LOCUS       sequence-88718-e        2627 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 505 / 569, Emission: 516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Betzig E, Patterson GH, Sougrat R, Lindwasser OW, Olenych S, 
            Bonifacino JS, Davidson MW, Lippincott-Schwartz J, Hess HF
  TITLE     Imaging intracellular fluorescent proteins at nanometer resolution.
  JOURNAL   Science. 2006 Sep 15;313(5793):1642-5. Epub 2006 Aug 10.
  PUBMED    16902090
REFERENCE   2  (bases 1 to 2627)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2006-09-15"; volume: "313(5793)"; pages: "1642-5. Epub 2006 
            Aug 10"
COMMENT     Sequence Label: tdEos-Actin-7
FEATURES             Location/Qualifiers
     source          1..2627
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             33..710
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="MSAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     CDS             759..1433
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="SAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVKE
                     GGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIARN
                     DITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLLE
                     GNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPDN
                     ARR"
ORIGIN
        1 cgtcagatcc gctagcgcta ccggtcgcca ccatgagtgc gattaagcca gacatgaaga
       61 tcaacctccg tatggaaggc aacgtaaacg ggcaccactt tgtgatcgac ggagatggta
      121 caggcaagcc ttttgaggga aaacagagta tggatcttga agtcaaagag ggcggacctc
      181 tgccttttgc ctttgatatc ctgaccactg cattccatta cggcaacagg gtattcgccg
      241 aatatccaga ccacatacaa gactatttta agcagtcgtt tcctaagggg tattcgtggg
      301 aacgaagctt gactttcgaa gacgggggca tttgcattgc cagaaacgac ataacaatgg
      361 aaggggacac tttctataat aaagttcgat ttcacggtgt aaactttccc gccaatggtc
      421 cagttatgca gaagaagacg ctgaaatggg agccctccac tgagaaaatg tatgtgcgtg
      481 atggagtgct gactggtgat attcgcatgg ctttgttgct tgaaggaaat gcccattacc
      541 gatgtgactt cagaactact tacaaagcta aggagaaggg tgtcaagtta ccaggctacc
      601 actttgtgga ccactgcatt gagattttaa gccatgacaa agattacaac aaggttaagc
      661 tgtatgagca tgctgttgct cattctggat tgcctgacaa tgccagacga tccggtagcg
      721 gtcacggtac tggttctact ggttctggtt cttctgagag tgcgattaag ccagacatga
      781 agatcaacct ccgtatggaa ggcaacgtaa acgggcacca ctttgtgatc gacggagatg
      841 gtacaggcaa gccttttgag ggaaaacaga gtatggatct tgaagtcaaa gagggcggac
      901 ctctgccttt tgcctttgat atcctgacca ctgcattcca ttacggcaac agggtattcg
      961 ccgaatatcc agaccacata caagactatt ttaagcagtc gtttcctaag gggtattcgt
     1021 gggaacgaag cttgactttc gaagacgggg gcatttgcat tgccagaaac gacataacaa
     1081 tggaagggga cactttctat aataaagttc gatttcacgg tgtaaacttt cccgccaatg
     1141 gtccagttat gcagaagaag acgctgaaat gggagccctc cactgagaaa atgtatgtgc
     1201 gtgatggagt gctgactggt gatattcgca tggctttgtt gcttgaagga aatgcccatt
     1261 accgatgtga cttcagaact acttacaaag ctaaggagaa gggtgtcaag ttaccaggct
     1321 accactttgt ggaccactgc attgagattt taagccatga caaagattac aacaaggtta
     1381 agctgtatga gcatgctgtt gctcattctg gattgcctga caatgccaga cgatccggac
     1441 tcagatctcg agctatggat gatgatatcg ccgcgctcgt cgtcgacaac ggctccggca
     1501 tgtgcaaggc cggcttcgcg ggcgacgatg ccccccgggc cgtcttcccc tccatcgtgg
     1561 ggcgccccag gcaccagggc gtgatggtgg gcatgggtca gaaggattcc tatgtgggcg
     1621 acgaggccca gagcaagaga ggcatcctca ccctgaagta ccccatcgag cacggcatcg
     1681 tcaccaactg ggacgacatg gagaaaatct ggcaccacac cttctacaat gagctgcgtg
     1741 tggctcccga ggagcacccc gtgctgctga ccgaggcccc cctgaacccc aaggccaacc
     1801 gcgagaagat gacccagatc atgtttgaga ccttcaacac cccagccatg tacgttgcta
     1861 tccaggctgt gctatccctg tacgcctctg gccgtaccac tggcatcgtg atggactccg
     1921 gtgacggggt cacccacact gtgcccatct acgaggggta tgccctcccc catgccatcc
     1981 tgcgtctgga cctggctggc cgggacctga ctgactacct catgaagatc ctcaccgagc
     2041 gcggctacag cttcaccacc acggccgagc gggaaatcgt gcgtgacatt aaggagaagc
     2101 tgtgctacgt cgccctggac ttcgagcaag agatggccac ggctgcttcc agctcctccc
     2161 tggagaagag ctacgagctg cctgacggcc aggtcatcac cattggcaat gagcggttcc
     2221 gctgccctga ggcactcttc cagccttcct tcctgggcat ggagtcctgt ggcatccacg
     2281 aaactacctt caactccatc atgaagtgtg acgtggacat ccgcaaagac ctgtacgcca
     2341 acacagtgct gtctggcggc accaccatgt accctggcat tgccgacagg atgcagaagg
     2401 agatcactgc cctggcaccc agcacaatga agatcaagat cattgctcct cctgagcgca
     2461 agtactccgt gtggatcggc ggctccatcc tggcctcgct gtccaccttc cagcagatgt
     2521 ggatcagcaa gcaggagtat gacgagtccg gcccctccat cgtccaccgc aaatgcttct
     2581 agggatccac cggatctaga taactgatca taatcagcca taccaca
//
