LOCUS       sequence-88768-e        9409 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin/Focal Adhesions, Excitation: 505 / 569, 
            Emission: 516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 9409)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 9409)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdEos-FilaminA-N-9
FEATURES             Location/Qualifiers
     source          1..9409
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7967..8644
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="MSAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     CDS             8693..9370
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="SAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVKE
                     GGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIARN
                     DITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLLE
                     GNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPDN
                     ARR"
ORIGIN
        1 cgtcagatcc gctagcgcca ccatgagtag ctcccactct cgggcgggcc agagcgcagc
       61 aggcgcggct ccgggcggcg gcgtcgacac gcgggacgcc gagatgccgg ccaccgagaa
      121 ggacctggcg gaggacgcgc catggaagaa gatccagcag aacactttca cgcgctggtg
      181 caacgagcac ctgaagtgcg tgagcaagcg catcgccaac ctgcagacgg acctgagcga
      241 cgggctgcgg cttatcgcgc tgttggaggt gctcagccag aagaagatgc accgcaagca
      301 caaccagcgg cccactttcc gccaaatgca gcttgagaac gtgtcggtgg cactcgagtt
      361 cctggaccgc gagagcatca aactggtgtc catcgacagc aaggccatcg tggacgggaa
      421 cctgaagctg atcctgggcc tcatctggac cctgatcctg cactactcca tctccatgcc
      481 catgtgggac gaggaggagg atgaggaggc caagaagcag acccccaagc agaggctcct
      541 gggctggatc cagaacaagc tgccgcagct gcccatcacc aacttcagcc gggactggca
      601 gagcggccgg gccctgggcg ccctggtgga cagctgtgcc ccgggcctgt gtcctgactg
      661 ggactcttgg gacgccagca agcccgttac caatgcgcga gaggccatgc agcaggcgga
      721 tgactggctg ggcatccccc aggtgatcac ccccgaggag attgtggacc ccaacgtgga
      781 cgagcactct gtcatgacct acctgtccca gttccccaag gccaagctga agccaggggc
      841 tcccttgcgg cccaaactga acccgaagaa agcccgtgcc tacgggccag gcatcgagcc
      901 cacaggcaac atggtgaaga agcgggcaga gttcactgtg gagaccagaa gtgctggcca
      961 gggagaggtg ctggtgtacg tggaggaccc ggccggacac caggaggagg caaaagtgac
     1021 cgccaataac gacaagaacc gcaccttctc cgtctggtac gtccccgagg tgacggggac
     1081 tcataaggtt actgtgctct ttgctggcca gcacatcgcc aagagcccct tcgaggtgta
     1141 cgtggataag tcacagggtg acgccagcaa agtgacagcc caaggtcccg gcctggagcc
     1201 cagtggcaac atcgccaaca agaccaccta ctttgagatc tttacggcag gagctggcac
     1261 gggcgaggtc gaggttgtga tccaggaccc catgggacag aagggcacgg tagagcctca
     1321 gctggaggcc cggggcgaca gcacataccg ctgcagctac cagcccacca tggagggcgt
     1381 ccacaccgtg cacgtcacgt ttgccggcgt gcccatccct cgcagcccct acactgtcac
     1441 tgttggccaa gcctgtaacc cgagtgcctg ccgggcggtt ggccggggcc tccagcccaa
     1501 gggtgtgcgg gtgaaggaga cagctgactt caaggtgtac acaaagggcg ctggcagtgg
     1561 ggagctgaag gtcaccgtga agggccccaa gggagaggag cgcgtgaagc agaaggacct
     1621 gggggatggc gtgtatggct tcgagtatta ccccatggtc cctggaacct atatcgtcac
     1681 catcacgtgg ggtggtcaga acatcgggcg cagtcccttc gaagtgaagg tgggcaccga
