LOCUS       sequence-88750-e        1995 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Centrosomes, Excitation: 505 / 569, Emission: 516 / 
            581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1995)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 1995)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdEos-Centrin2-N-10
FEATURES             Location/Qualifiers
     source          1..1995
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      18..27
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             570..1247
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="MSAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     CDS             1296..1973
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="SAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVKE
                     GGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIARN
                     DITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLLE
                     GNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPDN
                     ARR"
ORIGIN
        1 acgtcgatcc gctagccgcc accatggcct ccaactttaa gaaggcaaac atggcatcaa
       61 gttctcagcg aaaaagaatg agccctaagc ctgagcttac tgaagagcaa aagcaggaga
      121 tccgggaagc ttttgatctt ttcgatgcgg atggaactgg caccatagat gttaaagaac
      181 tgaaggtggc aatgagggcc ctgggctttg aacccaagaa agaagaaatt aagaaaatga
      241 taagtgaaat tgataaggaa gggacaggaa aaatgaactt tggtgacttt ttaactgtga
      301 tgacccagaa aatgtctgag aaagatacta aagaagaaat cctgaaagct ttcaagctct
      361 ttgatgatga tgaaactggg aagatttcgt tcaaaaatct gaaacgcgtg gccaaggagt
      421 tgggtgagaa cctgactgat gaggagctgc aggaaatgat tgatgaagct gatcgagatg
      481 gagatggaga ggtcagtgag caagagttcc tgcgcatcat gaaaaagacc agcctctatt
      541 cgggaagcac ggatccaccg gtcgccacca tgagtgcgat taagccagac atgaagatca
      601 acctccgtat ggaaggcaac gtaaacgggc accactttgt gatcgacgga gatggtacag
      661 gcaagccttt tgagggaaaa cagagtatgg atcttgaagt caaagagggc ggacctctgc
      721 cttttgcctt tgatatcctg accactgcat tccattacgg caacagggta ttcgccgaat
      781 atccagacca catacaagac tattttaagc agtcgtttcc taaggggtat tcgtgggaac
      841 gaagcttgac tttcgaagac gggggcattt gcattgccag aaacgacata acaatggaag
      901 gggacacttt ctataataaa gttcgatttc acggtgtaaa ctttcccgcc aatggtccag
      961 ttatgcagaa gaagacgctg aaatgggagc cctccactga gaaaatgtat gtgcgtgatg
     1021 gagtgctgac tggtgatatt cgcatggctt tgttgcttga aggaaatgcc cattaccgat
     1081 gtgacttcag aactacttac aaagctaagg agaagggtgt caagttacca ggctaccact
     1141 ttgtggacca ctgcattgag attttaagcc atgacaaaga ttacaacaag gttaagctgt
     1201 atgagcatgc tgttgctcat tctggattgc ctgacaatgc cagacgatcc ggtagcggtc
     1261 acggtactgg ttctactggt tctggttctt ctgagagtgc gattaagcca gacatgaaga
     1321 tcaacctccg tatggaaggc aacgtaaacg ggcaccactt tgtgatcgac ggagatggta
     1381 caggcaagcc ttttgaggga aaacagagta tggatcttga agtcaaagag ggcggacctc
     1441 tgccttttgc ctttgatatc ctgaccactg cattccatta cggcaacagg gtattcgccg
     1501 aatatccaga ccacatacaa gactatttta agcagtcgtt tcctaagggg tattcgtggg
     1561 aacgaagctt gactttcgaa gacgggggca tttgcattgc cagaaacgac ataacaatgg
     1621 aaggggacac tttctataat aaagttcgat ttcacggtgt aaactttccc gccaatggtc
     1681 cagttatgca gaagaagacg ctgaaatggg agccctccac tgagaaaatg tatgtgcgtg
     1741 atggagtgct gactggtgat attcgcatgg ctttgttgct tgaaggaaat gcccattacc
     1801 gatgtgactt cagaactact tacaaagcta aggagaaggg tgtcaagtta ccaggctacc
     1861 actttgtgga ccactgcatt gagattttaa gccatgacaa agattacaac aaggttaagc
     1921 tgtatgagca tgctgttgct cattctggat tgcctgacaa tgccagacga taagcggccg
     1981 cgactctaga tcata
//
