LOCUS       sequence-88726-e        4232 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Alpha Actinin, Excitation: 505 / 569, Emission: 516 / 
            581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4232)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 4232)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdEos-Alpha-Actinin-C-14
FEATURES             Location/Qualifiers
     source          1..4232
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             34..711
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="MSAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     CDS             760..1434
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="SAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVKE
                     GGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIARN
                     DITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLLE
                     GNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPDN
                     ARR"
ORIGIN
        1 acgtcagatc cgctagcgct accggtcgcc accatgagtg cgattaagcc agacatgaag
       61 atcaacctcc gtatggaagg caacgtaaac gggcaccact ttgtgatcga cggagatggt
      121 acaggcaagc cttttgaggg aaaacagagt atggatcttg aagtcaaaga gggcggacct
      181 ctgccttttg cctttgatat cctgaccact gcattccatt acggcaacag ggtattcgcc
      241 gaatatccag accacataca agactatttt aagcagtcgt ttcctaaggg gtattcgtgg
      301 gaacgaagct tgactttcga agacgggggc atttgcattg ccagaaacga cataacaatg
      361 gaaggggaca ctttctataa taaagttcga tttcacggtg taaactttcc cgccaatggt
      421 ccagttatgc agaagaagac gctgaaatgg gagccctcca ctgagaaaat gtatgtgcgt
      481 gatggagtgc tgactggtga tattcgcatg gctttgttgc ttgaaggaaa tgcccattac
      541 cgatgtgact tcagaactac ttacaaagct aaggagaagg gtgtcaagtt accaggctac
      601 cactttgtgg accactgcat tgagatttta agccatgaca aagattacaa caaggttaag
      661 ctgtatgagc atgctgttgc tcattctgga ttgcctgaca atgccagacg atccggtagc
      721 ggtcacggta ctggttctac tggttctggt tcttctgaga gtgcgattaa gccagacatg
      781 aagatcaacc tccgtatgga aggcaacgta aacgggcacc actttgtgat cgacggagat
      841 ggtacaggca agccttttga gggaaaacag agtatggatc ttgaagtcaa agagggcgga
      901 cctctgcctt ttgcctttga tatcctgacc actgcattcc attacggcaa cagggtattc
      961 gccgaatatc cagaccacat acaagactat tttaagcagt cgtttcctaa ggggtattcg
     1021 tgggaacgaa gcttgacttt cgaagacggg ggcatttgca ttgccagaaa cgacataaca
     1081 atggaagggg acactttcta taataaagtt cgatttcacg gtgtaaactt tcccgccaat
     1141 ggtccagtta tgcagaagaa gacgctgaaa tgggagccct ccactgagaa aatgtatgtg
     1201 cgtgatggag tgctgactgg tgatattcgc atggctttgt tgcttgaagg aaatgcccat
     1261 taccgatgtg acttcagaac tacttacaaa gctaaggaga agggtgtcaa gttaccaggc
     1321 taccactttg tggaccactg cattgagatt ttaagccatg acaaagatta caacaaggtt
     1381 aagctgtatg agcatgctgt tgctcattct ggattgcctg acaatgccag acgatccgga
     1441 ctcagatctc gaggaagcgg cggatctggg tccggagacc attatgattc tcagcaaacc
     1501 aacgattaca tgcagccaga agaggactgg gaccgggacc tgctcctgga cccggcctgg
     1561 gagaagcagc agagaaagac attcacggca tggtgtaact cccacctccg gaaggcgggg
     1621 acacagatcg agaacatcga agaggacttc cgggatggcc tgaagctcat gctgctgctg
     1681 gaggtcatct caggtgaacg cttggccaag ccagagcgag gcaagatgag agtgcacaag