     1741 gtgtggcaat cagaaggtac gggcctgggg ccctgggctg gagggcggcg tcgttggcaa
     1801 gtcagcagac tttgtggtgg aggctatcgg ggacgacgtg ggcacgctgg gcttctcggt
     1861 ggaagggcca tcgcaggcta agatcgaatg tgacgacaag ggcgacggct cctgtgatgt
     1921 gcgctactgg ccgcaggagg ctggcgagta tgccgttcac gtgctgtgca acagcgaaga
     1981 catccgcctc agccccttca tggctgacat ccgtgacgcg ccccaggact tccacccaga
     2041 cagggtgaag gcacgtgggc ctggattgga gaagacaggt gtggccgtca acaagccagc
     2101 agagttcaca gtggatgcca agcacggtgg caaggcccca cttcgggtcc aagtccagga
     2161 caatgaaggc tgccctgtgg aggcgttggt caaggacaac ggcaatggca cttacagctg
     2221 ctcctacgtg cccaggaagc cggtgaagca cacagccatg gtgtcctggg gaggcgtcag
     2281 catccccaac agccccttca gggtgaatgt gggagctggc agccacccca acaaggtcaa
     2341 agtatacggc cccggagtag ccaagacagg gctcaaggcc cacgagccca cctacttcac
     2401 tgtggactgc gccgaggctg gccaggggga cgtcagcatc ggcatcaagt gtgcccctgg
     2461 agtggtaggc cccgccgaag ctgacatcga cttcgacatc atccgcaatg acaatgacac
     2521 cttcacggtc aagtacacgc cccggggggc tggcagctac accattatgg tcctctttgc
     2581 tgaccaggcc acgcccacca gccccatccg agtcaaggtg gagccctctc atgacgccag
     2641 taaggtgaag gccgagggcc ctggcctcag tcgcactggt gtcgagcttg gcaagcccac
     2701 ccacttcaca gtaaatgcca aagctgctgg caaaggcaag ctggacgtcc agttctcagg
     2761 actcaccaag ggggatgcag tgcgagatgt ggacatcatc gaccaccatg acaacaccta
     2821 cacagtcaag tacacgcctg tccagcaggg tccagtaggc gtcaatgtca cttatggagg
     2881 ggatcccatc cctaagagcc ctttctcagt ggcagtatct ccaagcctgg acctcagcaa
     2941 gatcaaggtg tctggcctgg gagagaaggt ggacgttggc aaagaccagg agttcacagt
     3001 caaatcaaag ggtgctggtg gtcaaggcaa agtggcatcc aagattgtgg gcccctcggg
     3061 tgcagcggtg ccctgcaagg tggagccagg cctgggggct gacaacagtg tggtgcgctt
     3121 cctgccccgt gaggaagggc cctatgaggt ggaggtgacc tatgacggcg tgcccgtgcc
     3181 tggcagcccc tttcctctgg aagctgtggc ccccaccaag cctagcaagg tgaaggcgtt
     3241 tgggccgggg ctgcagggag gcagtgcggg ctcccccgcc cgcttcacca tcgacaccaa
     3301 gggcgccggc acaggtggcc tgggcctgac ggtggagggc ccctgtgagg cgcagctcga
     3361 gtgcttggac aatggggatg gcacatgttc cgtgtcctac gtgcccaccg agcccgggga
     3421 ctacaacatc aacatcctct tcgctgacac ccacatccct ggctccccat tcaaggccca
     3481 cgtggttccc tgctttgacg catccaaagt caagtgctca ggccccgggc tggagcgggc
     3541 caccgctggg gaggtgggcc aattccaagt ggactgctcg agcgcgggca gcgcggagct
     3601 gaccattgag atctgctcgg aggcggggct tccggccgag gtgtacatcc aggaccacgg
     3661 tgatggcacg cacaccatta cctacattcc cctctgcccc ggggcctaca ccgtcaccat
     3721 caagtacggc ggccagcccg tgcccaactt ccccagcaag ctgcaggtgg aacctgcggt
     3781 ggacacttcc ggtgtccagt gctatgggcc tggtattgag ggccagggtg tcttccgtga
     3841 ggccaccact gagttcagtg tggacgcccg ggctctgaca cagaccggag ggccgcacgt
     3901 caaggcccgt gtggccaacc cctcaggcaa cctgacggag acctacgttc aggaccgtgg
     3961 cgatggcatg tacaaagtgg agtacacgcc ttacgaggag ggactgcact ccgtggacgt
     4021 gacctatgac ggcagtcccg tgcccagcag ccccttccag gtgcccgtga ccgagggctg
     4081 cgacccctcc cgggtgcgtg tccacgggcc aggcatccaa agtggcacca ccaacaagcc
     4141 caacaagttc actgtggaga ccaggggagc tggcacgggc ggcctgggcc tggctgtaga
     4201 gggcccctcc gaggccaaga tgtcctgcat ggataacaag gacggcagct gctcggtcga
     4261 gtacatccct tatgaggctg gcacctacag cctcaacgtc acctatggtg gccatcaagt
