LOCUS       sequence-88725-e        4247 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Alpha Actinin, Excitation: 505 / 569, Emission: 516 / 
            581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4247)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 4247)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdEos-Alpha-Actinin2-N-18
FEATURES             Location/Qualifiers
     source          1..4247
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             2808..3485
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="MSAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     CDS             3534..4211
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="SAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVKE
                     GGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIARN
                     DITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLLE
                     GNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPDN
                     ARR"
ORIGIN
        1 gatccgctag cgctaccgga ctcagatctc gagaccgcct agcgctaccg gactcagatc
       61 tcgagaccgc catgaaccag atagagcccg gcgtgcagta caactacgtg tacgacgagg
      121 atgagtacat gatccaggag gaggagtggg accgcgacct gctcctggac ccagcctggg
      181 agaagcagca gaggaagacc ttcactgcct ggtgtaactc ccacctaagg aaagccggca
      241 cccagattga gaacatcgag gaagacttca ggaatggcct taagctcatg ctgcttttgg
      301 aagtcatctc aggggaaagg ctgcccaaac ctgaccgggg aaaaatgcgg ttccacaaaa
      361 ttgctaatgt caacaaagct ttggattaca tagccagcaa aggggtgaaa ctggtgtcca
      421 tcggcgctga agaaattgtt gatggcaatg tgaaaatgac cctgggtatg atctggacca
      481 tcatccttcg ctttgctatt caggatattt cggttgaaga aacatctgcc aaagaaggtc
      541 tgctgctttg gtgtcagagg aaaactgctc cttatagaaa tgtgaacatt cagaacttcc
      601 atactagctg gaaagatggc cttggactct gtgccctcat ccaccgacac cggcctgacc
      661 tcattgacta ctcaaagctt aacaaggatg accccatagg aaatattaac ctggccatgg
      721 aaatcgctga gaagcacctg gatattccta aaatgttgga tgctgaagac atcgtgaaca
      781 cccctaaacc cgatgaaaga gccatcatga cgtacgtctc ttgcttctac cacgcttttg
      841 cgggcgcgga gcaggccgag acagcggcta acaggatatg taaggttctt gctgtgaatc
      901 aagagaatga gaggctgatg gaagaatatg agaggctagc gagtgagctt ttggaatgga
      961 ttcgtcgcac gatcccctgg ctggagaacc ggactcccga gaagaccatg caagccatgc
     1021 agaagaagct ggaggacttc cgggattacc gccggaagca caagccaccc aaggtgcagg
     1081 agaaatgcca gctggagatc aacttcaaca cgctgcagac caagctgcgg atcagcaacc
     1141 gtcctgcctt catgccctcc gagggcaaga tggtgtcgga tattgctggt gcctggcaga
     1201 ggctggagca ggctgagaag ggttacgagg agtggttgct caatgagatt cggagactgg
     1261 agcgcttgga acacctggct gagaagttca ggcagaaggc ctcaacgcac gagacttggg
     1321 cttatggcaa agagcagatc ttgctgcaga aggattacga gtcggcgtcg ctgacagagg
     1381 tgcgggctct gctgcggaag cacgaggcgt tcgagagcga cctggcagcg caccaggacc
     1441 gcgtggagca gatcgcagcc atcgcgcagg agctcaatga actggactat cacgacgctg
     1501 tgaatgtcaa tgatcggtgc cagaaaattt gtgaccagtg ggaccgactg ggaacgctta
     1561 ctcagaagag gagagaagcc ctagagagaa tggagaaatt gctagaaacc attgatcagc
     1621 ttcacctgga gtttgccaag agggctgctc ctttcaacaa ttggatggag ggcgctatgg
     1681 aggatctgca agatatgttc attgtccaca gcattgagga gatccagagt ctgatcactg