     1741 atctccaacg tcaacaaggc cctggatttc atagccagca aaggcgtcaa actggtgtcc
     1801 atcggagccg aagaaatcgt ggatgggaat gtgaagatga ccctgggcat gatctggacc
     1861 atcatcctgc gctttgccat ccaggacatc tccgtggaag agacttcagc caaggaaggg
     1921 ctgctcctgt ggtgtcagag aaagacagcc ccttacaaaa atgtcaacat ccagaacttc
     1981 cacataagct ggaaggatgg cctcggcttc tgtgctttga tccaccgaca ccggcccgag
     2041 ctgattgact acgggaagct gcggaaggat gatccactca caaatctgaa tacggctttt
     2101 gacgtggcag agaagtacct ggacatcccc aagatgctgg atgccgaaga catcgttgga
     2161 actgcccgac cggatgagaa agccatcatg acttacgtgt ctagcttcta ccacgccttc
     2221 tctggagccc agaaggcgga gacagcagcc aatcgcatct gcaaggtgtt ggccgtcaac
     2281 caggagaacg agcagcttat ggaagactac gagaagctgg ccagtgatct gttggagtgg
     2341 atccgccgca caatcccgtg gctggagaac cgggtgcccg agaacaccat gcatgccatg
     2401 caacagaagc tggaggactt ccgggactac cggcgcctgc acaagccgcc caaggtgcag
     2461 gagaagtgcc agctggagat caacttcaac acgctgcaga ccaagctgcg gctcagcaac
     2521 cggcctgcct tcatgccctc tgagggcagg atggtctcgg acatcaacaa tgcctggggc
     2581 tgcctggagc aggtggagaa gggctatgag gagtggttgc tgaatgagat ccggaggctg
     2641 gagcgactgg accacctggc agagaagttc cggcagaagg cctccatcca cgaggcctgg
     2701 actgacggca aagaggccat gctgcgacag aaggactatg agaccgccac cctctcggag
     2761 atcaaggcct tgctcaagaa gcatgaggcc ttcgagagtg acctggctgc ccaccaggac
     2821 cgtgtggagc agattgccgc catcgcacag gagctcaatg agctggacta ttatgactca
     2881 cccagtgtca acgcccgttg ccaaaagatc tgtgaccagt gggacaatct gggggcccta
     2941 actcagaagc gaagggaagc tctggagcgg accgagaaac tgctggagac cattgaccag
     3001 ctgtacttgg agtatgccaa gcgggctgca cccttcaaca actggatgga gggggccatg
     3061 gaggacctgc aggacacctt cattgtgcac accattgagg agatccaggg actgaccaca
     3121 gcccatgagc agttcaaggc caccctccct gatgccgaca aggagcgcct ggccatcctg
     3181 ggcatccaca atgaggtgtc caagattgtc cagacctacc acgtcaatat ggcgggcacc
     3241 aacccctaca caaccatcac gcctcaggag atcaatggca aatgggacca cgtgcggcag
     3301 ctggtgcctc ggagggacca agctctgacg gaggagcatg cccgacagca gcacaatgag
     3361 aggctacgca agcagtttgg agcccaggcc aatgtcatcg ggccctggat ccagaccaag
     3421 atggaggaga tcgggaggat ctccattgag atgcatggga ccctggagga ccagctcagc
     3481 cacctgcggc agtatgagaa gagcatcgtc aactacaagc caaagattga tcagctggag
     3541 ggcgaccacc agctcatcca ggaggcgctc atcttcgaca acaagcacac caactacacc
     3601 atggagcaca tccgtgtggg ctgggagcag ctgctcacca ccatcgccag gaccatcaat
     3661 gaggtagaga accagatcct gacccgggat gccaagggca tcagccagga gcagatgaat
     3721 gagttccggg cctccttcaa ccactttgac cgggatcact ccggcacact gggtcccgag
     3781 gagttcaaag cctgcctcat cagcttgggt tatgatattg gcaacgaccc ccagggagaa
     3841 gcagaatttg cccgcatcat gagcattgtg gaccccaacc gcctgggggt agtgacattc
     3901 caggccttca ttgacttcat gtcccgcgag acagccgaca cagatacagc agaccaagtc
     3961 atggcttcct tcaagatcct ggctggggac aagaactaca ttaccatgga cgagctgcgc
     4021 cgcgagctgc cacccgacca ggctgagtac tgcatcgcgc ggatggcccc ctacaccggc
     4081 cccgactccg tgccaggtgc tctggactac atgtccttct ccacggcgct gtacggcgag
     4141 agtgacctct aagaattctg cagtcgacgg taccgcgggc ccgggatcca ccggatctag
     4201 ataactgatc ataatcagcc ataccacatt tg
//
