LOCUS       sequence-88736-e        4249 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Chromatin Assembly Factor 1, Excitation: 505 / 569, 
            Emission: 516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4249)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 4249)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdEos-CAF1-C-10
FEATURES             Location/Qualifiers
     source          1..4249
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             34..711
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="MSAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     CDS             760..1434
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="SAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVKE
                     GGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIARN
                     DITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLLE
                     GNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPDN
                     ARR"
     protein_bind    3253..3264
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
ORIGIN
        1 gacgtcagat cgctagcgct accggtcgcc accatgagtg cgattaagcc agacatgaag
       61 atcaacctcc gtatggaagg caacgtaaac gggcaccact ttgtgatcga cggagatggt
      121 acaggcaagc cttttgaggg aaaacagagt atggatcttg aagtcaaaga gggcggacct
      181 ctgccttttg cctttgatat cctgaccact gcattccatt acggcaacag ggtattcgcc
      241 gaatatccag accacataca agactatttt aagcagtcgt ttcctaaggg gtattcgtgg
      301 gaacgaagct tgactttcga agacgggggc atttgcattg ccagaaacga cataacaatg
      361 gaaggggaca ctttctataa taaagttcga tttcacggtg taaactttcc cgccaatggt
      421 ccagttatgc agaagaagac gctgaaatgg gagccctcca ctgagaaaat gtatgtgcgt
      481 gatggagtgc tgactggtga tattcgcatg gctttgttgc ttgaaggaaa tgcccattac
      541 cgatgtgact tcagaactac ttacaaagct aaggagaagg gtgtcaagtt accaggctac
      601 cactttgtgg accactgcat tgagatttta agccatgaca aagattacaa caaggttaag
      661 ctgtatgagc atgctgttgc tcattctgga ttgcctgaca atgccagacg atccggtagc
      721 ggtcacggta ctggttctac tggttctggt tcttctgaga gtgcgattaa gccagacatg
      781 aagatcaacc tccgtatgga aggcaacgta aacgggcacc actttgtgat cgacggagat
      841 ggtacaggca agccttttga gggaaaacag agtatggatc ttgaagtcaa agagggcgga
      901 cctctgcctt ttgcctttga tatcctgacc actgcattcc attacggcaa cagggtattc
      961 gccgaatatc cagaccacat acaagactat tttaagcagt cgtttcctaa ggggtattcg
     1021 tgggaacgaa gcttgacttt cgaagacggg ggcatttgca ttgccagaaa cgacataaca
     1081 atggaagggg acactttcta taataaagtt cgatttcacg gtgtaaactt tcccgccaat
     1141 ggtccagtta tgcagaagaa gacgctgaaa tgggagccct ccactgagaa aatgtatgtg
     1201 cgtgatggag tgctgactgg tgatattcgc atggctttgt tgcttgaagg aaatgcccat
     1261 taccgatgtg acttcagaac tacttacaaa gctaaggaga agggtgtcaa gttaccaggc
     1321 taccactttg tggaccactg cattgagatt ttaagccatg acaaagatta caacaaggtt
     1381 aagctgtatg agcatgctgt tgctcattct ggattgcctg acaatgccag acgatccgga
     1441 ctcagatctg gcagcggtgg aggcttggag gagccggagg ccgcgacgcg gacagccgcg
     1501 gccgtggatt gcaaagacag acccgggttc ccagtgaaga ggttaataca agcccgcttg
     1561 cccttcaaac gcctgaacct tgttcccaag gagaaagttg aggaagacac atcaccaaaa
     1621 gcagctgtgg aaagcaaagt cccggatcta caattgtctt tgggcacttt tgaaagccag
