LOCUS       sequence-88764-e        4685 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 
            / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4685)
  AUTHORS   Kanchanawong P, Shtengel G, Pasapera AM, Ramko EB, Davidson MW, Hess
            HF, Waterman CM
  TITLE     Nanoscale architecture of integrin-based cell adhesions.
  JOURNAL   Nature. 2010 Nov 25;468(7323):580-4. doi: 10.1038/nature09621.
  PUBMED    21107430
REFERENCE   2  (bases 1 to 4685)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2010-11-25- 25"; volume: "468"; issue: "7323"; 
            pages: "580-4"
COMMENT     Sequence Label: tdEos-FAK-5
FEATURES             Location/Qualifiers
     source          1..4685
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             35..712
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="MSAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     CDS             761..1435
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="SAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVKE
                     GGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIARN
                     DITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLLE
                     GNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPDN
                     ARR"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggtcgc caccatgagt gcgattaagc cagacatgaa
       61 gatcaacctc cgtatggaag gcaacgtaaa cgggcaccac tttgtgatcg acggagatgg
      121 tacaggcaag ccttttgagg gaaaacagag tatggatctt gaagtcaaag agggcggacc
      181 tctgcctttt gcctttgata tcctgaccac tgcattccat tacggcaaca gggtattcgc
      241 cgaatatcca gaccacatac aagactattt taagcagtcg tttcctaagg ggtattcgtg
      301 ggaacgaagc ttgactttcg aagacggggg catttgcatt gccagaaacg acataacaat
      361 ggaaggggac actttctata ataaagttcg atttcacggt gtaaactttc ccgccaatgg
      421 tccagttatg cagaagaaga cgctgaaatg ggagccctcc actgagaaaa tgtatgtgcg
      481 tgatggagtg ctgactggtg atattcgcat ggctttgttg cttgaaggaa atgcccatta
      541 ccgatgtgac ttcagaacta cttacaaagc taaggagaag ggtgtcaagt taccaggcta
      601 ccactttgtg gaccactgca ttgagatttt aagccatgac aaagattaca acaaggttaa
      661 gctgtatgag catgctgttg ctcattctgg attgcctgac aatgccagac gatccggtag
      721 cggtcacggt actggttcta ctggttctgg ttcttctgag agtgcgatta agccagacat
      781 gaagatcaac ctccgtatgg aaggcaacgt aaacgggcac cactttgtga tcgacggaga
      841 tggtacaggc aagccttttg agggaaaaca gagtatggat cttgaagtca aagagggcgg
      901 acctctgcct tttgcctttg atatcctgac cactgcattc cattacggca acagggtatt
      961 cgccgaatat ccagaccaca tacaagacta ttttaagcag tcgtttccta aggggtattc
     1021 gtgggaacga agcttgactt tcgaagacgg gggcatttgc attgccagaa acgacataac
     1081 aatggaaggg gacactttct ataataaagt tcgatttcac ggtgtaaact ttcccgccaa
     1141 tggtccagtt atgcagaaga agacgctgaa atgggagccc tccactgaga aaatgtatgt
     1201 gcgtgatgga gtgctgactg gtgatattcg catggctttg ttgcttgaag gaaatgccca
     1261 ttaccgatgt gacttcagaa ctacttacaa agctaaggag aagggtgtca agttaccagg
     1321 ctaccacttt gtggaccact gcattgagat tttaagccat gacaaagatt acaacaaggt
     1381 taagctgtat gagcatgctg ttgctcattc tggattgcct gacaatgcca gacgatccgg
     1441 actcagatct atggcagcag cttaccttga tccaaacttg aatcatacac caagttcaag
     1501 tgcaaagacg cacctcggta ctgggatgga gcgttccccg ggggccatgg agcgagtcct
     1561 aaaggttttt cactactttg aaaacagcag cgagccaacg acgtgggcca gcattatccg
     1621 gcatggagat gctactgatg ttcgaggcat aatacagaag attgtggact gtcacaaagt
     1681 gaaaaatgtg gcctgctatg ggttgcgact cagtcatctg cagtctgagg aggttcactg
     1741 gctgcacctg gacatggggg tatccaatgt gagagagaaa tttgaactag cacatcctcc
