LOCUS       sequence-88766-e        2468 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleoli, Excitation: 505 / 569, Emission: 516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2468)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 2468)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdEos-Fibrillarin-7
FEATURES             Location/Qualifiers
     source          1..2468
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             34..711
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="MSAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     CDS             760..1434
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="SAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVKE
                     GGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIARN
                     DITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLLE
                     GNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPDN
                     ARR"
     CDS             complement(2230..2241)
                     /codon_start=1
                     /gene="cspA"
                     /product="translation enhancing element for E. coli (Qing 
                     et al., 2004)"
                     /label=TEE
                     /translation="NHKV"
ORIGIN
        1 acgtcagatc cgctagcgct accggtcgcc accatgagtg cgattaagcc agacatgaag
       61 atcaacctcc gtatggaagg caacgtaaac gggcaccact ttgtgatcga cggagatggt
      121 acaggcaagc cttttgaggg aaaacagagt atggatcttg aagtcaaaga gggcggacct
      181 ctgccttttg cctttgatat cctgaccact gcattccatt acggcaacag ggtattcgcc
      241 gaatatccag accacataca agactatttt aagcagtcgt ttcctaaggg gtattcgtgg
      301 gaacgaagct tgactttcga agacgggggc atttgcattg ccagaaacga cataacaatg
      361 gaaggggaca ctttctataa taaagttcga tttcacggtg taaactttcc cgccaatggt
      421 ccagttatgc agaagaagac gctgaaatgg gagccctcca ctgagaaaat gtatgtgcgt
      481 gatggagtgc tgactggtga tattcgcatg gctttgttgc ttgaaggaaa tgcccattac
      541 cgatgtgact tcagaactac ttacaaagct aaggagaagg gtgtcaagtt accaggctac
      601 cactttgtgg accactgcat tgagatttta agccatgaca aagattacaa caaggttaag
      661 ctgtatgagc atgctgttgc tcattctgga ttgcctgaca atgccagacg atccggtagc
      721 ggtcacggta ctggttctac tggttctggt tcttctgaga gtgcgattaa gccagacatg
      781 aagatcaacc tccgtatgga aggcaacgta aacgggcacc actttgtgat cgacggagat
      841 ggtacaggca agccttttga gggaaaacag agtatggatc ttgaagtcaa agagggcgga
      901 cctctgcctt ttgcctttga tatcctgacc actgcattcc attacggcaa cagggtattc
      961 gccgaatatc cagaccacat acaagactat tttaagcagt cgtttcctaa ggggtattcg
     1021 tgggaacgaa gcttgacttt cgaagacggg ggcatttgca ttgccagaaa cgacataaca
     1081 atggaagggg acactttcta taataaagtt cgatttcacg gtgtaaactt tcccgccaat
     1141 ggtccagtta tgcagaagaa gacgctgaaa tgggagccct ccactgagaa aatgtatgtg
     1201 cgtgatggag tgctgactgg tgatattcgc atggctttgt tgcttgaagg aaatgcccat
     1261 taccgatgtg acttcagaac tacttacaaa gctaaggaga agggtgtcaa gttaccaggc
     1321 taccactttg tggaccactg cattgagatt ttaagccatg acaaagatta caacaaggtt
     1381 aagctgtatg agcatgctgt tgctcattct ggattgcctg acaatgccag acgatccgga
     1441 ctcagatctc gagccatgaa gccaggattc agtccccgtg ggggtggctt tggcggccga
     1501 gggggctttg gtgaccgtgg tggtcgtgga ggccgagggg gctttggcgg gggccgaggc
     1561 caaggcggag gctttagagg tcgtggacga ggaggaggtg gaggcggcgg cggcggtgga
     1621 ggaggaggaa gaggtggtgg aggcttccat tttggtggca accggggtcg tggtcgggga
     1681 ggaaaaagag gaaaccagtc ggggaagaat gtgatggtgg agccgcatcg gcatgagggt
     1741 gtcttcattt gtcggggaaa ggaagatgca ctggtcacca agaacctggt ccctggggaa
     1801 tcagtttatg gagagaagag agtctcgatt tcggaaggag atgacaaaat tgagtaccga
     1861 gcctggaacc ccttccgctc caagcttgca gcagcaatcc tgggtggtgt ggaccagatc
     1921 cacatcaaac cgggggctaa ggttctctac ctcggggctg cctcgggcac cacggtctcc
     1981 catgtctctg acatcgttgg tccggatggt ctagtctatg cagtcgagtt ctcccaccgc
     2041 tctggccgtg acctcattaa cttggccaag aagaggacca acatcattcc tgtgatcgag
     2101 gatgctcgac acccacacaa ataccgcatg ctcatcgcaa tggtggatgt gatctttgct
     2161 gatgtggccc agccagacca gacccggatt gtggccctga atgcccacac cttcctgcgt
     2221 aatggaggac actttgtgat ttccattaag gccaactgca ttgactccac agcctcagcc
     2281 gaggccgtgt ttgcctccga agtgaaaaag atgcaacagg agaacatgaa gccgcaggag
     2341 cagttgaccc ttgagccata tgaaagagac catgccgtgg tcgtgggagt gtacaggcca
     2401 ccccccaagg tgaagaactg aggatccacc ggatctagat aactgatcat aatcagccat
     2461 accacatt
//
