LOCUS       sequence-8876-ex        1750 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Kaiser SE, Brickner JH, Reilein AR, Fenn TD, Walter P, Brunger AT
  TITLE     Structural basis of FFAT motif-mediated ER targeting.
  JOURNAL   Structure. 2005 Jul . 13(7):1035-45.
  PUBMED    16004875
REFERENCE   2  (bases 1 to 1750)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Structure. 
            2005 Jul . 13(7):1035-45."
COMMENT     Sequence Label: pEGFP-N1-VAPA(1-242) K87D/M89D
FEATURES             Location/Qualifiers
     source          1..1750
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        422..625
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     576..596
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      1453..1462
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(1504..1525)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 tctgcttgct gctttgctca catttcttcc tgcgtatccc tgatcgtgat acgtataccg
       61 ccatgcatta gtatatagta tcattacggg tcatagtcat agcccatata tggagtccgc
      121 gtacataact acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
      181 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
      241 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
      301 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
      361 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
      421 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
      481 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
      541 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
      601 gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct agcgctaccg
      661 gactcagatc tcgagatggc gaaacacgag caaatcctgg tcctcgaccc tccttcagac
      721 ctcaaattca aaggcccctt cacagatgta gtcactacaa atcttaaatt gcagaatcca
      781 tcggacagaa aagtgtgttt caaagtgaag accacagcgc cccgccggta ctgcgtgcgg
      841 cccaacagtg gggttattga cccagggtca atcgtgactg tctcagtaat gctgcagccc
      901 tttgactatg atccgaacga aaagagtaaa catgacttcg acgtacagac aatttttgct
      961 ccaccaaaca tttcagatat ggaagctgtg tggaaagagg caaaacctga cgaactaatg
     1021 gattctaaac tgagatgtgt atttgagatg ccgaatgaaa acgataagct gaacgacatg
     1081 gaacctagca aagctgtccc actgaatgca tccaagcaag atggacccct gccaaaacca
     1141 catagtgttt cactcaatga cacggagaca aggaaactga tggaagagtg caagcgactt
     1201 cagggagaaa tgatgaagct ctcagaagaa aaccgacacc tgagagatga aggcctacgg
     1261 ctcagaaagg tagcacattc ggataaacct ggatccacct cagccgtgtc cttcagagat
     1321 aatgtcacca gtcctcttcc ttctcttctg gttgtaattg cagccatttt cattggattc
     1381 tttctaggga aattcatctc gaagcttcga attctgcagt cgacggtacc gcgggcccgg
     1441 gatccaccgg tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
     1501 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
     1561 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
     1621 cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc
     1681 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
     1741 caggagcgca
//
