LOCUS       sequence-88738-e        2521 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Lysosomes, Excitation: 505 / 569, Emission: 516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2521)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 2521)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdEos-CathespinB-6
FEATURES             Location/Qualifiers
     source          1..2521
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1079..1756
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="MSAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     CDS             1805..2482
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="SAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVKE
                     GGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIARN
                     DITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLLE
                     GNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPDN
                     ARR"
ORIGIN
        1 tgacgtcaga tccgctagcg ctaccggact cagatctcga gctcaagctt cgaattccag
       61 gatgtggtgg tccttgatcc ctctctcttg cctgctggca ctgaccagtg cccatgacaa
      121 gccttccttt cacccactgt cggacgacat gattaactat atcaacaaac agaatacaac
      181 atggcaggct ggacgcaact tctacaatgt tgacataagc tatctgaaga agctgtgtgg
      241 aactgtcctg ggtggaccca agctgccgga aagggttggg ttcagcgagg acataaatct
      301 acctgaatcc tttgatgcac gggaacagtg gtccaattgc ccgaccatcg cacagatcag
      361 agaccagggg tcctgtggct cttgttgggc atttggggca gtggaagcca tgtctgaccg
      421 aatctgcatt cacaccaatg gccgagtcaa tgtggaggtg tctgctgagg acctgcttac
      481 ctgctgtggt atccagtgtg gggatggctg taatggtggc tatccctctg gagcatggaa
      541 cttctggact agaaaaggcc tggtttctgg tggagtatac aattctcata taggctgctt
      601 accctacacc atccctccct gtgaacacca tgtcaatggc tcccgtcccc catgcactgg
      661 agaaggagat actcccaagt gcaacaagat gtgtgaggct ggctactcca catcctacaa
      721 ggaagataag cactatgggt acacttccta cagtgtgtct gacagcgaga aggagatcat
      781 ggcggaaatc tacaaaaatg gcccagtgga gggtgctttt actgtgtttt ctgacttctt
      841 gacttacaaa tcaggcgtat acaagcatga agccggtgat gtgatgggag gccatgccat
      901 ccgcattctg ggctggggaa tagagaatgg agtaccctac tggctggtag caaactcctg
      961 gaacgttgac tggggtgata atggtttctt taaaatcctc agaggagaga accactgtgg
     1021 aattgaatca gaaatcgtgg ctggaatccc acgcactcag gatccaccgg tcgccaccat
     1081 gagtgcgatt aagccagaca tgaagatcaa cctccgtatg gaaggcaacg taaacgggca
     1141 ccactttgtg atcgacggag atggtacagg caagcctttt gagggaaaac agagtatgga
     1201 tcttgaagtc aaagagggcg gacctctgcc ttttgccttt gatatcctga ccactgcatt
     1261 ccattacggc aacagggtat tcgccgaata tccagaccac atacaagact attttaagca
     1321 gtcgtttcct aaggggtatt cgtgggaacg aagcttgact ttcgaagacg ggggcatttg
     1381 cattgccaga aacgacataa caatggaagg ggacactttc tataataaag ttcgatttca
     1441 cggtgtaaac tttcccgcca atggtccagt tatgcagaag aagacgctga aatgggagcc
     1501 ctccactgag aaaatgtatg tgcgtgatgg agtgctgact ggtgatattc gcatggcttt
     1561 gttgcttgaa ggaaatgccc attaccgatg tgacttcaga actacttaca aagctaagga
     1621 gaagggtgtc aagttaccag gctaccactt tgtggaccac tgcattgaga ttttaagcca
     1681 tgacaaagat tacaacaagg ttaagctgta tgagcatgct gttgctcatt ctggattgcc
     1741 tgacaatgcc agacgatccg gtagcggtca cggtactggt tctactggtt ctggttcttc
     1801 tgagagtgcg attaagccag acatgaagat caacctccgt atggaaggca acgtaaacgg
     1861 gcaccacttt gtgatcgacg gagatggtac aggcaagcct tttgagggaa aacagagtat
     1921 ggatcttgaa gtcaaagagg gcggacctct gccttttgcc tttgatatcc tgaccactgc
     1981 attccattac ggcaacaggg tattcgccga atatccagac cacatacaag actattttaa
     2041 gcagtcgttt cctaaggggt attcgtggga acgaagcttg actttcgaag acgggggcat
     2101 ttgcattgcc agaaacgaca taacaatgga aggggacact ttctataata aagttcgatt
     2161 tcacggtgta aactttcccg ccaatggtcc agttatgcag aagaagacgc tgaaatggga
     2221 gccctccact gagaaaatgt atgtgcgtga tggagtgctg actggtgata ttcgcatggc
     2281 tttgttgctt gaaggaaatg cccattaccg atgtgacttc agaactactt acaaagctaa
     2341 ggagaagggt gtcaagttac caggctacca ctttgtggac cactgcattg agattttaag
     2401 ccatgacaaa gattacaaca aggttaagct gtatgagcat gctgttgctc attctggatt
     2461 gcctgacaat gccagacgat aagcggccgc gactctagat cataatcagc cataccacat
     2521 t
//
