LOCUS       sequence-88758-e        2232 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane, Excitation: 505 / 569, Emission: 516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2232)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 2232)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdEos-Dectin1A-N-10
FEATURES             Location/Qualifiers
     source          1..2232
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      13..22
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             790..1467
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="MSAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     CDS             1516..2193
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="SAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVKE
                     GGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIARN
                     DITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLLE
                     GNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPDN
                     ARR"
ORIGIN
        1 agatccgcta gcgccaccat ggaatatcat cctgatttag aaaatttgga tgaagatgga
       61 tatactcaat tacacttcga ctctcaaagc aataccagga tagctgttgt ttcagagaaa
      121 ggatcgtgtg ctgcatctcc tccttggcgc ctcattgctg taattttggg aatcctatgc
      181 ttggtaatac tggtgatagc tgtggtcctg ggtaccatgg ctatttggag atccaattca
      241 ggaagcaaca cattggagaa tggctacttt ctatcaagaa ataaagagaa ccacagtcaa
      301 cccacacaat catctttaga agacagtgtg actcctacca aagctgtcaa aaccacaggg
      361 gttctttcca gcccttgtcc tcctaattgg attatatatg agaagagctg ttatctattc
      421 agcatgtcac taaattcctg ggatggaagt aaaagacaat gctggcaact gggctctaat
      481 ctcctaaaga tagacagctc aaatgaattg ggatttatag taaaacaagt gtcttcccaa
      541 cctgataatt cattttggat aggcctttct cggccccaga ctgaagtacc atggctctgg
      601 gaggatggat caacattctc ttctaactta tttcagatca gaaccacagc tacccaagaa
      661 aacccatctc caaattgtgt atggattcac gtgtcagtca tttatgacca actgtgtagt
      721 gtgccctcat atagtatttg tgagaagaag ttttcaatga gctccggagg ggatccaccg
      781 gtcgccacca tgagtgcgat taagccagac atgaagatca acctccgtat ggaaggcaac
      841 gtaaacgggc accactttgt gatcgacgga gatggtacag gcaagccttt tgagggaaaa
      901 cagagtatgg atcttgaagt caaagagggc ggacctctgc cttttgcctt tgatatcctg
      961 accactgcat tccattacgg caacagggta ttcgccgaat atccagacca catacaagac
     1021 tattttaagc agtcgtttcc taaggggtat tcgtgggaac gaagcttgac tttcgaagac
     1081 gggggcattt gcattgccag aaacgacata acaatggaag gggacacttt ctataataaa
     1141 gttcgatttc acggtgtaaa ctttcccgcc aatggtccag ttatgcagaa gaagacgctg
     1201 aaatgggagc cctccactga gaaaatgtat gtgcgtgatg gagtgctgac tggtgatatt
     1261 cgcatggctt tgttgcttga aggaaatgcc cattaccgat gtgacttcag aactacttac
     1321 aaagctaagg agaagggtgt caagttacca ggctaccact ttgtggacca ctgcattgag
     1381 attttaagcc atgacaaaga ttacaacaag gttaagctgt atgagcatgc tgttgctcat
     1441 tctggattgc ctgacaatgc cagacgatcc ggtagcggtc acggtactgg ttctactggt
     1501 tctggttctt ctgagagtgc gattaagcca gacatgaaga tcaacctccg tatggaaggc
     1561 aacgtaaacg ggcaccactt tgtgatcgac ggagatggta caggcaagcc ttttgaggga
     1621 aaacagagta tggatcttga agtcaaagag ggcggacctc tgccttttgc ctttgatatc
     1681 ctgaccactg cattccatta cggcaacagg gtattcgccg aatatccaga ccacatacaa
     1741 gactatttta agcagtcgtt tcctaagggg tattcgtggg aacgaagctt gactttcgaa
     1801 gacgggggca tttgcattgc cagaaacgac ataacaatgg aaggggacac tttctataat
     1861 aaagttcgat ttcacggtgt aaactttccc gccaatggtc cagttatgca gaagaagacg
     1921 ctgaaatggg agccctccac tgagaaaatg tatgtgcgtg atggagtgct gactggtgat
     1981 attcgcatgg ctttgttgct tgaaggaaat gcccattacc gatgtgactt cagaactact
     2041 tacaaagcta aggagaaggg tgtcaagtta ccaggctacc actttgtgga ccactgcatt
     2101 gagattttaa gccatgacaa agattacaac aaggttaagc tgtatgagca tgctgttgct
     2161 cattctggat tgcctgacaa tgccagacga taagcggccg cgactctaga tcataatcag
     2221 ccataccaca tt
//
