ARR"
ORIGIN
        1 gacgtcagat ccgctagcac catggtggcg attaagccag acatgaagat caacctccgt
       61 atggaaggca acgtaaacgg gcaccacttt gtgatcgacg gagatggtac aggcaagcct
      121 tttgagggaa aacagagtat ggatcttgaa gtcaaagagg gcggacctct gccttttgcc
      181 tttgatatcc tgaccactgc attccattac ggcaacaggg tattcgccga atatccagac
      241 cacatacaag actattttaa gcagtcgttt cctaaggggt attcgtggga acgaagcttg
      301 actttcgaag acgggggcat ttgcattgcc agaaacgaca taacaatgga aggggacact
      361 ttctataata aagttcgatt tcacggtgta aactttcccg ccaatggtcc agttatgcag
      421 aagaagacgc tgaaatggga gccctccact gagaaaatgt atgtgcgtga tggagtgctg
      481 accggtgata ttcgcatggc tttgttgctt gaaggaaatg cccattaccg atgtgacttc
      541 agaactactt acaaagctaa ggagaagggt gtcaagttac caggctacca ctttgtggac
      601 cactgcattg agattttaag ccatgacaaa gattacaaca aggttaagct gtatgagcat
      661 gctgttgctc attctggatt gcctgacaat gccagacgat ctagaggtca cggtactggt
      721 tctactggtt ctggttcttc tgagagtgcg attaagccag acatgaagat caacctccgt
      781 atggaaggca acgtaaacgg gcaccacttt gtgatcgacg gagatggtac aggcaagcct
      841 tttgagggaa aacagagtat ggatcttgaa gtcaaagagg gcggacctct gccttttgcc
      901 tttgatatcc tgaccactgc attccattac ggcaacaggg tattcgccga atatccagac
      961 cacatacaag actattttaa gcagtcgttt cctaaggggt attcgtggga acgaagcttg
     1021 actttcgaag acgggggcat ttgcattgcc agaaacgaca taacaatgga aggggacact
     1081 ttctataata aagttcgatt tcacggtgta aactttcccg ccaatggtcc agttatgcag
     1141 aagaagacgc tgaaatggga gccctccact gagaaaatgt atgtgcgtga tggagtgctg
     1201 accggtgata ttcgcatggc tttgttgctt gaaggaaatg cccattaccg atgtgacttc
     1261 agaactactt acaaagctaa ggagaagggt gtcaagttac caggctacca ctttgtggac
     1321 cactgcattg agattttaag ccatgacaaa gattacaaca aggttaagct gtatgagcat
     1381 gctgttgctc attctggatt gcctgacaat gccagacgat ctcgagctat ggatgatgat
     1441 atcgccgcgc tcgtcgtcga caacggctcc ggcatgtgca aggccggctt cgcgggcgac
     1501 gatgcccccc gggccgtctt cccctccatc gtggggcgcc ccaggcacca gggcgtgatg
     1561 gtgggcatgg gtcagaagga ttcctatg
//
