LOCUS       sequence-88751-e        2037 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Centromeres, Excitation: 505 / 569, Emission: 
            516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2037)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 2037)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdEos-CENPB-N-22
FEATURES             Location/Qualifiers
     source          1..2037
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      16..25
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    529..581
                     /label=MCS
                     /note="multiple cloning site"
     CDS             595..1272
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="MSAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     CDS             1321..1998
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="SAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVKE
                     GGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIARN
                     DITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLLE
                     GNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPDN
                     ARR"
ORIGIN
        1 acgtcagatc cgctagccac catgggcccc aagcggcggc agctgacgtt ccgggagaag
       61 tcgcggatca tccaggaggt ggaggagaac ccggacctac gcaagggcga gatcgcgcgg
      121 cgcttcaaca tcccgccgtc cacgctgagc acgatcctga agaacaagcg cgccatcctg
      181 gcgtcggagc gcaagtacgg ggtggcctcc acctgccgca agaccaacaa gctgtctccc
      241 tacgacaagc tcgagggctt gctcatcgcc tggttccagc agatccgcgc cgccggcctg
      301 ccggtcaagg gcatcatcct caaggagaag gcgctgcgca tagccgagga gctgggcatg
      361 gacgacttca ccgcctccaa cggctggctg gaccgcttcc gccggcgcca cggcgtggtg
      421 tcctgcagcg gcgtggcccg cgcccgcgcg cgaaacgctg ccccccgcac cccggcggcg
      481 cctgccagtc cggccgcggt gccctcggag ggcagtggcg ggagcactct cgagctcaag
      541 cttcgaattc tgcagtcgac ggtaccgcgg gcccgggatc caccggtcgc caccatgagt
      601 gcgattaagc cagacatgaa gatcaacctc cgtatggaag gcaacgtaaa cgggcaccac
      661 tttgtgatcg acggagatgg tacaggcaag ccttttgagg gaaaacagag tatggatctt
      721 gaagtcaaag agggcggacc tctgcctttt gcctttgata tcctgaccac tgcattccat
      781 tacggcaaca gggtattcgc cgaatatcca gaccacatac aagactattt taagcagtcg
      841 tttcctaagg ggtattcgtg ggaacgaagc ttgactttcg aagacggggg catttgcatt
      901 gccagaaacg acataacaat ggaaggggac actttctata ataaagttcg atttcacggt
      961 gtaaactttc ccgccaatgg tccagttatg cagaagaaga cgctgaaatg ggagccctcc
     1021 actgagaaaa tgtatgtgcg tgatggagtg ctgactggtg atattcgcat ggctttgttg
     1081 cttgaaggaa atgcccatta ccgatgtgac ttcagaacta cttacaaagc taaggagaag
     1141 ggtgtcaagt taccaggcta ccactttgtg gaccactgca ttgagatttt aagccatgac
     1201 aaagattaca acaaggttaa gctgtatgag catgctgttg ctcattctgg attgcctgac
     1261 aatgccagac gatccggtag cggtcacggt actggttcta ctggttctgg ttcttctgag
     1321 agtgcgatta agccagacat gaagatcaac ctccgtatgg aaggcaacgt aaacgggcac
     1381 cactttgtga tcgacggaga tggtacaggc aagccttttg agggaaaaca gagtatggat
     1441 cttgaagtca aagagggcgg acctctgcct tttgcctttg atatcctgac cactgcattc
     1501 cattacggca acagggtatt cgccgaatat ccagaccaca tacaagacta ttttaagcag
     1561 tcgtttccta aggggtattc gtgggaacga agcttgactt tcgaagacgg gggcatttgc
     1621 attgccagaa acgacataac aatggaaggg gacactttct ataataaagt tcgatttcac
     1681 ggtgtaaact ttcccgccaa tggtccagtt atgcagaaga agacgctgaa atgggagccc
     1741 tccactgaga aaatgtatgt gcgtgatgga gtgctgactg gtgatattcg catggctttg
     1801 ttgcttgaag gaaatgccca ttaccgatgt gacttcagaa ctacttacaa agctaaggag
     1861 aagggtgtca agttaccagg ctaccacttt gtggaccact gcattgagat tttaagccat
     1921 gacaaagatt acaacaaggt taagctgtat gagcatgctg ttgctcattc tggattgcct
     1981 gacaatgcca gacgataagc ggccgcgact ctagatcata atcagccata ccacatt
//
