LOCUS       sequence-88761-e        2311 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: MT End Binding Protein, Excitation: 505 / 569, 
            Emission: 516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2311)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 2311)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdEos-EB1-N-18
FEATURES             Location/Qualifiers
     source          1..2311
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      7..16
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             871..1548
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="MSAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     CDS             1597..2274
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="SAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVKE
                     GGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIARN
                     DITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLLE
                     GNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPDN
                     ARR"
ORIGIN
        1 gctagcgcca ccatggcagt gaacgtatac tcaacgtcag tgaccagtga taacctaagt
       61 cgacatgaca tgctggcctg gatcaatgag tctctgcagt tgaatctgac aaagatcgaa
      121 cagttgtgct caggggctgc gtattgtcag tttatggaca tgctgttccc tggctccatt
      181 gccttgaaga aagtgaaatt ccaagctaag ctagaacacg agtacatcca gaacttcaaa
      241 atattacaag caggttttaa gagaatgggt gttgacaaaa taattcctgt ggacaaatta
      301 gtaaaaggaa agtttcagga caattttgaa ttcgttcagt ggttcaagaa gttcttcgat
      361 gcaaactatg atggaaaaga ctatgaccct gtggctgcta gacaaggtca agaaactgca
      421 gtggctcctt cccttgttgc tccagctctg aataaaccga agaaacctct cacttctagc
      481 agtgcagctc cccagaggcc catctcaaca cagagaaccg ctgcggctcc taaggctggc
      541 cctggtgtgg tgcgaaagaa ccctggtgtg ggcaacggag acgacgaggc agctgagttg
      601 atgcagcagg tcaacgtatt gaaacttact gttgaagact tggagaaaga gagggatttc
      661 tacttcggaa agctacggaa cattgaattg atttgccagg agaacgaggg ggaaaacgac
      721 cctgtattgc agaggattgt agacattctg tatgccacag atgaaggctt tgtgatacct
      781 gatgaagggg gcccacagga ggagcaagaa gagtatagca gcggtggagc aagcgcagcc
      841 agtggtagcg cggatccacc ggtcgccacc atgagtgcga ttaagccaga catgaagatc
      901 aacctccgta tggaaggcaa cgtaaacggg caccactttg tgatcgacgg agatggtaca
      961 ggcaagcctt ttgagggaaa acagagtatg gatcttgaag tcaaagaggg cggacctctg
     1021 ccttttgcct ttgatatcct gaccactgca ttccattacg gcaacagggt attcgccgaa
     1081 tatccagacc acatacaaga ctattttaag cagtcgtttc ctaaggggta ttcgtgggaa
     1141 cgaagcttga ctttcgaaga cgggggcatt tgcattgcca gaaacgacat aacaatggaa
     1201 ggggacactt tctataataa agttcgattt cacggtgtaa actttcccgc caatggtcca
     1261 gttatgcaga agaagacgct gaaatgggag ccctccactg agaaaatgta tgtgcgtgat
     1321 ggagtgctga ctggtgatat tcgcatggct ttgttgcttg aaggaaatgc ccattaccga
     1381 tgtgacttca gaactactta caaagctaag gagaagggtg tcaagttacc aggctaccac
     1441 tttgtggacc actgcattga gattttaagc catgacaaag attacaacaa ggttaagctg
     1501 tatgagcatg ctgttgctca ttctggattg cctgacaatg ccagacgatc cggtagcggt
     1561 cacggtactg gttctactgg ttctggttct tctgagagtg cgattaagcc agacatgaag
     1621 atcaacctcc gtatggaagg caacgtaaac gggcaccact ttgtgatcga cggagatggt
     1681 acaggcaagc cttttgaggg aaaacagagt atggatcttg aagtcaaaga gggcggacct
     1741 ctgccttttg cctttgatat cctgaccact gcattccatt acggcaacag ggtattcgcc
     1801 gaatatccag accacataca agactatttt aagcagtcgt ttcctaaggg gtattcgtgg
     1861 gaacgaagct tgactttcga agacgggggc atttgcattg ccagaaacga cataacaatg
     1921 gaaggggaca ctttctataa taaagttcga tttcacggtg taaactttcc cgccaatggt
     1981 ccagttatgc agaagaagac gctgaaatgg gagccctcca ctgagaaaat gtatgtgcgt
     2041 gatggagtgc tgactggtga tattcgcatg gctttgttgc ttgaaggaaa tgcccattac
     2101 cgatgtgact tcagaactac ttacaaagct aaggagaagg gtgtcaagtt accaggctac
     2161 cactttgtgg accactgcat tgagatttta agccatgaca aagattacaa caaggttaag
     2221 ctgtatgagc atgctgttgc tcattctgga ttgcctgaca atgccagacg ataagcggcc
     2281 gcgactctag atcataatca gccataccac a
//
