LOCUS       sequence-88735-e        3894 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Tight Junctions, Excitation: 505 / 569, Emission: 516 
            / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3894)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 3894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdEos-Beta-Catenin-20
FEATURES             Location/Qualifiers
     source          1..3894
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             45..722
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="MSAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     CDS             771..1445
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="SAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVKE
                     GGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIARN
                     DITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLLE
                     GNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPDN
                     ARR"
     regulatory      1500..1509
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1551..1586
                     /codon_start=1
                     /product="nanobody epitope tag corresponding to amino acids
                     16-27 of human beta-catenin (Traenkle et al., 2015)"
                     /label=BC2 tag
                     /translation="PDRKAAVSHWQQ"
ORIGIN
        1 ggtttagtga accgtcagat ccgctagcgc taccggtcgc caccatgagt gcgattaagc
       61 cagacatgaa gatcaacctc cgtatggaag gcaacgtaaa cgggcaccac tttgtgatcg
      121 acggagatgg tacaggcaag ccttttgagg gaaaacagag tatggatctt gaagtcaaag
      181 agggcggacc tctgcctttt gcctttgata tcctgaccac tgcattccat tacggcaaca
      241 gggtattcgc cgaatatcca gaccacatac aagactattt taagcagtcg tttcctaagg
      301 ggtattcgtg ggaacgaagc ttgactttcg aagacggggg catttgcatt gccagaaacg
      361 acataacaat ggaaggggac actttctata ataaagttcg atttcacggt gtaaactttc
      421 ccgccaatgg tccagttatg cagaagaaga cgctgaaatg ggagccctcc actgagaaaa
      481 tgtatgtgcg tgatggagtg ctgactggtg atattcgcat ggctttgttg cttgaaggaa
      541 atgcccatta ccgatgtgac ttcagaacta cttacaaagc taaggagaag ggtgtcaagt
      601 taccaggcta ccactttgtg gaccactgca ttgagatttt aagccatgac aaagattaca
      661 acaaggttaa gctgtatgag catgctgttg ctcattctgg attgcctgac aatgccagac
      721 gatccggtag cggtcacggt actggttcta ctggttctgg ttcttctgag agtgcgatta
      781 agccagacat gaagatcaac ctccgtatgg aaggcaacgt aaacgggcac cactttgtga
      841 tcgacggaga tggtacaggc aagccttttg agggaaaaca gagtatggat cttgaagtca
      901 aagagggcgg acctctgcct tttgcctttg atatcctgac cactgcattc cattacggca
      961 acagggtatt cgccgaatat ccagaccaca tacaagacta ttttaagcag tcgtttccta
     1021 aggggtattc gtgggaacga agcttgactt tcgaagacgg gggcatttgc attgccagaa
     1081 acgacataac aatggaaggg gacactttct ataataaagt tcgatttcac ggtgtaaact
     1141 ttcccgccaa tggtccagtt atgcagaaga agacgctgaa atgggagccc tccactgaga
     1201 aaatgtatgt gcgtgatgga gtgctgactg gtgatattcg catggctttg ttgcttgaag
     1261 gaaatgccca ttaccgatgt gacttcagaa ctacttacaa agctaaggag aagggtgtca
     1321 agttaccagg ctaccacttt gtggaccact gcattgagat tttaagccat gacaaagatt
