LOCUS       sequence-88720-e        2620 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 505 / 569, Emission: 516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2620)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 2620)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdEos-Actin-N-10
FEATURES             Location/Qualifiers
     source          1..2620
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      15..24
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1176..1853
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="MSAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     CDS             1902..2579
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="SAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVKE
                     GGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIARN
                     DITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLLE
                     GNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPDN
                     ARR"
ORIGIN
        1 tccggctagc ggacgccacc atggatgatg atatcgccgc gctcgtcgtc gacaacggct
       61 ccggcatgtg caaggccggc ttcgcgggcg acgatgcccc ccgggccgtc ttcccctcca
      121 tcgtggggcg ccccaggcac cagggcgtga tggtgggcat gggtcagaag gattcctatg
      181 tgggcgacga ggcccagagc aagagaggca tcctcaccct gaagtacccc atcgagcacg
      241 gcatcgtcac caactgggac gacatggaga aaatctggca ccacaccttc tacaatgagc
      301 tgcgtgtggc tcccgaggag caccccgtgc tgctgaccga ggcccccctg aaccccaagg
      361 ccaaccgcga gaagatgacc cagatcatgt ttgagacctt caacacccca gccatgtacg
      421 ttgctatcca ggctgtgcta tccctgtacg cctctggccg taccactggc atcgtgatgg
      481 actccggtga cggggtcacc cacactgtgc ccatctacga ggggtatgcc ctcccccatg
      541 ccatcctgcg tctggacctg gctggccggg acctgactga ctacctcatg aagatcctca
      601 ccgagcgcgg ctacagcttc accaccacgg ccgagcggga aatcgtgcgt gacattaagg
      661 agaagctgtg ctacgtcgcc ctggacttcg agcaagagat ggccacggct gcttccagct
      721 cctccctgga gaagagctac gagctgcctg acggccaggt catcaccatt ggcaatgagc
      781 ggttccgctg ccctgaggca ctcttccagc cttccttcct gggcatggag tcctgtggca
      841 tccacgaaac taccttcaac tccatcatga agtgtgacgt ggacatccgc aaagacctgt
      901 acgccaacac agtgctgtct ggcggcacca ccatgtaccc tggcattgcc gacaggatgc
      961 agaaggagat cactgccctg gcacccagca caatgaagat caagatcatt gctcctcctg
     1021 agcgcaagta ctccgtgtgg atcggcggct ccatcctggc ctcgctgtcc accttccagc
     1081 agatgtggat cagcaagcag gagtatgacg agtccggccc ctccatcgtc caccgcaaat
     1141 gcttcagctc cggaggggat ccaccggtcg ccaccatgag tgcgattaag ccagacatga
     1201 agatcaacct ccgtatggaa ggcaacgtaa acgggcacca ctttgtgatc gacggagatg
     1261 gtacaggcaa gccttttgag ggaaaacaga gtatggatct tgaagtcaaa gagggcggac
     1321 ctctgccttt tgcctttgat atcctgacca ctgcattcca ttacggcaac agggtattcg
     1381 ccgaatatcc agaccacata caagactatt ttaagcagtc gtttcctaag gggtattcgt
     1441 gggaacgaag cttgactttc gaagacgggg gcatttgcat tgccagaaac gacataacaa
     1501 tggaagggga cactttctat aataaagttc gatttcacgg tgtaaacttt cccgccaatg
     1561 gtccagttat gcagaagaag acgctgaaat gggagccctc cactgagaaa atgtatgtgc
     1621 gtgatggagt gctgactggt gatattcgca tggctttgtt gcttgaagga aatgcccatt
     1681 accgatgtga cttcagaact acttacaaag ctaaggagaa gggtgtcaag ttaccaggct
     1741 accactttgt ggaccactgc attgagattt taagccatga caaagattac aacaaggtta
     1801 agctgtatga gcatgctgtt gctcattctg gattgcctga caatgccaga cgatccggta
     1861 gcggtcacgg tactggttct actggttctg gttcttctga gagtgcgatt aagccagaca
     1921 tgaagatcaa cctccgtatg gaaggcaacg taaacgggca ccactttgtg atcgacggag
     1981 atggtacagg caagcctttt gagggaaaac agagtatgga tcttgaagtc aaagagggcg
     2041 gacctctgcc ttttgccttt gatatcctga ccactgcatt ccattacggc aacagggtat
     2101 tcgccgaata tccagaccac atacaagact attttaagca gtcgtttcct aaggggtatt
     2161 cgtgggaacg aagcttgact ttcgaagacg ggggcatttg cattgccaga aacgacataa
     2221 caatggaagg ggacactttc tataataaag ttcgatttca cggtgtaaac tttcccgcca
     2281 atggtccagt tatgcagaag aagacgctga aatgggagcc ctccactgag aaaatgtatg
     2341 tgcgtgatgg agtgctgact ggtgatattc gcatggcttt gttgcttgaa ggaaatgccc
     2401 attaccgatg tgacttcaga actacttaca aagctaagga gaagggtgtc aagttaccag
     2461 gctaccactt tgtggaccac tgcattgaga ttttaagcca tgacaaagat tacaacaagg
     2521 ttaagctgta tgagcatgct gttgctcatt ctggattgcc tgacaatgcc agacgataag
     2581 cggccgcgac tctagatcat aatcagccat accaacattt
//