     4321 gccaggcagt cctttcaagg tccctgtgca tgatgtgaca gatgcgtcca aggtcaagtg
     4381 ctctgggccc ggcctgagcc caggcatggt tcgtgccaac ctccctcagt ccttccaggt
     4441 ggacacaagc aaggctggtg tggccccatt gcaggtcaaa gtgcaagggc ccaaaggcct
     4501 ggtggagcca gtggacgtgg tagacaacgc tgatggcacc cagaccgtca attatgtgcc
     4561 cagccgagaa gggccctaca gcatctcagt actgtatgga gatgaagagg taccccggag
     4621 ccccttcaag gtcaaggtgc tgcctactca tgatgccagc aaggtgaagg ccagtggccc
     4681 cgggctcaac accactggcg tgcctgccag cctgcccgtg gagttcacca tcgatgcaaa
     4741 ggacgccggg gagggcctgc tggctgtcca gatcacggat cccgaaggca agccgaagaa
     4801 gacacacatc caagacaacc atgacggcac gtatacagtg gcctacgtgc cagacgtgac
     4861 aggtcgctac accatcctca tcaagtacgg tggtgacgag atccccttct ccccgtaccg
     4921 cgtgcgtgcc gtgcccaccg gggacgccag caagtgcact gtcacaggtg ctggcatcgg
     4981 ccccaccatt cagattgggg aggagacggt gatcactgtg gacactaagg cggcaggcaa
     5041 aggcaaagtg acgtgcaccg tgtgcacgcc tgatggctca gaggtggatg tggacgtggt
     5101 ggagaatgag gacggcactt tcgacatctt ctacacggcc ccccagccgg gcaaatacgt
     5161 catctgtgtg cgctttggtg gcgagcacgt gcccaacagc cccttccaag tgacggctct
     5221 ggctggggac cagccctcgg tgcagccccc tctacggtct cagcagctgg ccccacagta
     5281 cacctacgcc cagggcggcc agcagacttg ggccccggag aggcccctgg tgggtgtcaa
     5341 tgggctggat gtgaccagcc tgaggccctt tgaccttgtc atccccttca ccatcaagaa
     5401 gggcgagatc acaggggagg ttcggatgcc ctcaggcaag gtggcgcagc ccaccatcac
     5461 tgacaacaaa gacggcaccg tgaccgtgcg gtatgcaccc agcgaggctg gcctgcacga
     5521 gatggacatc cgctatgaca acatgcacat cccaggaagc cccttgcagt tctatgtgga
     5581 ttacgtcaac tgtggccatg tcactgccta tgggcctggc ctcacccatg gagtagtgaa
     5641 caagcctgcc accttcaccg tcaacaccaa ggatgcagga gaggggggcc tgtctctggc
     5701 cattgagggc ccgtccaaag cagaaatcag ctgcactgac aaccaggatg ggacatgcag
     5761 cgtgtcctac ctgcctgtgc tgccggggga ctacagcatt ctagtcaagt acaatgaaca
     5821 gcacgtccca ggcagcccct tcactgcccg ggtcacaggt gacgactcca tgcgtatgtc
     5881 ccacctaaag gtcggctctg ctgccgacat ccccatcaac atctcagaga cggatctcag
     5941 cctgctgacg gccactgtgg tcccgccctc gggccgggag gagccctgtt tgctgaagcg
     6001 gctgcgtaat ggccacgtgg ggatttcatt cgtgcccaag gagacggggg agcacctggt
     6061 gcatgtgaag aaaaatggcc agcacgtggc cagcagcccc atcccggtgg tgatcagcca
     6121 gtcggaaatt ggggatgcca gtcgtgttcg ggtctctggt cagggccttc acgaaggcca
     6181 cacctttgag cctgcagagt ttatcattga tacccgcgat gcaggctatg gtgggctcag
     6241 cctgtccatt gagggcccca gcaaggtgga catcaacaca gaggacctgg aggacgggac
     6301 gtgcagggtc acctactgcc ccacagagcc aggcaactac atcatcaaca tcaagtttgc
     6361 cgaccagcac gtgcctggca gccccttctc tgtgaaggtg acaggcgagg gccgggtgaa
     6421 agagagcatc acccgcaggc gtcgggctcc ttcagtggcc aacgttggta gtcattgtga
     6481 cctcagcctg aaaatccctg aaattagcat ccaggatatg acagcccagg tgaccagccc
     6541 atcgggcaag acccatgagg ccgagatcgt ggaaggggag aaccacacct actgcatccg
     6601 ctttgttccc gctgagatgg gcacacacac agtcagcgtc aagtacaagg gccagcacgt
     6661 gcctgggagc cccttccagt tcaccgtggg gcccctaggg gaagggggag cccacaaggt
     6721 ccgagctggg ggccctggcc tggagagagc tgaagctgga gtgccagccg aattcagtat
     6781 ctggacccgg gaagctggtg ctggaggcct ggccattgct gtcgagggcc ccagcaaggc