     1381 acaacaaggt taagctgtat gagcatgctg ttgctcattc tggattgcct gacaatgcca
     1441 gacgatccgg actcagatct cgagctcaag cttcgaattc tgcagtcgac ggtaccgcgg
     1501 ccaccatggc tactcaagct gacctgatgg agttggacat ggccatggag ccggacagaa
     1561 aagctgctgt cagccactgg cagcagcagt cttacttgga ttctggaatc cattctggtg
     1621 ccaccaccac agctccttcc ctgagtggca agggcaaccc tgaggaagaa gatgttgaca
     1681 cctcccaagt cctttatgaa tgggagcaag gcttttccca gtccttcacg caagagcaag
     1741 tagctgatat tgacgggcag tatgcaatga ctagggctca gagggtccga gctgccatgt
     1801 tccctgagac gctagatgag ggcatgcaga tcccatccac gcagtttgac gctgctcatc
     1861 ccactaatgt ccagcgcttg gctgaaccat cacagatgtt gaaacatgca gttgtcaatt
     1921 tgattaacta tcaggatgac gcggaacttg ccacacgtgc aattcctgag ctgacaaaac
     1981 tgctaaacga tgaggaccag gtggtagtta ataaagctgc tgttatggtc catcagcttt
     2041 ccaaaaagga agcttccaga catgccatca tgcgctcccc tcagatggtg tctgccattg
     2101 tacgcaccat gcagaataca aatgatgtag agacagctcg ttgtactgct gggactctgc
     2161 acaacctttc tcaccaccgc gagggcttgc tggccatctt taagtctggt ggcatcccag
     2221 cgctggtgaa aatgcttggg tcaccagtgg attctgtact gttctacgcc atcacgacac
     2281 tgcataatct cctgctccat caggaaggag ctaaaatggc agtgcgccta gctggtggac
     2341 tgcagaaaat ggttgctttg ctcaacaaaa caaacgtgaa attcttggct attacaacag
     2401 actgccttca gatcttagct tatggcaatc aagagagcaa gctcatcatt ctggccagtg
     2461 gtggacccca agccttagta aacataatga ggacctacac ttatgagaag cttctgtgga
     2521 ccacaagcag agtgctgaaa gtgctgtctg tctgctctag caacaagccg gccattgtag
     2581 aagctggtgg gatgcaggca ctggggcttc atctgacaga cccaagtcag cgacttgttc
     2641 aaaactgtct ttggactctc agaaaccttt cagatgcagc gactaagcag gaagggatgg
     2701 aaggcctcct tgggactcta gtgcagcttc tgggttccga tgatataaat gtggtcacct
     2761 gtgcagctgg aattctctct aacctcactt gcaataatta caaaaacaag atgatggtgt
     2821 gccaagtggg tggcatagag gctcttgtac gcaccgtcct tcgtgctggt gacagggaag
     2881 acatcactga gcctgccatc tgtgctcttc gtcatctgac cagccggcat caggaagccg
     2941 agatggccca gaatgccgtt cgccttcatt atggactgcc tgttgtggtt aaactcctgc
     3001 acccaccatc ccactggcct ctgataaagg caactgttgg attgattcga aaccttgccc
     3061 tttgcccagc aaatcatgcg cctttgcggg aacagggtgc tattccacga ctagttcagc
     3121 tgcttgtacg agcacatcag gacacccaac ggcgcacctc catgggtgga acgcagcagc
     3181 agtttgtgga gggcgtgcgc atggaggaga tagtagaagg gtgtactgga gctctccaca
     3241 tccttgctcg ggacgttcac aaccggattg taatccgagg actcaatacc attccattgt
     3301 ttgtgcagtt gctttattct cccattgaaa atatccaaag agtagctgca ggggtcctct
     3361 gtgaacttgc tcaggacaag gaggctgcag aggccattga agctgaggga gccacagctc
     3421 ccctgacaga gttactccac tccaggaatg aaggcgtggc aacatacgca gctgctgtcc
     3481 tattccgaat gtctgaggac aagccacagg attacaagaa gcggctttca gtcgagctga
     3541 ccagttccct cttcaggaca gagccaatgg cttggaatga gactgcagat cttggactgg
     3601 acattggtgc ccagggagaa gcccttggat atcgccagga tgatcccagc taccgttctt
     3661 ttcactctgg tggatacggc caggatgcct tggggatgga ccctatgatg gagcatgaga
     3721 tgggtggcca ccaccctggt gctgactatc cagttgatgg gctgcctgat ctgggacacg
     3781 cccaggacct catggatggg ctgcccccag gtgatagcaa tcagctggcc tggtttgata
     3841 ctgacctgta aaagcttgga tccaccggat ctagataact gatcataatc agcc
//
