LOCUS       sequence-88752-e        3119 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane/Vesicles, Excitation: 505 / 569, Emission: 
            516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3119)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 3119)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdEos-C-Src-7
FEATURES             Location/Qualifiers
     source          1..3119
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             56..97
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     CDS             1676..2353
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="MSAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     CDS             2402..3079
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="SAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVKE
                     GGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIARN
                     DITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLLE
                     GNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPDN
                     ARR"
ORIGIN
        1 acgtcagatc cgctagcgct accggactca gatctcgagc tcaagcttcg aattcatggg
       61 gagcagcaag agcaagccca aggaccccag ccagcgccgg cgcagcctgg agccacccga
      121 cagcacccac cacgggggat tcccagcctc gcagaccccc aacaagacag cagcccccga
      181 cacgcaccgc acccccagcc gctcctttgg gaccgtggcc accgagccca agctcttcgg
      241 gggcttcaac acttctgaca ccgtcacgtc gccgcagcgt gccggggcac tggctggcgg
      301 cgtcaccact ttcgtggctc tctatgacta cgagtcccgg actgaaacgg acttgtcctt
      361 caagaaagga gaacgcctgc agattgtcaa caacacggaa ggtgactggt ggctggctca
      421 ttccctcact acaggacaga cgggctacat ccccagtaac tatgtcgcgc cctcagactc
      481 catccaggct gaagagtggt actttgggaa gatcactcgt cgggagtccg agcggctgct
      541 gctcaacccc gaaaaccccc ggggaacctt cttggtccgg gagagcgaga cgacaaaagg
      601 tgcctattgc ctctccgttt ctgactttga caacgccaag gggctcaatg tgaagcacta
      661 caagatccgc aagctggaca gcggcggctt ctacatcacc tcacgcacac agttcagcag
      721 cctgcagcag ctggtggcct actactccaa acatgctgat ggcttgtgcc accgcctgac
      781 caacgtctgc cccacgtcca agccccagac ccagggactc gccaaggacg cgtgggaaat
      841 cccccgggag tcgctgcggc tggaggtgaa gctggggcag ggctgctttg gagaggtctg
      901 gatggggacc tggaacggca ccaccagagt ggccataaag actctgaagc ccggcaccat
      961 gtccccggag gccttcctgc aggaagccca agtgatgaag aagctccggc atgagaagct
     1021 ggttcagctg tacgcagtgg tgtcggaaga gcccatctac atcgtcactg agtacatgag
     1081 caaggggagc ctcctggatt tcctgaaggg agagatgggc aagtacctgc ggctgccaca
     1141 gctcgtcgat atggctgctc agattgcatc cggcatggcc tatgtggaga ggatgaacta
     1201 cgtgcaccga gacctgcggg cggccaacat cctggtgggg gagaacctgg tgtgcaaggt
     1261 ggctgacttt gggctggcac gcctcatcga ggacaacgag tacacagcac ggcaaggtgc
     1321 caagttcccc atcaagtgga cagcccccga ggcagccctc tatggccggt tcaccatcaa
     1381 gtcggatgtc tggtccttcg gcatcctgct gactgagctg accaccaagg gccgggtgcc
     1441 atacccaggg atggtcaaca gggaggtgct ggaccaggtg gagaggggct accgcatgcc
     1501 ctgcccgccc gagtgccccg agtcgctgca tgacctcatg tgccagtgct ggcggaagga
     1561 ccctgaggag cggcccactt ttgagtacct gcaggccttc ctggaggact acttcacctc
     1621 gacagagccc cagtaccagc ctggagagaa cctacgggat ccaccggtcg ccaccatgag
     1681 tgcgattaag ccagacatga agatcaacct ccgtatggaa ggcaacgtaa acgggcacca
     1741 ctttgtgatc gacggagatg gtacaggcaa gccttttgag ggaaaacaga gtatggatct
     1801 tgaagtcaaa gagggcggac ctctgccttt tgcctttgat atcctgacca ctgcattcca
     1861 ttacggcaac agggtattcg ccgaatatcc agaccacata caagactatt ttaagcagtc
     1921 gtttcctaag gggtattcgt gggaacgaag cttgactttc gaagacgggg gcatttgcat
     1981 tgccagaaac gacataacaa tggaagggga cactttctat aataaagttc gatttcacgg
     2041 tgtaaacttt cccgccaatg gtccagttat gcagaagaag acgctgaaat gggagccctc
     2101 cactgagaaa atgtatgtgc gtgatggagt gctgactggt gatattcgca tggctttgtt
     2161 gcttgaagga aatgcccatt accgatgtga cttcagaact acttacaaag ctaaggagaa
     2221 gggtgtcaag ttaccaggct accactttgt ggaccactgc attgagattt taagccatga
     2281 caaagattac aacaaggtta agctgtatga gcatgctgtt gctcattctg gattgcctga
     2341 caatgccaga cgatccggta gcggtcacgg tactggttct actggttctg gttcttctga
     2401 gagtgcgatt aagccagaca tgaagatcaa cctccgtatg gaaggcaacg taaacgggca
     2461 ccactttgtg atcgacggag atggtacagg caagcctttt gagggaaaac agagtatgga
     2521 tcttgaagtc aaagagggcg gacctctgcc ttttgccttt gatatcctga ccactgcatt
     2581 ccattacggc aacagggtat tcgccgaata tccagaccac atacaagact attttaagca
     2641 gtcgtttcct aaggggtatt cgtgggaacg aagcttgact ttcgaagacg ggggcatttg
     2701 cattgccaga aacgacataa caatggaagg ggacactttc tataataaag ttcgatttca
     2761 cggtgtaaac tttcccgcca atggtccagt tatgcagaag aagacgctga aatgggagcc
     2821 ctccactgag aaaatgtatg tgcgtgatgg agtgctgact ggtgatattc gcatggcttt
     2881 gttgcttgaa ggaaatgccc attaccgatg tgacttcaga actacttaca aagctaagga
     2941 gaagggtgtc aagttaccag gctaccactt tgtggaccac tgcattgaga ttttaagcca
     3001 tgacaaagat tacaacaagg ttaagctgta tgagcatgct gttgctcatt ctggattgcc
     3061 tgacaatgcc agacgataag cggccgcgac tctagatcat aatcagccat accacattg
//