     1741 cgcatgagca gttcaaggcc acgctgcccg aggcggacgg agagcggcag tccatcatgg
     1801 ccatccagaa cgaggtggag aaggtgattc agagctacaa catcagaatc agctcaagca
     1861 acccgtacag cactgtcacc atggatgagc tccggaccaa gtgggacaag gtgaagcaac
     1921 tcgtgcccat ccgcgatcaa tccctgcagg aggagctggc tcgccagcat gctaacgagc
     1981 gtctgaggcg ccagtttgct gcccaagcca atgccattgg gccctggatc cagaacaaga
     2041 tggaggagat tgcccggagc tccatccaga tcacaggagc cctggaagac cagatgaacc
     2101 agctgaagca gtatgagcac aacatcatca actataagaa caacatcgac aagctggagg
     2161 gagaccatca gctcatccag gaggcccttg tctttgacaa caagcacacg aactacacga
     2221 tggagcacat tcgtgttgga tgggagctgc tgctgacaac catcgccaga accatcaatg
     2281 aggtggagac tcagatcctg acgagagatg cgaagggcat cacccaggag cagatgaatg
     2341 agttcagagc ctccttcaac cactttgaca ggaggaagaa tggcctgatg gatcatgagg
     2401 atttcagagc ctgcctgatt tccatgggtt atgacctggg tgaagccgaa tttgcccgca
     2461 ttatgaccct ggtagatccc aacgggcaag gcaccgtcac cttccaatcc ttcatcgact
     2521 tcatgactag agagacggct gacaccgaca ctgccgagca ggtcatcgcc tccttccgga
     2581 tcctggcttc tgataagcca tacatcctgg cggaggagct gcgtcgggag ctgcccccgg
     2641 atcaggccca gtactgcatc aagaggatgc ccgcctactc gggcccaggc agtgtgcctg
     2701 gtgcactgga ttacgctgcg ttctcttccg cactctacgg ggagagcgat ctgggtagcg
     2761 catccggggg tgcgtcaggc ggcagcggat ctggaccggt cgccaccatg agtgcgatta
     2821 agccagacat gaagatcaac ctccgtatgg aaggcaacgt aaacgggcac cactttgtga
     2881 tcgacggaga tggtacaggc aagccttttg agggaaaaca gagtatggat cttgaagtca
     2941 aagagggcgg acctctgcct tttgcctttg atatcctgac cactgcattc cattacggca
     3001 acagggtatt cgccgaatat ccagaccaca tacaagacta ttttaagcag tcgtttccta
     3061 aggggtattc gtgggaacga agcttgactt tcgaagacgg gggcatttgc attgccagaa
     3121 acgacataac aatggaaggg gacactttct ataataaagt tcgatttcac ggtgtaaact
     3181 ttcccgccaa tggtccagtt atgcagaaga agacgctgaa atgggagccc tccactgaga
     3241 aaatgtatgt gcgtgatgga gtgctgactg gtgatattcg catggctttg ttgcttgaag
     3301 gaaatgccca ttaccgatgt gacttcagaa ctacttacaa agctaaggag aagggtgtca
     3361 agttaccagg ctaccacttt gtggaccact gcattgagat tttaagccat gacaaagatt
     3421 acaacaaggt taagctgtat gagcatgctg ttgctcattc tggattgcct gacaatgcca
     3481 gacgatccgg tagcggtcac ggtactggtt ctactggttc tggttcttct gagagtgcga
     3541 ttaagccaga catgaagatc aacctccgta tggaaggcaa cgtaaacggg caccactttg
     3601 tgatcgacgg agatggtaca ggcaagcctt ttgagggaaa acagagtatg gatcttgaag
     3661 tcaaagaggg cggacctctg ccttttgcct ttgatatcct gaccactgca ttccattacg
     3721 gcaacagggt attcgccgaa tatccagacc acatacaaga ctattttaag cagtcgtttc
     3781 ctaaggggta ttcgtgggaa cgaagcttga ctttcgaaga cgggggcatt tgcattgcca
     3841 gaaacgacat aacaatggaa ggggacactt tctataataa agttcgattt cacggtgtaa
     3901 actttcccgc caatggtcca gttatgcaga agaagacgct gaaatgggag ccctccactg
     3961 agaaaatgta tgtgcgtgat ggagtgctga ctggtgatat tcgcatggct ttgttgcttg
     4021 aaggaaatgc ccattaccga tgtgacttca gaactactta caaagctaag gagaagggtg
     4081 tcaagttacc aggctaccac tttgtggacc actgcattga gattttaagc catgacaaag
     4141 attacaacaa ggttaagctg tatgagcatg ctgttgctca ttctggattg cctgacaatg
     4201 ccagacgata agcggccgcg actctagatc ataatcagcc ataccac
//
