LOCUS       sequence-88732-e        2733 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin Related Protein, Excitation: 505 / 569, 
            Emission: 516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2733)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 2733)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdEos-ARP3-N-12
FEATURES             Location/Qualifiers
     source          1..2733
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      12..21
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1308..1985
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="MSAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     CDS             2034..2711
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="SAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVKE
                     GGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIARN
                     DITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLLE
                     GNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPDN
                     ARR"
ORIGIN
        1 atccgctagc agccaccatg gcgggacggc tgccggcctg tgtggtggac tgtggcacgg
       61 ggtatacaaa actaggatat gctggaaata cagaaccaca gtttatcatc ccttcctgta
      121 ttgctattaa ggagtcagca aaagtgggtg atcaagctca aaggagggtg atgaaaggtg
      181 ttgatgacct agacttcttc attggtgatg aagcaataga aaaacctaca tatgcaacaa
      241 agtggccaat ccgccatggt atagttgaag attgggactt aatggaaagg tttatggagc
      301 aagtgatctt taaatattta agggcagaac ctgaagacca ttattttctt ttgactgaac
      361 ctccattgaa tactccagaa aacagggaat atactgctga aataatgttt gagtccttca
      421 atgttccagg cttgtacatt gctgtgcagg ctgttcttgc cttagctgca tcttggacct
      481 caagacaagt aggagaacgg acgttgaccg gtacggtaat agacagtgga gatggtgtca
      541 ctcatgtcat tcctgtggct gaagggtatg tgattggcag ctgtattaaa cacattccaa
      601 tcgcaggacg agatataaca tattttattc agcaactgct gagagaccga gaagtaggaa
      661 tccctccaga acaatccttg gaaactgcta aggcagtaaa ggagcgctat agttatgtct
      721 gcccagattt agtaaaagaa tttaacaagt atgatacaga tgggtcaaaa tggattaaac
      781 agtatactgg aatcaatgct atctcaaaga aagagttttc tatcgatgtt ggttatgaga
      841 gatttttggg acctgaaatc ttttttcatc cagagtttgc taatccagac tttacacaac
      901 ctatctcaga agttgtagat gaagtaattc agaattgtcc tattgatgtc agacgtcctc
      961 tctacaagaa tattgtcctc tctggaggtt caaccatgtt cagggacttt ggacgtcgct
     1021 tgcaaagaga tttgaaaaga actgtagatg cccggctgaa attaagtgag gaattgagtg
     1081 gtggtagatt gaagccaaaa cctattgatg tacaagtcat tacacaccac atgcagcgat
     1141 atgcagtttg gtttggagga tcaatgctgg cttccacgcc tgagttctac caagtatgcc
     1201 acaccaaaaa ggattatgaa gaaattggac ctagcatttg tcgtcacaat ccagtgtttg
     1261 gagtcatgtc ggcatccggc ggtagcgggg atccaccggt cgccaccatg agtgcgatta
     1321 agccagacat gaagatcaac ctccgtatgg aaggcaacgt aaacgggcac cactttgtga
     1381 tcgacggaga tggtacaggc aagccttttg agggaaaaca gagtatggat cttgaagtca
     1441 aagagggcgg acctctgcct tttgcctttg atatcctgac cactgcattc cattacggca
     1501 acagggtatt cgccgaatat ccagaccaca tacaagacta ttttaagcag tcgtttccta
     1561 aggggtattc gtgggaacga agcttgactt tcgaagacgg gggcatttgc attgccagaa
     1621 acgacataac aatggaaggg gacactttct ataataaagt tcgatttcac ggtgtaaact
     1681 ttcccgccaa tggtccagtt atgcagaaga agacgctgaa atgggagccc tccactgaga
     1741 aaatgtatgt gcgtgatgga gtgctgactg gtgatattcg catggctttg ttgcttgaag
     1801 gaaatgccca ttaccgatgt gacttcagaa ctacttacaa agctaaggag aagggtgtca
     1861 agttaccagg ctaccacttt gtggaccact gcattgagat tttaagccat gacaaagatt
     1921 acaacaaggt taagctgtat gagcatgctg ttgctcattc tggattgcct gacaatgcca
     1981 gacgatccgg tagcggtcac ggtactggtt ctactggttc tggttcttct gagagtgcga
     2041 ttaagccaga catgaagatc aacctccgta tggaaggcaa cgtaaacggg caccactttg
     2101 tgatcgacgg agatggtaca ggcaagcctt ttgagggaaa acagagtatg gatcttgaag
     2161 tcaaagaggg cggacctctg ccttttgcct ttgatatcct gaccactgca ttccattacg
     2221 gcaacagggt attcgccgaa tatccagacc acatacaaga ctattttaag cagtcgtttc
     2281 ctaaggggta ttcgtgggaa cgaagcttga ctttcgaaga cgggggcatt tgcattgcca
     2341 gaaacgacat aacaatggaa ggggacactt tctataataa agttcgattt cacggtgtaa
     2401 actttcccgc caatggtcca gttatgcaga agaagacgct gaaatgggag ccctccactg
     2461 agaaaatgta tgtgcgtgat ggagtgctga ctggtgatat tcgcatggct ttgttgcttg
     2521 aaggaaatgc ccattaccga tgtgacttca gaactactta caaagctaag gagaagggtg
     2581 tcaagttacc aggctaccac tttgtggacc actgcattga gattttaagc catgacaaag
     2641 attacaacaa ggttaagctg tatgagcatg ctgttgctca ttctggattg cctgacaatg
     2701 ccagacgata agcggccgcg actctagatc ata
//