     1681 tgtcacacag gctctcacgt aggcttgagt acaaagttgg tcggtggcca gggccccatt
     1741 gatagcttct tgagggcaac aatcaagcca gtaccgagtg tggtcattat cgacctgacg
     1801 gagaactgca gtgacattcc agacagccct gagggccaca gtgaactaag tcctgacact
     1861 gctggtgtgg tcaccacagt ggaaggagct gccaagcagc aggagcactc tgcagcagag
     1921 ctgtgtctcc tagagacccc ttcagacatc acttgtcaca tggaagagga gcctgggagt
     1981 ccaggagacc caaagaggac gggtgactgt caggctggtt cactacagag ctgtccggag
     2041 ctgaccccag gttctaggac atgccccacc aaggagttat caagctggag caaggcaggg
     2101 gatctcctat ttatagagaa agtgcctgtg gtggtgctag aggacatcct ggccactaag
     2161 ccctccattg cctctcttcc catgatgtcc ctggatagga gtgtcacttc agagagtgaa
     2221 atactggagt cctgccccga ggatgactcc atactgagcc actcctccac caactcctct
     2281 tctccaacca gctctcctga gggaccatcc acacccccag aacatcgggg tgggagaagc
     2341 tccccctcca ccccagcctg cagagttgcc aagaactttg tcaaaggctc cacagagaag
     2401 ggcaggagca aactgcacag agatagagaa cagcagaggg aagagaagga aaagctgagg
     2461 gaagagatac gacgagccaa ggaggaggca cggaagaaga aggaggagga gaaggagctg
     2521 aaggaaaagg agcggaggga aaagcgggag aaagatgaga aggagaaggc ggagaagcag
     2581 cggctaaagg aggaaaaacg caaggagcgg caggaagctc tggaggctaa actggaggag
     2641 aagaggaaga aggaggaaga gaagcggtta cgggaggaag agaagcggtt acgggaagaa
     2701 gagaagcgca ttaaggcaga aaaggcagag atcactcgat tcttccagaa accgaagact
     2761 ccgcaggctc ccaagaccct ggccggttcc tgtggtaagt ttgccccctt tgaaatcaaa
     2821 gaacacatgg tgctggctcc acggtgccga gctgccttgg atcaagacct gtgcgatcag
     2881 ctggaccagc tcctgcagca gcagagtgtg gcaagcacct tcctcagtga cctaaaaagc
     2941 cggctgccct tgagatcggg gcccacccgg gtctgtggcc atgacacaga catcatgaac
     3001 agggatgtgg tcattgtgga gagcagcaag gtggacggtg tgtctgaaag gaagaagttt
     3061 ggccgcatga agcttctgca gttctcagag aaccaccggc cagcatactg ggggacgtgg
     3121 aacaagaaga cagccatcat ccgcccccgg aacccctggg cccaggacaa agaccttctt
     3181 gactatgagg tagacagtga cgatgagtgg gaagaggagg aacctgggga gtctttgtcc
     3241 cacagtgagg gggatgagga tgacgatgtg ggggaagatg aggatgagga cgatggcttc
     3301 tttgtgcccc atgggtactt atctgaagat gaaggcgtga ctgaggagtg tgcagaccct
     3361 gagaaccaca aagtacacca gaagctgaag gccaaggagt gggatgagct cctagccaag
     3421 ggcaagcgct ttcgtgtgct gcagcctgtg cacgtgggat gtgtatgggc atcagaggca
     3481 gccaactgca cgagttccga cttgaagctg ctgcagcagt tcactgcatg cctgctggat
     3541 gtggcatccc ctgatgagcc agaacccggg gcctccagga gggaaaagag ggaccagcat
     3601 atcctggccc agttgcttcc gctgctacat ggcaatgtga atgggagcaa ggtgatcatc
     3661 catgaattcc aggagcaatg ccgcaggggc ctgctgaccc ttccaagtcc caccccacac
     3721 ctgcagatgc ccaacttgga ggatgctgtt gctgtgccat ccaaggccag gctgaagcgc
     3781 cttatctctg agaactctgc ctatgagaag cggcccaact tccgtatgtg ctggtatgtg
     3841 cacccagagg tgctcaagag ctttggccag gagtgcctgc cagtgccctg ccagtggacc
     3901 tacatcacca ccatgccctc agcacccagg gaagacagtg gcagtgcttc tactgagggg
     3961 cctggccaga gcacacctat gctactcaag cggaagccag cggccaccat gtgcatcacg
     4021 cagtttatga agaagcggcg ctatgatgga caggttgggt ctggggacat ggatggcttc
     4081 caggcagaca cagaagagga tgaagaggat gacacagact gtatgatcat agatgtgcca
     4141 gatgttggga gtgacgtgtc agaagctccc atacctgctc ccacactttg caagtgagga
     4201 tccaccggat ctagataact gatcataatc agccatacca cattgagaa
//