     6841 tgagatctct tttgaggacc gcaaggacgg ctcctgtggt gtggcttatg tggtccagga
     6901 gccaggtgac tacgaagtct cagtcaagtt caacgaggaa cacattcccg acagcccctt
     6961 cgtggtgcct gtggcttctc cgtctggcga cgcccgccgc ctcactgttt ctagccttca
     7021 ggagtcaggg ctaaaggtca accagccagc ctcttttgca gtcagcctga acggggccaa
     7081 gggggcgatc gatgccaagg tgcacagccc ctcaggagcc ctggaggagt gctatgtcac
     7141 agaaattgac caagataagt atgctgtgcg cttcatccct cgggagaatg gcgtttacct
     7201 gattgacgtc aagttcaacg gtacccacat ccctggaagc cccttcaaga tccgagttgg
     7261 ggagcctggg catggagggg acccaggctt ggtgtctgct tacggagcag gtctggaagg
     7321 cggtgtcaca gggaacccag ctgagttcgt cgtgaacacg agcaatgcgg gagctggtgc
     7381 cctgtcggtg accattgacg gcccctccaa ggtgaagatg gattgccagg agtgccctga
     7441 gggctaccgc gtcacctata cccccatggc acctggcagc tacctcatct ccatcaagta
     7501 cggcggcccc taccacattg ggggcagccc cttcaaggcc aaagtcacag gcccccgtct
     7561 cgtcagcaac cacagcctcc acgagacatc atcagtgttt gtagactctc tgaccaaggc
     7621 cacctgtgcc ccccagcatg gggccccggg tcctgggcct gctgacgcca gcaaggtggt
     7681 ggccaagggc ctggggctga gcaaggccta cgtaggccag aagagcagct tcacagtaga
     7741 ctgcagcaaa gcaggcaaca acatgctgct ggtgggggtt catggcccaa ggaccccctg
     7801 cgaggagatc ctggtgaagc acgtgggcag ccggctctac agcgtgtcct acctgctcaa
     7861 ggacaagggg gagtacacac tggtggtcaa atgggggcac gagcacatcc caggcagccc
     7921 ctaccgcgtt gtggtgccca gcggcagcgc ctcaccggtc gccaccatga gtgcgattaa
     7981 gccagacatg aagatcaacc tccgtatgga aggcaacgta aacgggcacc actttgtgat
     8041 cgacggagat ggtacaggca agccttttga gggaaaacag agtatggatc ttgaagtcaa
     8101 agagggcgga cctctgcctt ttgcctttga tatcctgacc actgcattcc attacggcaa
     8161 cagggtattc gccgaatatc cagaccacat acaagactat tttaagcagt cgtttcctaa
     8221 ggggtattcg tgggaacgaa gcttgacttt cgaagacggg ggcatttgca ttgccagaaa
     8281 cgacataaca atggaagggg acactttcta taataaagtt cgatttcacg gtgtaaactt
     8341 tcccgccaat ggtccagtta tgcagaagaa gacgctgaaa tgggagccct ccactgagaa
     8401 aatgtatgtg cgtgatggag tgctgactgg tgatattcgc atggctttgt tgcttgaagg
     8461 aaatgcccat taccgatgtg acttcagaac tacttacaaa gctaaggaga agggtgtcaa
     8521 gttaccaggc taccactttg tggaccactg cattgagatt ttaagccatg acaaagatta
     8581 caacaaggtt aagctgtatg agcatgctgt tgctcattct ggattgcctg acaatgccag
     8641 acgatccggt agcggtcacg gtactggttc tactggttct ggttcttctg agagtgcgat
     8701 taagccagac atgaagatca acctccgtat ggaaggcaac gtaaacgggc accactttgt
     8761 gatcgacgga gatggtacag gcaagccttt tgagggaaaa cagagtatgg atcttgaagt
     8821 caaagagggc ggacctctgc cttttgcctt tgatatcctg accactgcat tccattacgg
     8881 caacagggta ttcgccgaat atccagacca catacaagac tattttaagc agtcgtttcc
     8941 taaggggtat tcgtgggaac gaagcttgac tttcgaagac gggggcattt gcattgccag
     9001 aaacgacata acaatggaag gggacacttt ctataataaa gttcgatttc acggtgtaaa
     9061 ctttcccgcc aatggtccag ttatgcagaa gaagacgctg aaatgggagc cctccactga
     9121 gaaaatgtat gtgcgtgatg gagtgctgac tggtgatatt cgcatggctt tgttgcttga
     9181 aggaaatgcc cattaccgat gtgacttcag aactacttac aaagctaagg agaagggtgt
     9241 caagttacca ggctaccact ttgtggacca ctgcattgag attttaagcc atgacaaaga
     9301 ttacaacaag gttaagctgt atgagcatgc tgttgctcat tctggattgc ctgacaatgc
     9361 cagacgataa gcggccgcga ctctagatca taatcagcca taccacatt
//