     1801 agaagaatgg aaatatgaac tgagaattcg gtacctgccc aaaggatttc taaaccagtt
     1861 cactgaggac aaaccaactt taaatttttt ctatcagcag gtgaaaaatg actatatgtt
     1921 agaaatagca gatcaagtgg accaggaaat tgctttgaaa ctaggttgcc ttgaaatcag
     1981 gagatcctac ggagagatga gaggcaatgc attagagaag aaatccaact atgaagtgct
     2041 agaaaaagat gtcggtttaa gacgattttt tccgaagagt ttgctagatt cagtgaaggc
     2101 caaaacacta cgaaaattaa tccaacagac atttcgacaa tttgccaacc tcaacagaga
     2161 agaaagtatt ttgaaattct ttgagatcct ctctccagtg tacagatttg acaaggaatg
     2221 cttcaagtgt gcccttggtt caagctggat tatttcagtg gagctggcaa ttggcccaga
     2281 ggaaggaatc agctacctta cagacaaggg tgcaaatcca actcacctgg cagattttaa
     2341 tcaagtacaa actattcagt attcaaacag tgaagacaag gacagaaaag ggatgttgca
     2401 actgaagata gctggtgcac ctgagcctct gacagtgaca gcaccatcct taaccattgc
     2461 agagaatatg gctgacttga tagacggata ctgccgactg gtgaatggag ccacgcaatc
     2521 ttttattatc aggccacaga aagaaggtga aagagcttta ccatcaatac caaagctggc
     2581 caacaatgag aagcaaggag taaggtcgca cacagtctct gtatcagaaa cagatgacta
     2641 tgcagagata atagatgaag aagatactta tacaatgcca tcaaccagag attatgaaat
     2701 tcaaagggag agaattgaac tggggcgctg cattggtgaa ggacagtttg gagatgtgca
     2761 ccaaggaatt tacatgagtc cggaaaatcc agctatggct gtagcaatca aaacatgtaa
     2821 aaactgcacc tcagacagcg ttagagaaaa gttcctacaa gaagccttaa caatgcgtca
     2881 gtttgatcat cctcacattg tgaagctcat tggagttatt acagaaaacc cagtgtggat
     2941 aatcatggag ctctgtacac ttggagagtt gagatcgttt ctgcaagtaa gaaaattcag
     3001 cttggacctg gcctccctca tcctctacgc ttaccagctt agcacagcac ttgcttacct
     3061 agagagcaaa agatttgtac atagagatat tgctgctagg aacgtgctgg tatctgccac
     3121 tgactgtgtg aaattgggtg actttggctt atcccgatac atggaagaca gtacttacta
     3181 taaagcttcc aaaggaaagt tacctatcaa atggatggct ccagagtcaa tcaacttccg
     3241 acggtttacc tcagcaagcg atgtgtggat gtttggtgtg tgtatgtggg agatcctgat
     3301 gcatggggta aagcccttcc agggagtgaa aaataatgat gttattggtc ggattgagaa
     3361 cggtgagcgg ctccccatgc ctccgaactg ccctcccacc ctctacagcc ttatgaccaa
     3421 gtgctgggca tacgacccta gtagacgacc caggtttact gaacttaaag cacaactcag
     3481 tacaatactg gaggaggaga agctgcagca agaggaacga atgagaatgg aatccaggcg
     3541 acaagtcaca gtatcctggg actcaggagg atcagatgaa gctcctccca agcccagcag
     3601 gcctggttac cccagcccaa ggtccagtga agggttttat ccgagtcctc agcatatggt
     3661 acagccaaat cactaccagg tatctggcta ctctggttct catgggatac cagccatggc
     3721 aggcagcatt tatcctgggc aagcttctct cttggatcaa acagattcct ggaaccatcg
     3781 acctcaggaa gtatcagcat ggcagccaaa catggaggat tcgggcactt tggatgtacg
     3841 aggaatgggg caggttctgc ccacacatct catggaggag aggttaataa gacaacagca
     3901 agagatggaa gaagatcaac gctggcttga gaaagaggaa cgattcctgg taatgaaacc
     3961 tgatgtgcgg ctctccagag gcagcattga acgggaggac ggaggtctcc agggcccagc
     4021 tggtaaccag cacatatatc agcctgtggg taaaccagat catgccgctc caccaaagaa
     4081 gccccctcgc cctggagccc cccacttggg cagcctcgcg agcctgaaca gccccgtgga
     4141 cagctacaac gaaggcgtga agatcaagcc acaggaaatc agccctcctc ctacggccaa
     4201 cctggaccgc tccaatgaca aagtctatga gaatgtaacc gggctggtga aagctgtcat
     4261 agagatgtcc agtaaaatac agccagctcc gccagaggag tacgtgccca tggtaaagga
     4321 ggttggcttg gcgctgagaa ccttgctagc aacagtggat gagtcgctgc cagtgcttcc
     4381 tgcaagcacc cacagagaga ttgagatggc ccagaaactg ctgaactctg acctggctga
     4441 gctcattaac aagatgaagc tggcccagca gtacgtcatg accagcctgc agcaggagta
     4501 caagaagcaa atgctgacgg ctgctcacgc tctggctgtg gatgccaaga acttgctgga
     4561 tgtcatcgat caagccagac tgaaaatgat cagccagtcc aggcccacta agctgccgta
     4621 gccgaattct gcagatggga tccaccggat ctagataact gatcataatc agccatacca
     4681 cattg
//
