LOCUS       sequence-88724-e        4214 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Alpha Actinin, Excitation: 505 / 569, Emission: 516 / 
            581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4214)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 4214)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdEos-Alpha-Actinin-19
FEATURES             Location/Qualifiers
     source          1..4214
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             2772..3449
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="MSAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     CDS             3498..4175
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="SAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVKE
                     GGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIARN
                     DITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLLE
                     GNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPDN
                     ARR"
ORIGIN
        1 cagatccgct agcgctaccg gactcagatc tcgagctcaa gcttcgcacc atcatggacc
       61 attatgattc tcagcaaacc aacgattaca tgcagccaga agaggactgg gaccgggacc
      121 tgctcctgga cccggcctgg gagaagcagc agagaaagac attcacggca tggtgtaact
      181 cccacctccg gaaggcgggg acacagatcg agaacatcga agaggacttc cgggatggcc
      241 tgaagctcat gctgctgctg gaggtcatct caggtgaacg cttggccaag ccagagcgag
      301 gcaagatgag agtgcacaag atctccaacg tcaacaaggc cctggatttc atagccagca
      361 aaggcgtcaa actggtgtcc atcggagccg aagaaatcgt ggatgggaat gtgaagatga
      421 ccctgggcat gatctggacc atcatcctgc gctttgccat ccaggacatc tccgtggaag
      481 agacttcagc caaggaaggg ctgctcctgt ggtgtcagag aaagacagcc ccttacaaaa
      541 atgtcaacat ccagaacttc cacataagct ggaaggatgg cctcggcttc tgtgctttga
      601 tccaccgaca ccggcccgag ctgattgact acgggaagct gcggaaggat gatccactca
      661 caaatctgaa tacggctttt gacgtggcag agaagtacct ggacatcccc aagatgctgg
      721 atgccgaaga catcgttgga actgcccgac cggatgagaa agccatcatg acttacgtgt
      781 ctagcttcta ccacgccttc tctggagccc agaaggcgga gacagcagcc aatcgcatct
      841 gcaaggtgtt ggccgtcaac caggagaacg agcagcttat ggaagactac gagaagctgg
      901 ccagtgatct gttggagtgg atccgccgca caatcccgtg gctggagaac cgggtgcccg
      961 agaacaccat gcatgccatg caacagaagc tggaggactt ccgggactac cggcgcctgc
     1021 acaagccgcc caaggtgcag gagaagtgcc agctggagat caacttcaac acgctgcaga
     1081 ccaagctgcg gctcagcaac cggcctgcct tcatgccctc tgagggcagg atggtctcgg
     1141 acatcaacaa tgcctggggc tgcctggagc aggtggagaa gggctatgag gagtggttgc
     1201 tgaatgagat ccggaggctg gagcgactgg accacctggc agagaagttc cggcagaagg
     1261 cctccatcca cgaggcctgg actgacggca aagaggccat gctgcgacag aaggactatg
     1321 agaccgccac cctctcggag atcaaggcct tgctcaagaa gcatgaggcc ttcgagagtg
     1381 acctggctgc ccaccaggac cgtgtggagc agattgccgc catcgcacag gagctcaatg
     1441 agctggacta ttatgactca cccagtgtca acgcccgttg ccaaaagatc tgtgaccagt
     1501 gggacaatct gggggcccta actcagaagc gaagggaagc tctggagcgg accgagaaac
     1561 tgctggagac cattgaccag ctgtacttgg agtatgccaa gcgggctgca cccttcaaca
     1621 actggatgga gggggccatg gaggacctgc aggacacctt cattgtgcac accattgagg
     1681 agatccaggg actgaccaca gcccatgagc agttcaaggc caccctccct gatgccgaca
     1741 aggagcgcct ggccatcctg ggcatccaca atgaggtgtc caagattgtc cagacctacc
     1801 acgtcaatat ggcgggcacc aacccctaca caaccatcac gcctcaggag atcaatggca
     1861 aatgggacca cgtgcggcag ctggtgcctc ggagggacca agctctgacg gaggagcatg
     1921 cccgacagca gcacaatgag aggctacgca agcagtttgg agcccaggcc aatgtcatcg
     1981 ggccctggat ccagaccaag atggaggaga tcgggaggat ctccattgag atgcatggga
     2041 ccctggagga ccagctcagc cacctgcggc agtatgagaa gagcatcgtc aactacaagc
     2101 caaagattga tcagctggag ggcgaccacc agctcatcca ggaggcgctc atcttcgaca
     2161 acaagcacac caactacacc atggagcaca tccgtgtggg ctgggagcag ctgctcacca
     2221 ccatcgccag gaccatcaat gaggtagaga accagatcct gacccgggat gccaagggca
     2281 tcagccagga gcagatgaat gagttccggg cctccttcaa ccactttgac cggaagaaga
     2341 caggcatgat ggacacggat gatttccgcg cctgcctgat ctccatgggt tacaacatgg
     2401 gagaagcaga atttgcccgc atcatgagca ttgtggaccc caaccgcctg ggggtagtga
     2461 cattccaggc cttcattgac ttcatgtccc gcgagacagc cgacacagat acagcagacc
     2521 aagtcatggc ttccttcaag atcctggctg gggacaagaa ctacattacc atggacgagc
     2581 tgcgccgcga gctgccaccc gaccaggctg agtactgcat cgcgcggatg gccccctaca
     2641 ccggccccga ctccgtgcca ggtgctctgg actacatgtc cttctccacg gcgctgtacg
     2701 gcgagagtga cctcaagctt cgaattctgc agtcgacggt accgcgggcc cgggatccac
     2761 cggtcgccac catgagtgcg attaagccag acatgaagat caacctccgt atggaaggca
     2821 acgtaaacgg gcaccacttt gtgatcgacg gagatggtac aggcaagcct tttgagggaa
     2881 aacagagtat ggatcttgaa gtcaaagagg gcggacctct gccttttgcc tttgatatcc
     2941 tgaccactgc attccattac ggcaacaggg tattcgccga atatccagac cacatacaag
     3001 actattttaa gcagtcgttt cctaaggggt attcgtggga acgaagcttg actttcgaag
     3061 acgggggcat ttgcattgcc agaaacgaca taacaatgga aggggacact ttctataata
     3121 aagttcgatt tcacggtgta aactttcccg ccaatggtcc agttatgcag aagaagacgc
     3181 tgaaatggga gccctccact gagaaaatgt atgtgcgtga tggagtgctg actggtgata
     3241 ttcgcatggc tttgttgctt gaaggaaatg cccattaccg atgtgacttc agaactactt
     3301 acaaagctaa ggagaagggt gtcaagttac caggctacca ctttgtggac cactgcattg
     3361 agattttaag ccatgacaaa gattacaaca aggttaagct gtatgagcat gctgttgctc
     3421 attctggatt gcctgacaat gccagacgat ccggtagcgg tcacggtact ggttctactg
     3481 gttctggttc ttctgagagt gcgattaagc cagacatgaa gatcaacctc cgtatggaag
     3541 gcaacgtaaa cgggcaccac tttgtgatcg acggagatgg tacaggcaag ccttttgagg
     3601 gaaaacagag tatggatctt gaagtcaaag agggcggacc tctgcctttt gcctttgata
     3661 tcctgaccac tgcattccat tacggcaaca gggtattcgc cgaatatcca gaccacatac
     3721 aagactattt taagcagtcg tttcctaagg ggtattcgtg ggaacgaagc ttgactttcg
     3781 aagacggggg catttgcatt gccagaaacg acataacaat ggaaggggac actttctata
     3841 ataaagttcg atttcacggt gtaaactttc ccgccaatgg tccagttatg cagaagaaga
     3901 cgctgaaatg ggagccctcc actgagaaaa tgtatgtgcg tgatggagtg ctgactggtg
     3961 atattcgcat ggctttgttg cttgaaggaa atgcccatta ccgatgtgac ttcagaacta
     4021 cttacaaagc taaggagaag ggtgtcaagt taccaggcta ccactttgtg gaccactgca
     4081 ttgagatttt aagccatgac aaagattaca acaaggttaa gctgtatgag catgctgttg
     4141 ctcattctgg attgcctgac aatgccagac gataagcggc cgcgactcta gatcataatc
     4201 agccatacca catt
//
